| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,905 | 0 | C | A | 43.3% | 25.8 / 197.0 | 181 | intergenic (+364/+56) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base C (47/55); new base A (41/37); total (89/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 188 total aligned reads displayed.
GCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTGTT > NC_000913/3192833‑3192980 | acTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaac < 2:1474096/75‑1 (MQ=255) attaACAGCCAAGCAGAGGGGGTAGAAATCCTCACTCACCCTTGTGCCTTTACTGTAGGTTGAAAAAAAATCAGCg < 1:4523450/76‑1 (MQ=255) taACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTc > 1:5193488/1‑76 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa < 2:3204426/76‑1 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAg > 2:4341370‑M2/1‑75 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAg > 1:3134629‑M2/1‑75 (MQ=255) ctcaTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAgg < 1:2832543/73‑1 (MQ=255) ccAATCAGAGGGGGGAGAAATCCTCCCTCACCCTAGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaag < 2:5784741‑M2/76‑5 (MQ=255) tcaTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 1:3670385/74‑1 (MQ=255) caTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGcc < 2:3700423/75‑1 (MQ=255) aaTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggt > 2:2401634‑M2/1‑70 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtt < 1:3678996‑M2/76‑8 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtt > 2:4341182‑M2/1‑69 (MQ=255) tCAGAGGGGGGAGAAATCCTCCGTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:5287526‑M2/1‑68 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTg < 2:3307172/76‑1 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:3170311‑M2/1‑68 (MQ=255) agagGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGcc > 1:3501149/1‑76 (MQ=255) gggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTGGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCAt < 2:2259961/75‑1 (MQ=255) ggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATg > 1:2502287/1‑76 (MQ=255) ggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATg > 1:723829/1‑76 (MQ=255) gggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGt < 1:4041093/76‑1 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGCTGAAAAAAAATCAGCGTCAAgaaggtttcattaa < 2:3034630‑M2/76‑15 (MQ=255) ggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAg > 1:3831717/1‑64 (MQ=255) ggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAg < 2:3831716/64‑1 (MQ=255) ggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaac > 1:4932249‑M2/1‑61 (MQ=255) gggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTAc < 1:4705556/76‑1 (MQ=255) gggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaaca > 2:4801021‑M2/1‑60 (MQ=255) gggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaaca < 1:1813301‑M2/76‑17 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa < 2:4916034/76‑1 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 1:5174780/1‑76 (MQ=255) agaATTCCGCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGaa < 2:2912585/76‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacat < 2:3743578‑M2/76‑20 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTCCTCTCGGTTGAACAACCAACAGCGTCCATAGGCCTGCCCTGTACGCAg > 2:4640467/1‑76 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg < 1:293087/76‑1 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg > 1:2855096/1‑76 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAGAAAAATCAGCGTCAAgaaggtttcattaacacata > 2:5569782‑M2/1‑56 (MQ=255) aaaTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc < 2:32896/76‑1 (MQ=255) aaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatc < 2:5833918‑M2/76‑23 (MQ=255) aTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAACAGGCCTGACATGTACGAAGCga > 2:3958063/1‑76 (MQ=255) aTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatcc > 2:5338075‑M2/1‑53 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag < 2:863545/76‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:5395404‑M2/1‑52 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:1428058‑M2/1‑52 (MQ=255) tccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATc < 1:3023319/76‑1 (MQ=255) cTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCtgt > 2:4557132/1‑76 (MQ=255) tgcccTTGTTCCTTTACTCTAGGTTGAACACACAACAGCGTCAATAGGCCTGGCATGTACGAAGCGAGCTCTGTGa < 2:2456506/74‑1 (MQ=255) cacCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaa > 2:601669‑M2/2‑44 (MQ=255) cacCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaa < 1:3699718‑M2/75‑33 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaa > 1:5720996‑M2/1‑43 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaa > 2:1794060‑M2/1‑43 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatagccctggcccaaa < 1:605556‑M2/76‑34 (MQ=255) cccTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccccggcccaaaa > 1:747551‑M2/1‑42 (MQ=255) ccTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAAcc < 1:3719343/76‑1 (MQ=255) cTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCg < 2:1041603/76‑1 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc > 1:5767624/1‑76 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc > 1:2291884/1‑76 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc < 1:1130652/76‑1 (MQ=255) tGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 1:3026947‑M2/1‑38 (MQ=255) tGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 1:2148297‑M2/1‑38 (MQ=255) tGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc < 1:4625059‑M2/76‑39 (MQ=255) tGTTCCTTTACTTGAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 2:2756823‑M2/1‑38 (MQ=255) tttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCtt < 1:3080145/75‑1 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCttt > 2:1132893/1‑76 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCttt > 2:576091/1‑76 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCttt > 1:3167004/1‑76 (MQ=255) ccTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccgggg > 2:1221488‑M2/1‑34 (MQ=255) ccTTTACTCTAGGTTTAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc < 1:660163/76‑1 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc < 1:560759/76‑1 (MQ=255) cTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCg > 2:3070443/1‑76 (MQ=255) tttACTTTAGGTTGAAAAAAAATTAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggat < 1:334345‑M2/76‑45 (MQ=255) ttACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGt < 1:4882153/76‑1 (MQ=255) tagtctAGATTGAAAAATCAACATCGTGAATAGGTCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGtt < 2:2844886/73‑1 (MQ=255) tACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattt < 1:4325398‑M2/76‑47 (MQ=255) tACTCTAGGATGAAAAATCAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGtt < 2:2824950/76‑1 (MQ=255) aCTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggatttt < 2:2599652‑M2/76‑48 (MQ=255) aCTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTa < 1:2944884/76‑1 (MQ=255) aCTCTAGATAGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTa < 1:2583254/76‑1 (MQ=255) ctctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAg < 1:169012/76‑1 (MQ=255) tttagGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggatttttt < 2:2572542‑M2/74‑50 (MQ=255) tctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGc > 2:4410937/1‑76 (MQ=255) ttaGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggatttttta > 2:3699718‑M2/2‑26 (MQ=255) ctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGcc < 1:3427370/76‑1 (MQ=255) tAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCt > 2:4160032/1‑76 (MQ=255) tAGGTTGAAAAAACAACAGCGTCAATAGGCACGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCt > 1:4218319/1‑76 (MQ=255) aGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtt < 1:5471062/76‑1 (MQ=255) aGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtt > 1:2282112/1‑76 (MQ=255) aGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtt > 2:151420/1‑76 (MQ=255) ggTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCttt < 1:4586380/76‑1 (MQ=255) ttGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatct > 1:5350281‑M2/1‑21 (MQ=255) ttGAAAAAAAATCAGCGGCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatct > 2:5022103‑M2/1‑21 (MQ=255) tGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtttttt > 1:1100706/1‑76 (MQ=255) tGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtttttt > 1:101975/1‑76 (MQ=255) tGAAAAAAAATCAGCGTCAAgaaggtttcattaacacctatccctggcccacaaagccggggcttttttatatctg < 1:4562215‑M2/76‑57 (MQ=255) gAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatctgc < 2:879340‑M2/76‑58 (MQ=255) aaaaaaCAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTAt < 1:2161699/76‑1 (MQ=255) aaaaaaATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatctgcgt > 2:4298626‑M2/1‑17 (MQ=255) aaaaCAACAGCGTCAATAGGCCTGCCATGTCCGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATcc < 2:4063540/76‑1 (MQ=255) aaaCAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCt > 2:1654420/1‑76 (MQ=255) aacaacAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTg > 2:1267933/1‑76 (MQ=255) caacaGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTGtt < 1:4607177/76‑1 (MQ=255) | GCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTGTT > NC_000913/3192833‑3192980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |