| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,757 | 0 | G | . | 43.5% | 268.3 / 290.1 | 171 | intergenic (+216/+204) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base G (39/57); new base . (37/37); total (77/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 203 total aligned reads displayed.
ATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCA > NC_000913/3192681‑3192832 | ttGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAgg < 1:3185804/76‑1 (MQ=25) ttGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAgg < 1:2065941/76‑1 (MQ=25) tggCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGaagaag < 2:1599367/74‑1 (MQ=255) tGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAggg < 1:879340/76‑1 (MQ=21) tGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAggg < 1:2599652/76‑1 (MQ=25) cGTGGGGTCTGGCGCTCATCAACAGTCGGGGAGTCGTCAGGGGGCTGAAACGGTAAAGCCCCTCCCGAAGAAgggg < 2:4962114/76‑1 (MQ=37) aggggggTCTGGCGCTCATAAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGCAAAGGCCCTCCCCCAGAAgggg > 2:4378915/4‑76 (MQ=255) tGGGGTCTGGACCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGGAGAAGGGGGc > 2:4579652/1‑76 (MQ=37) gggTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCt < 2:1103030/76‑1 (MQ=255) gTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTa < 2:3997631/76‑1 (MQ=255) tGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAg < 2:4410162/76‑1 (MQ=255) tGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAg > 1:988667/1‑76 (MQ=255) gcgcTCATCAACAATCGGGGAGTCAGCAGGAGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGaa < 2:876347/76‑1 (MQ=255) gcTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAgg < 1:1474670/76‑1 (MQ=255) gcTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGaa > 2:4624200/1‑76 (MQ=17) cTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGaaa > 1:225936/1‑76 (MQ=12) tcatcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa > 2:599994/1‑76 (MQ=255) tcatcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa < 1:3927559/76‑1 (MQ=255) tcatcaACAATCGGGGAGGCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa < 2:5403955/76‑1 (MQ=255) tcatcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAG‑ACGGGGCCTTGTATAAGGAAAg > 2:308807/1‑76 (MQ=12) catcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaaa < 1:1173613/76‑1 (MQ=255) catcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCCAAGAA‑GGGGCCTTGTCTAAGGAAAgg > 2:169012/1‑76 (MQ=12) atcaCCAATCGGGGTGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAg < 2:1822710/76‑1 (MQ=255) atcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAg < 1:5156645/76‑1 (MQ=255) tcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAgg < 2:5149432/76‑1 (MQ=255) tcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGt > 1:3806909/1‑76 (MQ=9) tcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGt > 1:32896/1‑76 (MQ=9) caacaaTCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTCCAGCAGGAAAggg > 2:3026947/1‑76 (MQ=255) aacaaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTa > 1:5773967/1‑76 (MQ=9) aacaaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGGTa > 1:1041603/1‑76 (MQ=9) acaaTCGGGGAGTCGGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGta < 2:3452232/76‑2 (MQ=255) acaaTCGGGGAGTCAGCGGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGtt < 2:2380107/76‑1 (MQ=255) aaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatga > 2:4814260/1‑76 (MQ=9) aTCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatg > 1:3448978/1‑76 (MQ=255) aTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatgat < 1:2575299/76‑1 (MQ=9) tCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatga < 2:2919861/76‑1 (MQ=255) cGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatgat > 1:4341183/1‑76 (MQ=255) ggggAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatgatg > 1:4063453/1‑76 (MQ=255) ggggAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatgatg > 2:1768825/1‑76 (MQ=255) ggggAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCACCCGAAGAAGGGGGCCTTACAGTAGGAAAGGGGTatgatg > 1:5287527/1‑76 (MQ=38) ggcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 2:2030187/74‑1 (MQ=14) ggcagcaaGGGGCTGAAACGGGAAAGCCCCGCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 1:305657/74‑1 (MQ=14) gTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCt < 1:5833305/76‑1 (MQ=255) gTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGGTATGATGAAGCt > 2:1431755/1‑76 (MQ=255) gcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg < 2:1407759/75‑1 (MQ=14) agcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGt > 1:4306183/1‑76 (MQ=255) agcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGt > 1:2756823/1‑76 (MQ=255) agcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 2:665396/76‑1 (MQ=17) caaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 2:1275581/73‑1 (MQ=17) caaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:4866630/4‑76 (MQ=17) agGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcat > 2:4205294/1‑76 (MQ=255) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc > 2:4607176/3‑76 (MQ=17) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc > 1:5635318/3‑76 (MQ=17) aggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatca > 2:4882152/2‑76 (MQ=17) ggggTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa < 2:5685444/76‑1 (MQ=14) gggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTcatcat > 1:5022104/1‑76 (MQ=255) gggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:1317570/1‑76 (MQ=17) ggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 2:1230714/1‑76 (MQ=17) ggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 1:537859/1‑76 (MQ=17) ggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 1:4063985/76‑1 (MQ=17) ggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTAATGATGAAGCTCGCcatcatcc > 2:4165975/1‑74 (MQ=11) gCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt > 1:3307172/1‑76 (MQ=14) gCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTACGATGAAGCTCCTCATCATACt > 2:436426/1‑76 (MQ=14) cTGAAACGGGATAGCCCTGCCCGAAGAA‑GGTGCCTTGTATAAGGAAAGGGTTATGATGACGCTCGTCATCATACTg < 1:4290285/76‑1 (MQ=9) cTGAAACCGGAAAGCCCCTCACGAAGAAGGGCGCCTTACAGAAGGAAAGGGTCATGATGAAGCTCGTCATCATACt > 2:3927083/1‑76 (MQ=21) caCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGACCTCATACTGGTtg > 1:2844886/2‑76 (MQ=14) aaggggAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGAtt < 2:5710624/73‑1 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtg < 1:4814261/76‑1 (MQ=14) aCGTGAAGGCACCTCCCGAAGAA‑GGGTCCTTTTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 1:177932/76‑1 (MQ=9) aCGGGAACGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:3226906/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTTATCATACTGGTtgt < 1:154874/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:2327305/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 1:4201248/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt > 2:2944884/1‑76 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:733164/76‑1 (MQ=14) gggAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATtgtg < 1:2310317/76‑1 (MQ=255) gtaaaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTgtgt < 1:2801182/74‑1 (MQ=255) gAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgtt < 1:3737645/76‑1 (MQ=255) aaaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGt < 1:4527567/42‑1 (MQ=255) aaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttgt < 2:5510514/76‑1 (MQ=255) ccccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGCTATGATGAAGCTCGTCATCATACTGATTGTGTTCTTAc > 1:2719737/1‑76 (MQ=255) cccTCCCGAAGAAGGGGGCCTTACGGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACt > 1:695223/1‑76 (MQ=255) cccTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACtg > 1:2425947/1‑75 (MQ=18) cccTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACtg < 1:1828438/76‑2 (MQ=18) cccGCCGGAAGAAGGGGG‑CTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACtg < 2:4143/76‑2 (MQ=14) ccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTc < 2:2655440/76‑1 (MQ=255) cTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCg < 2:213810/76‑1 (MQ=255) tCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttg < 2:558758/68‑1 (MQ=255) tCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttg > 1:558758/1‑68 (MQ=255) cccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGGCATTATACTGATTGTGTTGTTACTCGtc > 1:2659483/1‑75 (MQ=38) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt > 2:4156343/1‑76 (MQ=255) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt < 1:599994/76‑1 (MQ=255) gaagaa‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt < 1:2632219/76‑1 (MQ=12) aagaa‑gGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt > 2:1516079/1‑76 (MQ=17) gaagGGTGCCTTACAGCAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTGCTCGTAGGTTTc < 2:2180259/76‑1 (MQ=25) gaagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc < 1:361309/76‑1 (MQ=255) gaagGGGGCCGTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc < 2:5835716/76‑1 (MQ=255) agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGc > 2:1889576/1‑76 (MQ=255) gggggCCTTGCAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTCCTCGTAAGTTTCGCa < 2:2546170/76‑1 (MQ=255) gggggACTTAAAGAAGGAAAGGGTGATGATGAGGGTCGTGAGCATGCTGATTGTTTTGTTCCTCGTAAGTTTCGCa < 2:4741313/76‑1 (MQ=255) | ATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCA > NC_000913/3192681‑3192832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |