| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,625 | 0 | T | A | 44.1% | 77.6 / 203.5 | 180 | intergenic (+84/+336) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base T (56/44); new base A (50/29); total (106/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 193 total aligned reads displayed.
CCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCT > NC_000913/3192550‑3192700 | ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt > 1:2655440/1‑76 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt < 2:1994045/76‑1 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt < 2:5030845/76‑1 (MQ=255) gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt < 1:2094773/76‑1 (MQ=255) ataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc > 1:4897260/1‑76 (MQ=255) taatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgca > 1:1646894/1‑76 (MQ=255) taatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgca > 1:5835717/1‑76 (MQ=255) atATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa > 2:2269277‑M1/3‑76 (MQ=255) atATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa < 1:607175‑M1/74‑1 (MQ=255) aatGCCTGGCGTAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag < 2:1616738/76‑1 (MQ=255) atCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTgcagc < 2:4625058/76‑1 (MQ=255) agcccTGGCCCAAAACGCCGGGGATTTTTTATAGCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc < 2:5014452/74‑1 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTgcagcgt > 1:5207324/1‑74 (MQ=255) cTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGttt > 1:5041498/1‑76 (MQ=255) cccAAAACGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg < 1:2306890/76‑1 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCg > 2:3446698/1‑76 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg < 2:942101/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGa < 1:4101497/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa < 1:4329506/76‑1 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAc > 2:2632219/1‑76 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGAc < 2:4460866/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc < 2:5086161/76‑2 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCGAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 2:232189/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGCTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc > 1:3226906/1‑75 (MQ=255) aGCCGGGGATTTTTTCTATCTGCGTTCCGCTAAAAAGTGCCAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 2:5721212/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:1013703/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc > 1:819524/1‑76 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGGTGCAGCGTTTTGCGTGACCGCTCg > 2:2688706/1‑76 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg < 2:5797169/76‑1 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg > 2:3987077/1‑76 (MQ=255) ggggATGTTTTATATCTGCGTTCAGCTAAAAGGTGCAAACGCTCAGGACGGTGCACCGTTTTGCGCGACCGCTCgg > 2:674375/1‑76 (MQ=255) gtaTTTTGTATATCTGCGGTACGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggn < 1:3136036/74‑2 (MQ=255) ggATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCgggg > 1:3023611/1‑76 (MQ=255) gATTTTTTATATCTGCGCTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGC‑GTTTGCGTGACCGCTCGGGGaa < 1:1880510/76‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAg > 2:4201247/1‑76 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 2:5156644/1‑76 (MQ=255) tttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg < 2:2137643/76‑1 (MQ=255) tttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg > 1:158347/1‑76 (MQ=255) tttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg < 2:5404650/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGc > 1:213810/1‑76 (MQ=255) ttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaa > 1:1275581/1‑76 (MQ=255) ttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaa > 1:4741314/1‑76 (MQ=255) tataTCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaa > 2:1913209/1‑76 (MQ=255) tataTCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:4151355/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaat < 1:1972907/76‑2 (MQ=255) tatCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaat > 2:1800543/1‑75 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:5490258/1‑76 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGGAGGCAAAATTGcct > 1:4654623/1‑74 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 2:2981242/1‑74 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 1:4416128/1‑74 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 1:5107487/1‑74 (MQ=255) gCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGTGTGACGGCTCGGGGaa < 1:2230451/63‑1 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 2:627710/1‑76 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:3071072/1‑76 (MQ=255) ttCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctg > 1:4268078/1‑74 (MQ=255) ttCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCag < 2:3217313/76‑1 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:3925238/76‑1 (MQ=255) ccGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag > 1:5851352/1‑76 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga > 2:5439902/1‑76 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga > 1:989622/1‑76 (MQ=255) gCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaa < 1:1864376/76‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa < 2:2195661/70‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa > 1:2195451/1‑70 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa < 2:3308879/76‑1 (MQ=255) tAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAg < 1:4117376/76‑1 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc > 2:2243516/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGCGAAAGc > 2:1293766/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc < 1:2810434/76‑1 (MQ=255) aaggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAt > 2:4502007/4‑76 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAtt < 2:2872922/74‑1 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAtt < 2:5207323/74‑1 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGACTCTGGGAAAGCAtt > 1:4036742/3‑76 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag > 2:5611579/1‑70 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag < 1:5611581/71‑2 (MQ=255) aGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGGAAAACTGCATCAGAGAAAGCATTg > 1:5710626/1‑76 (MQ=255) gTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTgg > 1:3798159/1‑75 (MQ=255) tGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCg > 2:803533/1‑76 (MQ=255) gCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgc < 2:1479254/76‑2 (MQ=255) cAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTg < 2:3026393/76‑1 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgcgg < 2:1828225/76‑4 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgcgg < 2:4325397/76‑4 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCgg > 1:4436147/1‑76 (MQ=255) aaaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg < 1:5439903/76‑1 (MQ=255) aaaTGAGCAGGACGTTGCAGCGTTTTGCGTGCCCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgcgg > 1:5310474/1‑73 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg > 2:3581000/1‑76 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg < 2:1548552/76‑1 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 2:2566356/1‑76 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 1:2402860/1‑76 (MQ=255) tGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggggc > 2:5539625/1‑75 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:3052090/1‑76 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:4169955/1‑76 (MQ=255) ctCAGGGCGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTcc < 2:334345/75‑2 (MQ=255) tCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTg > 1:277072/1‑76 (MQ=255) tCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTg < 1:3820099/76‑1 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTggg > 2:134959/1‑75 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgc > 1:4021653/1‑76 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGGGGGGTCTGgc > 2:2310317/1‑76 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAATGCATCAGAGAAAGCATTGCGTGGGGGCTGgc > 1:1075896/1‑76 (MQ=255) gttgttGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcgc < 1:2617809/76‑1 (MQ=255) ttgttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCt > 1:3056806/1‑76 (MQ=255) | CCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCT > NC_000913/3192550‑3192700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |