| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,635 | 0 | A | G | 43.1% | 104.4 / 198.7 | 189 | intergenic (+94/+326) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base A (61/46); new base G (50/31); total (111/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 203 total aligned reads displayed.
CTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAAT > NC_000913/3192563‑3192710 | cTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAttt > 1:5850372/1‑76 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCg > 2:3446698/1‑76 (MQ=255) cAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTg < 1:2951213/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGa < 1:3064083/76‑1 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa > 1:5404651/1‑76 (MQ=255) aaaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa < 1:4329506/76‑1 (MQ=255) aaaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAc > 2:2632219/1‑76 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc < 2:5086161/76‑2 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 1:3123247/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCGAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 2:232189/76‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGCTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc > 1:3226906/1‑75 (MQ=255) aaGCCGGGGATTGTTTATATCCGCGTTCCGATAAAAGGTGCAAATGCGCAGGACGCTGCCGCGTTTTGCGCGAccc > 1:1570815/1‑74 (MQ=255) gCCGTGGATTTTTTATATCTGCGTTCCGCTAACAGGTGCAACTGCTCAGGCCGTTGCAGCGTTTTTCGTGACCGCt < 2:4565543/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:1013703/76‑1 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc > 1:819524/1‑76 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTc < 1:2795790/76‑1 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg < 1:5263050/76‑1 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCg > 2:2039906/1‑76 (MQ=255) cGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGGTGCAGCGTTTTGCGTGACCGCTCg > 2:2688706/1‑76 (MQ=255) ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg > 2:3987077/1‑76 (MQ=255) ggggATGTTTTATATCTGCGTTCAGCTAAAAGGTGCAAACGCTCAGGACGGTGCACCGTTTTGCGCGACCGCTCgg > 2:674375/1‑76 (MQ=255) gtaTTTTGTATATCTGCGGTACGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggn < 1:3136036/74‑2 (MQ=255) gATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGa < 1:4034011/76‑1 (MQ=255) gATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGa > 1:3026393/1‑76 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg < 1:4691651/76‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 2:5156644/1‑76 (MQ=255) tttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg < 2:5404650/76‑1 (MQ=255) tttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAggaa > 2:4188365/1‑74 (MQ=255) ttATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaa > 1:1275581/1‑76 (MQ=255) tataTCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaa > 2:1913209/1‑76 (MQ=255) tataTCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:4151355/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAacac > 2:4313144/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:5490258/1‑76 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc > 1:4841754/1‑76 (MQ=255) atCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaatt < 2:607175/76‑3 (MQ=255) atCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACt > 1:1620923/1‑76 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg < 1:5784742/76‑4 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg > 2:4456980/1‑73 (MQ=255) tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTg > 1:443330/1‑76 (MQ=255) tCTGCGGTCCTCTAACAGCTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaattg < 1:5092275/76‑4 (MQ=255) cTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc > 2:3330044/1‑76 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct > 2:2981242/1‑74 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGcct < 1:1800543/76‑3 (MQ=255) gCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGTGTGACGGCTCGGGGaa > 2:2230451/1‑63 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:4172158/1‑76 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTc > 1:2194669/1‑76 (MQ=255) cGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATc > 1:2369382/1‑76 (MQ=255) ttCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctg > 2:4461767/1‑74 (MQ=255) tCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTctgg > 2:1822569/1‑73 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:1877734/76‑1 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:3925238/76‑1 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGCGGAAGGCAAAACTGCATCAgaga > 1:2546170/1‑76 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa < 2:2195451/70‑1 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa > 1:2195661/1‑70 (MQ=255) cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa < 2:3280304/76‑1 (MQ=255) tAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAg < 1:4117376/76‑1 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc < 1:5180345/76‑1 (MQ=255) aaaagGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGc > 2:5607375/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGCGAAAGc > 2:1293766/1‑76 (MQ=255) aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGCAAGc > 2:1474670/1‑76 (MQ=255) aaggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAt < 1:1913209/73‑1 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAtt < 2:5207323/74‑1 (MQ=255) aggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAtt < 2:2872922/74‑1 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaag < 1:5611581/71‑2 (MQ=255) ggTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTg > 1:593975/2‑76 (MQ=255) aGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGGAAAACTGCATCAGAGAAAGCATTg > 1:5710626/1‑76 (MQ=255) aGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTg < 1:5037075/76‑1 (MQ=255) gTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTgg > 1:3798159/1‑75 (MQ=255) tGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCg > 2:803533/1‑76 (MQ=255) gCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGt < 2:5778299/76‑1 (MQ=255) cAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTg < 2:3026393/76‑1 (MQ=255) aaaTGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCgg > 2:5686514/1‑76 (MQ=255) aaaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg > 2:3080049/1‑76 (MQ=255) aaaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg > 1:2098631/1‑76 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg < 2:1548552/76‑1 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg > 2:3581000/1‑76 (MQ=255) aaTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg > 1:2233991/1‑76 (MQ=255) aTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg > 2:2566356/1‑76 (MQ=255) gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc > 2:5483696/1‑76 (MQ=255) ctCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTcc < 2:1692173/75‑2 (MQ=255) aTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCt < 1:4600117/76‑1 (MQ=255) tCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTg < 1:3820099/76‑1 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGCAGGCCACACTGCCTCAGAGAAAGCATTTCGGGGGGTCTGgc > 1:4061383/1‑76 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTggg > 2:134959/1‑75 (MQ=255) aGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAATGCATCAGAGAAAGCATTGCGTGGGGGCTGgc > 1:1075896/1‑76 (MQ=255) ggTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGGCTGgcc > 2:224720/1‑75 (MQ=255) cgttgCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCtc < 1:199257/75‑1 (MQ=255) gttgCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCtca > 1:665396/1‑76 (MQ=255) gttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtca > 1:4410163/1‑76 (MQ=255) gttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtca > 1:967279/1‑76 (MQ=255) ttgCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCtcat > 1:3402547/1‑76 (MQ=255) ttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtcat < 2:1508742/76‑1 (MQ=255) ttgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtcat > 1:1584908/1‑76 (MQ=255) tgCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtcatc < 2:3356027/76‑1 (MQ=255) tgCAGCATTTTGCGTGACGGCTCGAGCGAGAAAAAACGGCAGCAGAGAAAGAATTGCGTGCGCAATGGCGCtcagc > 2:2304648/1‑74 (MQ=255) cagcgTTTTGCGTGACCGCTCGGTGAAGGCAAAATTGCCTCTGGTAAAGCATTGCGCGGGGTCCGGCGCTCATcaa < 1:2697638/76‑1 (MQ=255) agcaTTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATcaac < 2:4841753/76‑1 (MQ=255) agcaTTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATcaac > 1:1204920/1‑76 (MQ=255) caTTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATcaacaa > 2:2600575/1‑76 (MQ=255) caTTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCCCATcaacac > 2:628586/1‑75 (MQ=255) | CTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAAT > NC_000913/3192563‑3192710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |