| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,626 | 0 | T | C | 45.0% | 86.4 / 194.5 | 181 | intergenic (+85/+335) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base T (56/43); new base C (50/31); total (106/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 191 total aligned reads displayed.
CAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTC > NC_000913/3192551‑3192701 | cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt < 2:1994045/76‑1 (MQ=255) ggAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:4006153/1‑76 (MQ=255) atATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa > 2:2269277‑M1/3‑76 (MQ=255) aatGCCTGGCGTAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag < 2:1616738/76‑1 (MQ=255) atCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTgcagc < 2:4625058/76‑1 (MQ=255) agcccTGGCCCAAAACGCCGGGGATTTTTTATAGCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc < 2:5014452/74‑1 (MQ=255) cTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAttt > 1:5850372/1‑76 (MQ=255) tGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGTTCAGGTTGTTGCAGCAtttt > 2:5086806/1‑76 (MQ=255) gCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGc > 1:5703546/1‑76 (MQ=255) cccAAAACGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg < 1:2306890/76‑1 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCg > 2:3446698/1‑76 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg < 2:942101/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa < 1:4329506/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa > 1:5404651/1‑76 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGAc < 2:4460866/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc > 1:3802625/1‑75 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCGAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 2:232189/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGCTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc > 1:3226906/1‑75 (MQ=255) aaGCCGGGGATTGTTTATATCCGCGTTCCGATAAAAGGTGCAAATGCGCAGGACGCTGCCGCGTTTTGCGCGAccc > 1:1570815/1‑74 (MQ=255) aGCCGGGGATTTTTTCTATCTGCGTTCCGCTAAAAAGTGCCAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 2:5721212/76‑1 (MQ=255) ccGTGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 2:2659483/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:1013703/76‑1 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg < 1:5263050/76‑1 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg < 2:5797169/76‑1 (MQ=255) ggggATGTTTTATATCTGCGTTCAGCTAAAAGGTGCAAACGCTCAGGACGGTGCACCGTTTTGCGCGACCGCTCgg > 2:674375/1‑76 (MQ=255) gtaTTTTGTATATCTGCGGTACGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggn < 1:3136036/74‑2 (MQ=255) ggATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCgggg > 1:3023611/1‑76 (MQ=255) gATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGa < 1:4034011/76‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAg > 2:4201247/1‑76 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg < 1:4691651/76‑1 (MQ=255) tttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg < 2:2137643/76‑1 (MQ=255) tttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg < 2:5404650/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGc < 2:3023611/76‑1 (MQ=255) ttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGc < 1:2029542/76‑1 (MQ=255) tataTCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaa > 2:1913209/1‑76 (MQ=255) tataTCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:4151355/1‑76 (MQ=255) ttatCTGCGTGCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa < 2:290930/75‑1 (MQ=255) atatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa > 1:382203/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:4841754/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:5490258/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:3234336/1‑76 (MQ=255) atCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaatt > 2:389483/1‑74 (MQ=255) atCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACt > 1:1620923/1‑76 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg < 1:5784742/76‑4 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTg > 1:443330/1‑76 (MQ=255) tCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTg > 2:2801182/1‑76 (MQ=255) tCTGCGGTCCTCTAACAGCTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg < 1:5092275/76‑4 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct < 1:1800543/76‑3 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 1:4416128/1‑74 (MQ=255) gCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGTGTGACGGCTCGGGGaa < 1:2230451/63‑1 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcctc < 2:2619494/76‑4 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:3071072/1‑76 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 2:627710/1‑76 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:2194669/1‑76 (MQ=255) ttCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctg > 1:4268078/1‑74 (MQ=255) ttCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCag < 2:3217313/76‑1 (MQ=255) tCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctgg > 2:1822569/1‑73 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:1877734/76‑1 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga > 2:5439902/1‑76 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAAGCAAAACTGCATCAgaga > 1:813535/1‑76 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa > 1:2195661/1‑70 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa < 2:2195661/70‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa < 2:3308879/76‑1 (MQ=255) tAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAg < 1:4117376/76‑1 (MQ=255) gaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc < 2:5198823/75‑1 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc > 2:2243516/1‑76 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc < 1:5180345/76‑1 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGCGAAAGc > 2:1293766/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc < 1:2810434/76‑1 (MQ=255) aaggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAt < 1:1913209/73‑1 (MQ=255) aaggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAt > 2:4502007/4‑76 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAtt < 2:5207323/74‑1 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag > 2:5611579/1‑70 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag < 1:5611581/71‑2 (MQ=255) ggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTg > 1:593975/2‑76 (MQ=255) aGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTg < 1:5037075/76‑1 (MQ=255) tGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCg > 2:803533/1‑76 (MQ=255) gCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgc < 2:1479254/76‑2 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCgcgg < 2:4325397/76‑4 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCgg > 1:921367/1‑76 (MQ=255) aaaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg > 1:2098631/1‑76 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg > 2:3581000/1‑76 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg < 2:1548552/76‑1 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 2:2566356/1‑76 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 1:2402860/1‑76 (MQ=255) tgctCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGt < 2:5069328/73‑1 (MQ=255) tGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggggc > 2:5539625/1‑75 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:3052090/1‑76 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:4169955/1‑76 (MQ=255) ctCAGGGCGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTcc < 2:334345/75‑2 (MQ=255) ctCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTcc < 2:1692173/75‑2 (MQ=255) aTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCt < 1:4600117/76‑1 (MQ=255) tCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTg > 1:277072/1‑76 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGCAGGCCACACTGCCTCAGAGAAAGCATTTCGGGGGGTCTGgc > 1:4061383/1‑76 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTggg > 2:134959/1‑75 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGGGGGGTCTGgc > 2:2310317/1‑76 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAATGCATCAGAGAAAGCATTGCGTGGGGGCTGgc > 1:1075896/1‑76 (MQ=255) gttgttGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcgc < 1:2617809/76‑1 (MQ=255) ttgttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCt > 1:3056806/1‑76 (MQ=255) tgttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGGCTGGCGCtc > 1:1225225/1‑76 (MQ=255) | CAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTC > NC_000913/3192551‑3192701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |