| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,769 | 0 | G | T | 41.0% | 148.6 / 190.6 | 190 | intergenic (+228/+192) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base G (46/66); new base T (39/39); total (85/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 193 total aligned reads displayed.
TGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAA > NC_000913/3192694‑3192841 | tGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAg < 2:4410162/76‑1 (MQ=255)tGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAg > 1:988667/1‑76 (MQ=255)gggCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAg < 2:1298540/75‑1 (MQ=255) ggCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGa < 1:5425060/76‑1 (MQ=255) gcTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAgg < 2:849748/76‑1 (MQ=255) gcTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAgg < 1:1474670/76‑1 (MQ=255) tcatcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa > 2:599994/1‑76 (MQ=255) tcatcaACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa < 1:3927559/76‑1 (MQ=255) tcatcaACAATCGGGGAGGCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGaa < 2:5403955/76‑1 (MQ=255) tcatcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAg > 2:2155785/1‑76 (MQ=17) catcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAgg < 1:4457299/76‑1 (MQ=12) catcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCCAAGAA‑GGGGCCTTGTCTAAGGAAAgg > 2:169012/1‑76 (MQ=12) atcaCCAATCGGGGTGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAg < 2:1822710/76‑1 (MQ=255) tcaACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGt > 1:3806909/1‑76 (MQ=9) aacaaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGGTa > 1:1041603/1‑76 (MQ=9) acaaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGCTat > 2:2276584/1‑76 (MQ=9) acaaGCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGtt < 2:155713/76‑1 (MQ=255) caaTGGGGGAGTCGGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTa < 2:4203319/76‑1 (MQ=255) aaTCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTat < 1:4140852/76‑1 (MQ=255) aaTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatga < 1:5607377/76‑1 (MQ=9) aTCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTatgat < 1:2575299/76‑1 (MQ=9) ggggAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatgatg > 2:1768825/1‑76 (MQ=255) ggggAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTatgatg > 1:4063453/1‑76 (MQ=255) aGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGc > 2:2534255/1‑76 (MQ=255) ggcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 2:2030187/74‑1 (MQ=14) ggcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 2:880759/74‑1 (MQ=14) ggcagcaaGGGGCTGAAACGGGAAAGCCCCGCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 1:305657/74‑1 (MQ=14) gTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCt < 1:5833305/76‑1 (MQ=255) gTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCt > 1:1794060/1‑76 (MQ=255) tCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTc < 1:660057/76‑1 (MQ=255) tCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTc < 1:4976629/76‑1 (MQ=255) gcagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg < 2:1407759/75‑1 (MQ=14) gagcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt < 2:4372160/75‑1 (MQ=14) agcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGt > 1:2756823/1‑76 (MQ=255) agcaaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 2:665396/76‑1 (MQ=17) gcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtc < 2:3179802/76‑1 (MQ=255) gcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGATCGtc > 2:147023/1‑76 (MQ=255) gcaaGGGGCTGAACCGGGAATGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGCAGCTCGtca < 1:3482142/76‑1 (MQ=12) caaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 1:2279783/73‑1 (MQ=17) agagggCTGAAACGGGAGAGCGCCTCCCGAAGCAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcat < 2:4150531/73‑1 (MQ=25) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc < 2:4654580/74‑1 (MQ=17) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc > 1:5635318/3‑76 (MQ=17) aaggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc > 1:2327305/3‑76 (MQ=17) gggggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcatc < 2:268439/76‑1 (MQ=255) aggggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatca > 2:4882152/2‑76 (MQ=17) ggggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGtcatca < 1:5086807/76‑1 (MQ=255) ggggTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa < 2:5685444/76‑1 (MQ=14) gggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa < 1:744285/76‑1 (MQ=17) gggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:4063541/1‑76 (MQ=17) gggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:1317570/1‑76 (MQ=17) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATa < 2:2369382/76‑1 (MQ=255) ggCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 2:1230714/1‑76 (MQ=17) gCTGAAACTGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 2:4006152/76‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 2:359170/76‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 1:1140675/76‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt > 1:3266276/1‑76 (MQ=14) cTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 2:5041497/76‑1 (MQ=17) cTGAAACCGGAAAGCCCCTCACGAAGAAGGGCGCCTTACAGAAGGAAAGGGTCATGATGAAGCTCGTCATCATACt > 2:3927083/1‑76 (MQ=21) tGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg > 2:2931899/1‑76 (MQ=255) tGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg > 2:2958681/1‑76 (MQ=255) tGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGGTATGATGACGCTCGTCATCATACTgg > 2:2583254/1‑75 (MQ=14) aaaCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTggtt < 1:3446698/76‑4 (MQ=14) caCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGACCTCATACTGGTtg > 1:2844886/2‑76 (MQ=14) aaggggAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGAtt < 2:5710624/73‑1 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtg < 1:4814261/76‑1 (MQ=14) aCGGGAACGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:3226906/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 2:733164/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt > 2:4612035/1‑76 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt > 2:3042256/1‑76 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTtgt < 1:2688706/76‑1 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGGTATGATGAAGCTCGTCATCATACTGGTtgt > 1:1735611/1‑76 (MQ=14) gtaaaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTgtgt < 1:2801182/74‑1 (MQ=255) ggAAAGCCCCGCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGtgtt > 1:5350255/1‑76 (MQ=17) gAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgtt < 1:3737645/76‑1 (MQ=255) gAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgtt > 2:486716/1‑76 (MQ=255) aaGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttgt < 2:5510514/76‑1 (MQ=255) ccccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGCTATGATGAAGCTCGTCATCATACTGATTGTGTTCTTAc > 1:2719737/1‑76 (MQ=255) cccTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACtg > 1:2655101/1‑75 (MQ=18) ccTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACtgt > 1:3307005/1‑74 (MQ=18) tCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGtgttg < 2:558758/68‑1 (MQ=255) cccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTa > 2:4765522/1‑76 (MQ=255) ccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTaa < 1:4205295/76‑1 (MQ=255) ccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTaa < 1:2115325/76‑1 (MQ=255) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt > 1:2619494/1‑76 (MQ=255) gaagaaGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGt > 2:4156343/1‑76 (MQ=255) aagaagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGtt > 2:4596144/1‑76 (MQ=255) gaagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc < 1:361309/76‑1 (MQ=255) gaagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc > 1:4298627/1‑76 (MQ=255) gaagGGGGCCGTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTc < 2:5835716/76‑1 (MQ=255) aagGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCg < 2:4217637/76‑1 (MQ=255) agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGc > 1:5190207/1‑76 (MQ=255) agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGc > 2:1889576/1‑76 (MQ=255) agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGc < 1:4313145/76‑1 (MQ=255) gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGc > 2:3600138/1‑76 (MQ=255) gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGc < 1:2566356/76‑1 (MQ=255) gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGc < 1:4358147/76‑1 (MQ=255) gCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCtt > 2:1992673/1‑76 (MQ=255) cTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttat > 1:2014441/1‑76 (MQ=255) ttACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttatt > 2:3328698/1‑76 (MQ=255) tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCttatta > 2:5193591/1‑76 (MQ=255) | TGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAA > NC_000913/3192694‑3192841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |