| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,619 | 0 | A | C | 42.6% | 109.2 / 178.1 | 197 | intergenic (+78/+342) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base A (59/53); new base C (52/31); total (112/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 200 total aligned reads displayed.
CCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCT > NC_000913/3192544‑3192694 | ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGCTTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt > 2:660057/1‑76 (MQ=255) ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt > 1:3997632/1‑76 (MQ=255) tctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc < 2:1113656/76‑1 (MQ=255) ctcccAGGAATAATCCCAGGCCCAAACAGCCGGGGATTTTTTATAACTGCGTTCCGCTAAAAAGTGCAAATGTTCa < 1:4450971/73‑1 (MQ=255) ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa < 1:2942029/76‑1 (MQ=255) ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt > 2:4140851/1‑76 (MQ=255) ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt > 1:2655440/1‑76 (MQ=255) aacacatATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCtcaggacg < 1:733164‑M1/69‑4 (MQ=255) gcataatCCGTAACCCAAAAAGCGGGGGATTTTTTATATCCGCGTACCGCTAAAAAGTGCAAATGATCAGgttgtt < 2:823971/74‑1 (MQ=255) gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt < 1:2094773/76‑1 (MQ=255) ataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc > 1:4897260/1‑76 (MQ=255) taatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgca > 1:1646894/1‑76 (MQ=255) aatGCCTGGCGTAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag < 2:1616738/76‑1 (MQ=255) agcccTGGCCCAAAACGCCGGGGATTTTTTATAGCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc < 2:5014452/74‑1 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTgcagcgt > 1:5207324/1‑74 (MQ=255) cTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGttt > 1:5041498/1‑76 (MQ=255) cTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAttt > 1:5850372/1‑76 (MQ=255) tGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGtttt > 1:4269302/1‑76 (MQ=255) gCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGc > 1:5703546/1‑76 (MQ=255) cccAAAACGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg < 1:2306890/76‑1 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCg > 2:3446698/1‑76 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGa < 1:3064083/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGa < 1:4101497/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa < 1:4329506/76‑1 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAc > 2:2632219/1‑76 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAc > 2:1972907/1‑76 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc < 2:5086161/76‑2 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 1:3123247/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCGAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 2:232189/76‑1 (MQ=255) aGCCTGGGATTTTTTATAGCTGCGTTCCGCTCAAAAGTGCCACTGATCAGGTTGTTGCAGCATTTTGGGTGACGGc < 2:751656/76‑1 (MQ=255) aGCCGGGGATTTTTTCTATCTGCGTTCCGCTAAAAAGTGCCAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 2:5721212/76‑1 (MQ=255) gCCGTGGATTTTTTATATCTGCGTTCCGCTAACAGGTGCAACTGCTCAGGCCGTTGCAGCGTTTTTCGTGACCGCt < 2:4565543/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc > 1:819524/1‑76 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:1013703/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:2795790/76‑1 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg > 2:2039906/1‑76 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg > 2:2279783/1‑76 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGGTGCAGCGTTTTGCGTGACCGCTCg > 2:2688706/1‑76 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg < 1:5439567/46‑1 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg > 2:5439566/1‑46 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg < 2:5797169/76‑1 (MQ=255) ggATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCgggg > 1:3023611/1‑76 (MQ=255) ggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggg < 2:3538262/76‑1 (MQ=255) gATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGa < 1:4034011/76‑1 (MQ=255) gATTTTTTATATCTGCGCTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGC‑GTTTGCGTGACCGCTCGGGGaa < 1:1880510/76‑1 (MQ=255) aTTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa > 1:3217313/1‑76 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 2:5156644/1‑76 (MQ=255) tttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg < 2:2137643/76‑1 (MQ=255) tttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg > 1:158347/1‑76 (MQ=255) tttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg < 2:5404650/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGc < 2:3023611/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGc > 2:4706100/1‑76 (MQ=255) ttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGc > 1:213810/1‑76 (MQ=255) tttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAggaa > 2:4188365/1‑74 (MQ=255) ttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaa > 1:1275581/1‑76 (MQ=255) ttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaa > 1:4741314/1‑76 (MQ=255) ttatCTGCGTGCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa < 2:290930/75‑1 (MQ=255) atatCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaa < 1:4143611/76‑1 (MQ=255) atatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa > 1:382203/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaat < 1:1972907/76‑2 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAacac > 2:4313144/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:3234336/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:5490258/1‑76 (MQ=255) atCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaatt > 2:389483/1‑74 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg > 2:4456980/1‑73 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg < 1:5784742/76‑4 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTg > 1:443330/1‑76 (MQ=255) tCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTg > 2:2801182/1‑76 (MQ=255) cTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc < 2:1537854/76‑1 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGGAGGCAAAATTGcct > 1:4654623/1‑74 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 1:5107487/1‑74 (MQ=255) gCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGTGTGACGGCTCGGGGaa < 1:2230451/63‑1 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAACAGTGCacc > 2:154874/1‑74 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 2:627710/1‑76 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:4172158/1‑76 (MQ=255) ttCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctg > 2:4461767/1‑74 (MQ=255) ttCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCag < 2:3217313/76‑1 (MQ=255) tCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctgg > 2:1822569/1‑73 (MQ=255) ccGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag < 2:3387401/76‑1 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga > 1:989622/1‑76 (MQ=255) gCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaa < 1:1864376/76‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa > 1:2195451/1‑70 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa < 2:2195661/70‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa < 2:3308879/76‑1 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc > 2:5607375/1‑76 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc > 2:2243516/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGCGAAAGc > 2:1293766/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGCAAGc > 2:1474670/1‑76 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAtt < 2:5207323/74‑1 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag < 1:5611581/71‑2 (MQ=255) ggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTg > 1:593975/2‑76 (MQ=255) gTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTgg > 1:3798159/1‑75 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgcgg < 2:1828225/76‑4 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCgg > 1:921367/1‑76 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCgg > 2:5686514/1‑76 (MQ=255) aaaTGAGCAGGACGTTGCAGCGTTTTGCGTGCCCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgcgg > 1:5310474/1‑73 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 2:2566356/1‑76 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 1:2402860/1‑76 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:5483696/1‑76 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:3052090/1‑76 (MQ=255) | CCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCT > NC_000913/3192544‑3192694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |