| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,766 | 0 | A | G | 41.6% | 212.6 / 188.1 | 192 | intergenic (+225/+195) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base A (45/66); new base G (40/39); total (86/106) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 197 total aligned reads displayed.
GTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAA > NC_000913/3192691‑3192841 | ggCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGa < 2:1698083/76‑1 (MQ=255) ggCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGa < 1:5425060/76‑1 (MQ=255) gcTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAgg < 2:849748/76‑1 (MQ=255) gcTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAgg < 1:1474670/76‑1 (MQ=255) gcTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGaa > 2:4624200/1‑76 (MQ=17) cTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTCACAGAAGGa > 2:1778994/1‑76 (MQ=255) tcatcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa > 2:599994/1‑76 (MQ=255) tcatcaACAATCGGGGAGGCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa < 2:5403955/76‑1 (MQ=255) tcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGt < 2:4386162/76‑1 (MQ=9) tcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGt > 1:32896/1‑76 (MQ=9) caacaaTCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTCCAGCAGGAAAggg > 2:3026947/1‑76 (MQ=255) aacaaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTa > 1:5773967/1‑76 (MQ=9) aacaaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGGTa > 1:1041603/1‑76 (MQ=9) acaaTCGGGGAGTCAGCGGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGtt < 2:2380107/76‑1 (MQ=255) aaTCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTat < 1:4140852/76‑1 (MQ=255) aaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatga > 2:4814260/1‑76 (MQ=9) aaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatga < 1:5607377/76‑1 (MQ=9) aTCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatg > 1:3448978/1‑76 (MQ=255) aTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatgat < 1:2575299/76‑1 (MQ=9) tCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatga < 2:2919861/76‑1 (MQ=255) ggcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 2:2030187/74‑1 (MQ=14) ggcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 2:880759/74‑1 (MQ=14) gTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCt > 1:1794060/1‑76 (MQ=255) gTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGGTATGATGAAGCt > 2:1431755/1‑76 (MQ=255) tCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTc < 1:4976629/76‑1 (MQ=255) agcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGt > 1:3721869/1‑76 (MQ=255) agcagGGGGCGGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGACTTACAGAAGGAAAGGGTTATGATGAAGCGCGt > 2:1906481/1‑76 (MQ=25) agcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 1:2799079/76‑1 (MQ=17) agcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 2:665396/76‑1 (MQ=17) gcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtc < 2:3179802/76‑1 (MQ=255) caaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 2:1275581/73‑1 (MQ=17) caaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 1:2279783/73‑1 (MQ=17) agagggCTGAAACGGGAGAGCGCCTCCCGAAGCAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcat < 2:4150531/73‑1 (MQ=25) agGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcat > 2:4205294/1‑76 (MQ=255) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc > 1:2327305/3‑76 (MQ=17) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc > 2:4607176/3‑76 (MQ=17) aggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatca > 2:4882152/2‑76 (MQ=17) ggggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcatca < 1:5086807/76‑1 (MQ=255) ggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat > 2:1828438/1‑76 (MQ=17) ggggTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa < 2:5685444/76‑1 (MQ=14) gggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:4063541/1‑76 (MQ=17) gggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:1317570/1‑76 (MQ=17) ggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTAATGATGAAGCTCGCcatcatcc > 2:4165975/1‑74 (MQ=11) gCTGAAACTGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 2:4006152/76‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 2:359170/76‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 1:1140675/76‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt > 1:3307172/1‑76 (MQ=14) gCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt < 1:969524/76‑1 (MQ=14) gCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTACGATGAAGCTCCTCATCATACt > 2:436426/1‑76 (MQ=14) cTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt > 2:3928310/1‑76 (MQ=255) cTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt > 1:3182335/1‑76 (MQ=255) cTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 2:5041497/76‑1 (MQ=17) aaggggAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGAtt < 2:5710624/73‑1 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtg > 1:2824950/1‑76 (MQ=14) aaCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtg < 1:4814261/76‑1 (MQ=14) aCGGGAACGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:3226906/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:733164/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt > 2:3042256/1‑76 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt > 2:2944884/1‑76 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:2327305/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 1:2688706/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGGTATGATGAAGCTCGTCATCATACTGGTtgt > 1:1735611/1‑76 (MQ=14) ggAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGtgtt > 1:4360723/1‑76 (MQ=17) ggAAAGCCCCGCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGtgtt > 1:5350255/1‑76 (MQ=17) aaaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGt > 2:4527566/1‑42 (MQ=255) aaaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGt < 1:4527567/42‑1 (MQ=255) aaaGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:3845918/76‑1 (MQ=17) aaGCCGCTCCCGTAGATGGGTGCCTTACAGAAGGAAAGGGTTATGATGACGCTCGTCATCATACTGATTGtgttgt < 1:2728100/76‑1 (MQ=25) aaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttgt < 1:2851126/76‑1 (MQ=255) aaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttgt < 2:5510514/76‑1 (MQ=255) cccTCCCGAAGAAGGGGGCCTTACGGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACt > 1:695223/1‑76 (MQ=255) cccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACt < 1:3743124/76‑1 (MQ=255) cccTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACtg > 1:2655101/1‑75 (MQ=18) ccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTc < 2:2655440/76‑1 (MQ=255) cTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCg < 2:213810/76‑1 (MQ=255) tCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttg < 2:558758/68‑1 (MQ=255) ccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTaa < 2:1075896/76‑1 (MQ=255) ccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTaa < 1:2115325/76‑1 (MQ=255) ccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTaa < 1:4205295/76‑1 (MQ=255) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt < 1:599994/76‑1 (MQ=255) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt > 1:2619494/1‑76 (MQ=255) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt > 2:4156343/1‑76 (MQ=255) aagaa‑gGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt > 2:1516079/1‑76 (MQ=17) gaagGGTGCCTTACAGCAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTGCTCGTAGGTTTc < 2:2180259/76‑1 (MQ=25) gaagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc > 1:4298627/1‑76 (MQ=255) gaagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc < 2:1620923/76‑1 (MQ=255) gaagGGGGCCGTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc < 2:5835716/76‑1 (MQ=255) agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGc < 1:4313145/76‑1 (MQ=255) agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGc > 1:5190207/1‑76 (MQ=255) gggggCCTTGCAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTCCTCGTAAGTTTCGCa < 2:2546170/76‑1 (MQ=255) gggggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCa < 1:3987078/76‑1 (MQ=255) gggggACTTAAAGAAGGAAAGGGTGATGATGAGGGTCGTGAGCATGCTGATTGTTTTGTTCCTCGTAAGTTTCGCa < 2:4741313/76‑1 (MQ=255) gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGc < 1:2566356/76‑1 (MQ=255) gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGc < 1:4358147/76‑1 (MQ=255) ggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCt < 2:1335796/76‑1 (MQ=255) gCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCtt > 2:3861513/1‑76 (MQ=255) cTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttat > 1:2014441/1‑76 (MQ=255) ttACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttatt > 2:3328698/1‑76 (MQ=255) tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttatta > 2:5193591/1‑76 (MQ=255) tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttatta < 1:3581000/76‑1 (MQ=255) | GTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAA > NC_000913/3192691‑3192841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |