| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,873 | 0 | T | C | 53.4% | 16.0 / 183.7 | 148 | intergenic (+332/+88) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base T (34/35); new base C (38/41); total (72/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 155 total aligned reads displayed.
TCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGA > NC_000913/3192798‑3192948 | tcatACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAATTCCTCCCtt < 2:3402547/76‑1 (MQ=255)tcatACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCtt < 1:2976912/76‑1 (MQ=255)tcatACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCtt > 2:5529997/1‑76 (MQ=255) catACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGGAATCCTCCCTTa > 2:5783772/1‑76 (MQ=255) attcTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTAc < 2:2060521/73‑1 (MQ=255) tACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTAcc < 1:3664208/76‑1 (MQ=255) aCTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTAccc < 1:3447954/76‑1 (MQ=255) aCTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGTAATCCTCCCTCAccc > 1:2401634/1‑76 (MQ=255) aCTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCAccc < 2:4155935/76‑1 (MQ=255) cTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCt > 1:3246920/1‑76 (MQ=255) tggttGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCtt < 2:2779976/73‑1 (MQ=255) tggttGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCtt > 2:4118310/4‑76 (MQ=255) tGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCtt < 2:277072/76‑1 (MQ=255) ggttGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCACAGGGGGGAGAAATCCTCCCTTACCCttc > 2:262529/3‑75 (MQ=255) gttGTGTTGTTACTGTTCAGTTTCCCGACTTACTAACAACTCATCCGAGGGGGGAGAAATCCTCCCTTACCATTGt > 1:3424750/2‑76 (MQ=255) gttGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGt > 2:5207623/2‑76 (MQ=255) aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGt < 1:3674095/76‑1 (MQ=255) ttGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTCCCCTTGtt < 2:3961858/76‑1 (MQ=255) ttGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGtt < 1:3790652/76‑1 (MQ=255) ttGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGtt > 1:4794042/1‑76 (MQ=255) tggtgtTGTTACTGTTAAGTTTCCCGCCTTCCTCACAACTCATCAGAGGGGGGAGAAATCCTCCCTTCCCCTTGtt < 2:554027/74‑1 (MQ=255) tgtgTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTc > 1:863545/1‑76 (MQ=255) tgtgTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTc > 2:4788842/1‑76 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTc < 1:3434028/76‑1 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTc < 1:651803/76‑1 (MQ=255) tgttgtTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCt > 1:3284332/1‑76 (MQ=255) tgttgtTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTcat < 1:134959/76‑3 (MQ=255) ttgttACTGTTAAGTTTCCCGACTTACTAACACCTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCttt < 1:1030272/76‑1 (MQ=255) ttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCttt > 1:5086162/1‑76 (MQ=255) tttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTAc < 1:1431755/75‑1 (MQ=255) gttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTAc < 2:1505395/76‑1 (MQ=255) aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTActtt < 1:4837188/76‑3 (MQ=255) aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTActtt > 2:4932248/1‑74 (MQ=255) aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTActtt > 1:5069329/1‑74 (MQ=255) cTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttacttta > 2:4900241/1‑76 (MQ=255) cGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAgg < 2:2143906/76‑1 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTg > 2:3674253/1‑76 (MQ=255) gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaa < 2:3335070/76‑1 (MQ=255) gtgcGCAGCTTATTAACAGCCAATCGGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaa < 2:2674650/73‑1 (MQ=255) ttCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaa < 1:980202/76‑1 (MQ=255) gCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaa > 1:4192407/1‑75 (MQ=255) gCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaa > 1:5338076/1‑75 (MQ=255) gCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaa > 1:2872922/1‑75 (MQ=255) cAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaat > 2:5092274/1‑74 (MQ=255) cAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaaa < 2:1516926/76‑3 (MQ=255) acTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaac < 2:1474096/75‑1 (MQ=255) attaACAGCCAAGCAGAGGGGGTAGAAATCCTCACTCACCCTTGTGCCTTTACTGTAGGTTGAAAAAAAATCAGCg < 1:4523450/76‑1 (MQ=255) taACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTc > 1:5193488/1‑76 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCa < 2:1206338/76‑1 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTCTTCCTTTACTTTAGGGTGAAAAAAAATCAGCGTCa > 1:379061/1‑76 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa > 2:596499/1‑76 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAg > 1:3134629‑M2/1‑75 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAg > 2:4341370‑M2/1‑75 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGa > 2:2407461/1‑57 (MQ=255) ctcaTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAgg < 1:2832543/73‑1 (MQ=255) ccAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaag < 2:3030849‑M2/76‑5 (MQ=255) ccAATCAGAGGGGGGAGAAATCCTCCCTCACCCTAGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaag < 2:5784741‑M2/76‑5 (MQ=255) cAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaagg > 1:1778994‑M2/1‑71 (MQ=255) caTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGcc > 1:4916035/2‑76 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt > 2:2347034/1‑76 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt < 1:3104190/76‑1 (MQ=255) tCAGAGGGGGGAGAAATCCTCCGTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:5287526‑M2/1‑68 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:3170311‑M2/1‑68 (MQ=255) tCAGAGGGGGGAGAAAGCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAACAACAACAGCGTCAATAGGCCTg < 2:3266276/76‑1 (MQ=255) agagGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGcc > 1:3501149/1‑76 (MQ=255) gggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTGGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCAt < 2:2259961/75‑1 (MQ=255) ggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATg > 1:2502287/1‑76 (MQ=255) ggggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcatta > 1:5604813‑M2/1‑63 (MQ=255) gggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGt < 1:4041093/76‑1 (MQ=255) gggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGt < 2:3806908/76‑1 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaa > 1:2534255‑M2/1‑62 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGCTGAAAAAAAATCAGCGTCAAgaaggtttcattaa < 2:3034630‑M2/76‑15 (MQ=255) gggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTAc > 1:4531273/1‑76 (MQ=255) gggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTAc < 1:4705556/76‑1 (MQ=255) gggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaaca < 1:1813301‑M2/76‑17 (MQ=255) gggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaaca > 2:4801021‑M2/1‑60 (MQ=255) ggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACg > 1:67830/1‑76 (MQ=255) ggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACg > 2:3196644/1‑76 (MQ=255) ggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacac < 2:1880510‑M2/76‑18 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa < 2:4916034/76‑1 (MQ=255) agaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGaa < 2:4287075/76‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacat < 2:3743578‑M2/76‑20 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacat < 2:1647068‑M2/76‑20 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTCCTCTCGGTTGAACAACCAACAGCGTCCATAGGCCTGCCCTGTACGCAg > 2:4640467/1‑76 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg > 2:4586379/1‑76 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg < 1:293087/76‑1 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacata > 1:4156344‑M2/1‑56 (MQ=255) aaaTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc < 2:32896/76‑1 (MQ=255) aaaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatat < 2:1111562‑M2/76‑22 (MQ=255) aaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatc < 2:5833918‑M2/76‑23 (MQ=255) aTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAACAGGCCTGACATGTACGAAGCga > 2:3958063/1‑76 (MQ=255) aTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatcc > 2:5338075‑M2/1‑53 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag < 2:863545/76‑1 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag < 1:5783773/76‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:1428058‑M2/1‑52 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:5395404‑M2/1‑52 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccct > 1:2958681‑M2/1‑51 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccct > 1:3674253‑M2/1‑51 (MQ=255) ccTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcc < 2:4463310‑M2/76‑30 (MQ=255) tACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGa > 1:215040/1‑76 (MQ=255) | TCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGA > NC_000913/3192798‑3192948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |