| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,609 | 0 | A | G | 43.8% | 95.9 / 197.4 | 185 | intergenic (+68/+352) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base A (49/55); new base G (47/34); total (96/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 200 total aligned reads displayed.
CAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTG > NC_000913/3192534‑3192684 | cAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa < 1:3689654/76‑1 (MQ=255) gagTAAACCCTCTTCCAGGAATAATCCCTGGCCCGAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt < 2:554675/76‑1 (MQ=255) agTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTg < 1:2601004/76‑1 (MQ=255) aagaaggtttcattaacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGc > 1:1124429‑M1/22‑76 (MQ=255) tAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa < 2:240765/76‑1 (MQ=255) tAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa < 1:2175505/76‑1 (MQ=255) tAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa > 1:1940045/1‑76 (MQ=255) aaaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa < 1:4841393/76‑1 (MQ=255) aaaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa < 2:3409073/76‑1 (MQ=255) aaaCCATCTGCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa < 2:3424546/76‑1 (MQ=255) aaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa > 1:1822710/1‑76 (MQ=255) aggtttcattaacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAAt > 1:5193592‑M1/18‑76 (MQ=255) ggtttcattaacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATg < 2:2462703‑M1/60‑1 (MQ=255) ccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGa > 2:1577552/1‑76 (MQ=255) tttcattaacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAatgct < 1:4671270‑M1/62‑3 (MQ=255) tctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc < 2:2714775/76‑1 (MQ=255) ctcccAGGAATAATCCCAGGCCCAAACAGCCGGGGATTTTTTATAACTGCGTTCCGCTAAAAAGTGCAAATGTTCa < 1:4450971/73‑1 (MQ=255) ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa > 1:942101/1‑76 (MQ=255) ttCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg > 2:3455668/1‑76 (MQ=255) ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt > 2:4140851/1‑76 (MQ=255) ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt > 2:1140675/1‑76 (MQ=255) taacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTcaggac > 1:5692510‑M1/9‑74 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt < 2:1994045/76‑1 (MQ=255) aacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCtcaggacg < 2:1013703‑M1/69‑4 (MQ=255) aacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCtcaggacg < 1:733164‑M1/69‑4 (MQ=255) aGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgctg > 1:268439/1‑76 (MQ=255) ggAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa < 2:178570/57‑1 (MQ=255) ggAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa > 1:178570/1‑57 (MQ=255) ggAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa < 1:1268078/62‑1 (MQ=255) ggAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:4006153/1‑76 (MQ=255) gcataatCCGTAACCCAAAAAGCGGGGGATTTTTTATATCCGCGTACCGCTAAAAAGTGCAAATGATCAGgttgtt < 2:823971/74‑1 (MQ=255) gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt < 1:2094773/76‑1 (MQ=255) ataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc > 1:4897260/1‑76 (MQ=255) taatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgca > 1:5835717/1‑76 (MQ=255) aatGCCTGGCGTAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag < 2:1616738/76‑1 (MQ=255) agcccTGGCCCAAAACGCCGGGGATTTTTTATAGCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc < 2:5014452/74‑1 (MQ=255) cTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGttt > 1:5041498/1‑76 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg < 2:942101/76‑1 (MQ=255) cAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTg < 1:2951213/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGa < 1:3064083/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGa < 1:4101497/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa < 1:4329506/76‑1 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGAc < 2:4460866/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc < 2:5086161/76‑2 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 1:3123247/76‑1 (MQ=255) aaGCCGGGGATTGTTTATATCCGCGTTCCGATAAAAGGTGCAAATGCGCAGGACGCTGCCGCGTTTTGCGCGAccc > 1:1570815/1‑74 (MQ=255) aGCCGGGGATTTTTTCTATCTGCGTTCCGCTAAAAAGTGCCAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 2:5721212/76‑1 (MQ=255) gCCGTGGATTTTTTATATCTGCGTTCCGCTAACAGGTGCAACTGCTCAGGCCGTTGCAGCGTTTTTCGTGACCGCt < 2:4565543/76‑1 (MQ=255) ccGTGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 2:2659483/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:1013703/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc > 1:819524/1‑76 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg > 2:2279783/1‑76 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg < 1:5263050/76‑1 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg > 2:2039906/1‑76 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg > 2:5439566/1‑46 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg < 1:5439567/46‑1 (MQ=255) ggggATGTTTTATATCTGCGTTCAGCTAAAAGGTGCAAACGCTCAGGACGGTGCACCGTTTTGCGCGACCGCTCgg > 2:674375/1‑76 (MQ=255) gggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg > 1:5510516/1‑76 (MQ=255) ggATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCgggg > 1:3023611/1‑76 (MQ=255) aTTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa > 1:3217313/1‑76 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAg > 2:4201247/1‑76 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 2:5156644/1‑76 (MQ=255) tttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg < 2:2137643/76‑1 (MQ=255) tttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg < 2:5404650/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGc < 2:3023611/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGc > 2:4706100/1‑76 (MQ=255) ttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaa > 1:4741314/1‑76 (MQ=255) tataTCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaa > 2:1913209/1‑76 (MQ=255) ttatCTGCGTGCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa < 2:290930/75‑1 (MQ=255) atatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa > 1:382203/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaat < 1:1972907/76‑2 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:3234336/1‑76 (MQ=255) atCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaatt < 2:607175/76‑3 (MQ=255) atCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaatt > 2:389483/1‑74 (MQ=255) atCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACt > 1:1620923/1‑76 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg > 2:4456980/1‑73 (MQ=255) cTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc < 2:1537854/76‑1 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 1:5107487/1‑74 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 1:4416128/1‑74 (MQ=255) gCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGTGTGACGGCTCGGGGaa < 1:2230451/63‑1 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcctc < 2:2619494/76‑4 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:4172158/1‑76 (MQ=255) cGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATc > 1:2369382/1‑76 (MQ=255) gTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAACACTGCATcc > 2:651803/1‑75 (MQ=255) ttCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctg > 1:4268078/1‑74 (MQ=255) ttCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCag < 2:3217313/76‑1 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:1877734/76‑1 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:3925238/76‑1 (MQ=255) ccGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag < 2:3387401/76‑1 (MQ=255) ccGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag > 1:5851352/1‑76 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga < 2:5325170/76‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa > 1:2195451/1‑70 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa < 2:2195661/70‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa < 2:3308879/76‑1 (MQ=255) tAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAg < 1:4117376/76‑1 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc > 2:2243516/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGCGAAAGc > 2:1293766/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc < 1:2810434/76‑1 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag > 2:5611579/1‑70 (MQ=255) aGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGGAAAACTGCATCAGAGAAAGCATTg > 1:5710626/1‑76 (MQ=255) | CAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTG > NC_000913/3192534‑3192684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |