| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,891 | 0 | C | T | 51.9% | 45.9 / 183.4 | 159 | intergenic (+350/+70) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base C (33/43); new base T (43/39); total (76/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 171 total aligned reads displayed.
CTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCC > NC_000913/3192818‑3192966 | cTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttacttta > 2:4900241/1‑76 (MQ=255) tAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGtt < 2:5354538/76‑1 (MQ=255) tAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGtt < 2:333509/76‑1 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTg > 2:3674253/1‑76 (MQ=255) aGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGa < 2:4255423/76‑1 (MQ=255) gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaa < 2:3335070/76‑1 (MQ=255) ttCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaa < 1:980202/76‑1 (MQ=255) gCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaa > 2:1535541/1‑75 (MQ=255) gCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaa > 1:4192407/1‑75 (MQ=255) cAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaat > 2:5092274/1‑74 (MQ=255) acTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaac < 2:1474096/75‑1 (MQ=255) attaACAGCCAAGCAGAGGGGGTAGAAATCCTCACTCACCCTTGTGCCTTTACTGTAGGTTGAAAAAAAATCAGCg < 1:4523450/76‑1 (MQ=255) taACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTc > 1:5193488/1‑76 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCa < 2:1206338/76‑1 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTCTTCCTTTACTTTAGGGTGAAAAAAAATCAGCGTCa > 1:379061/1‑76 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa < 2:3204426/76‑1 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa < 1:628586/76‑1 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa < 2:1477759/76‑1 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa > 2:596499/1‑76 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAg > 1:3134629‑M2/1‑75 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGa < 1:2407461/57‑1 (MQ=255) ctcaTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAgg < 1:2832543/73‑1 (MQ=255) ccAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaag < 2:3030849‑M2/76‑5 (MQ=255) ccAATCAGAGGGGGGAGAAATCCTCCCTCACCCTAGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaag < 2:5784741‑M2/76‑5 (MQ=255) cAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaagg > 1:1778994‑M2/1‑71 (MQ=255) caTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGcc > 1:4916035/2‑76 (MQ=255) aaTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggt > 2:2401634‑M2/1‑70 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt < 1:3104190/76‑1 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtt < 1:3678996‑M2/76‑8 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtt > 2:4341182‑M2/1‑69 (MQ=255) tCAGAGGGGGGAGAAATCCTCCGTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:5287526‑M2/1‑68 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:3170311‑M2/1‑68 (MQ=255) tCAGAGGGGGGAGAAAGCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAACAACAACAGCGTCAATAGGCCTg < 2:3266276/76‑1 (MQ=255) gggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTGGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCAt < 2:2259961/75‑1 (MQ=255) ggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATg > 1:723829/1‑76 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaa > 1:2534255‑M2/1‑62 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGCTGAAAAAAAATCAGCGTCAAgaaggtttcattaa < 2:3034630‑M2/76‑15 (MQ=255) ggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAg > 1:3831717/1‑64 (MQ=255) ggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAg < 2:3831716/64‑1 (MQ=255) ggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTa < 2:2534986/76‑1 (MQ=255) ggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaac > 1:4932249‑M2/1‑61 (MQ=255) gggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTAc < 1:4705556/76‑1 (MQ=255) gggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTAc > 1:4531273/1‑76 (MQ=255) ggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCCTGTAca > 2:5801664/1‑75 (MQ=255) ggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACg > 1:67830/1‑76 (MQ=255) ggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacac < 2:1880510‑M2/76‑18 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 1:5174780/1‑76 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 1:3426921/1‑76 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa < 2:4916034/76‑1 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 2:4764667/1‑76 (MQ=255) agaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGaa < 2:4287075/76‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacat < 2:3743578‑M2/76‑20 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg > 2:4586379/1‑76 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg > 1:2855096/1‑76 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacata > 1:4156344‑M2/1‑56 (MQ=255) aaaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatat < 2:1111562‑M2/76‑22 (MQ=255) aTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCga < 1:4624201/76‑1 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag < 2:863545/76‑1 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag > 1:3459508/1‑76 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag < 1:5783773/76‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:1428058‑M2/1‑52 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:5395404‑M2/1‑52 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccct > 1:3674253‑M2/1‑51 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccct > 1:2931899‑M2/1‑51 (MQ=255) tccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATc < 1:3023319/76‑1 (MQ=255) ctcacccTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggccc < 2:2799079‑M2/73‑31 (MQ=255) cTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCtgt > 2:4557132/1‑76 (MQ=255) tgcccTTGTTCCTTTACTCTAGGTTGAACACACAACAGCGTCAATAGGCCTGGCATGTACGAAGCGAGCTCTGTGa < 2:2456506/74‑1 (MQ=255) cacCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaa < 1:3713674‑M2/75‑33 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaa > 2:1794060‑M2/1‑43 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaa > 1:5720996‑M2/1‑43 (MQ=255) cTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCg < 2:1041603/76‑1 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc > 1:2291884/1‑76 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc < 1:1130652/76‑1 (MQ=255) tGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 1:2148297‑M2/1‑38 (MQ=255) tGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 1:3600693‑M2/1‑38 (MQ=255) tGTTCCTTTACTTGAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 2:2756823‑M2/1‑38 (MQ=255) gTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCtt < 2:3307005/76‑1 (MQ=255) gTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCtt < 1:3042614/76‑1 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGTTCTGTGAACCGCttt > 1:1102838/1‑76 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCttt > 2:576091/1‑76 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCttt > 1:3167004/1‑76 (MQ=255) tCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTc < 1:2265399/76‑1 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc > 1:2891251/1‑76 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc > 2:1265150/1‑76 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc < 1:560759/76‑1 (MQ=255) ctttactttaGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccgggga < 2:5350280‑M2/76‑44 (MQ=255) cTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCg > 2:3070443/1‑76 (MQ=255) tttACTTTAGGTTGAAAAAAAATTAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggat < 1:334345‑M2/76‑45 (MQ=255) tagtctAGATTGAAAAATCAACATCGTGAATAGGTCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGtt < 2:2844886/73‑1 (MQ=255) tACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattt > 1:486716‑M2/1‑30 (MQ=255) aCTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggatttt < 2:2599652‑M2/76‑48 (MQ=255) aCTTTAGGTTGAAAAAAAATCAGCGTCAAgaagggttcattaacacatatccctggcccaaaaagccggggatttt < 1:5366616‑M2/76‑48 (MQ=255) aCTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTa < 1:2944884/76‑1 (MQ=255) aCTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTa < 2:3974393/76‑1 (MQ=255) ctctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAg < 1:169012/76‑1 (MQ=255) tctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGc > 2:4410937/1‑76 (MQ=255) tctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGc < 1:5756013/76‑1 (MQ=255) ctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGcc > 2:3851402/1‑76 (MQ=255) ctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGcc > 1:4696964/1‑76 (MQ=255) | CTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCC > NC_000913/3192818‑3192966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |