| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,192,907 | 0 | A | T | 44.3% | 58.4 / 222.8 | 183 | intergenic (+366/+54) | b3051/b3052 | putative membrane protein/putative kinase |
| Reads supporting (aligned to +/- strand): ref base A (47/55); new base T (43/38); total (90/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 191 total aligned reads displayed.
GCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTGTTGG > NC_000913/3192833‑3192982 | acTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaac < 2:1474096/75‑1 (MQ=255) taACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTc > 1:5193488/1‑76 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCa < 2:1206338/76‑1 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTCTTCCTTTACTTTAGGGTGAAAAAAAATCAGCGTCa > 1:379061/1‑76 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaa < 2:3204426/76‑1 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAg > 2:4341370‑M2/1‑75 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAga > 2:147241‑M2/1‑74 (MQ=255) ctcaTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAgg < 1:2832543/73‑1 (MQ=255) ccAATCAGAGGGGGGAGAAATCCTCCCTCACCCTAGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaag < 2:5784741‑M2/76‑5 (MQ=255) tcaTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 1:3670385/74‑1 (MQ=255) caTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGcc > 1:4916035/2‑76 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt < 1:3104190/76‑1 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt > 2:2347034/1‑76 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtt < 1:3678996‑M2/76‑8 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTg < 2:3307172/76‑1 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggttt > 2:3170311‑M2/1‑68 (MQ=255) agagGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGcc > 1:3501149/1‑76 (MQ=255) ggggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATg > 1:723829/1‑76 (MQ=255) ggggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcatta > 1:5604813‑M2/1‑63 (MQ=255) gggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGt < 2:3806908/76‑1 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaa > 1:2534255‑M2/1‑62 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGCTGAAAAAAAATCAGCGTCAAgaaggtttcattaa < 2:3034630‑M2/76‑15 (MQ=255) ggggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTa < 2:2534986/76‑1 (MQ=255) gggAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTAc > 1:4531273/1‑76 (MQ=255) gggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaaca > 2:4801021‑M2/1‑60 (MQ=255) gggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaaca < 1:1813301‑M2/76‑17 (MQ=255) ggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacac < 2:1880510‑M2/76‑18 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 1:3426921/1‑76 (MQ=255) gagaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 1:5174780/1‑76 (MQ=255) agaATTCCGCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGaa < 2:2912585/76‑1 (MQ=255) agaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGaa < 2:4287075/76‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacat < 2:3743578‑M2/76‑20 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTCCTCTCGGTTGAACAACCAACAGCGTCCATAGGCCTGCCCTGTACGCAg > 2:4640467/1‑76 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg < 1:293087/76‑1 (MQ=255) gaAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg > 1:2855096/1‑76 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAGAAAAATCAGCGTCAAgaaggtttcattaacacata > 2:5569782‑M2/1‑56 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacata > 1:4156344‑M2/1‑56 (MQ=255) aaaTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc < 2:32896/76‑1 (MQ=255) aaaTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc < 1:3138256/76‑1 (MQ=255) aaaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatat < 2:1111562‑M2/76‑22 (MQ=255) aaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatc < 2:5833918‑M2/76‑23 (MQ=255) aTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCga > 2:4926111/1‑76 (MQ=255) aTCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCga < 1:4624201/76‑1 (MQ=255) aTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatcc > 2:5338075‑M2/1‑53 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag > 1:3459508/1‑76 (MQ=255) tcctccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCgag < 2:863545/76‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccc > 1:1428058‑M2/1‑52 (MQ=255) ctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatacccctg < 1:996683‑M2/76‑27 (MQ=255) tccCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATc < 1:3023319/76‑1 (MQ=255) cccTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggc < 2:5263049‑M2/76‑29 (MQ=255) cTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCtgt > 2:4557132/1‑76 (MQ=255) cacCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaa < 1:3713674‑M2/75‑33 (MQ=255) cacCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaa > 2:601669‑M2/2‑44 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaa > 1:5720996‑M2/1‑43 (MQ=255) aCCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatagccctggcccaaa < 1:605556‑M2/76‑34 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc > 1:5767624/1‑76 (MQ=255) ttGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGc < 1:1130652/76‑1 (MQ=255) tGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 1:2148297‑M2/1‑38 (MQ=255) tGTTCCTTTACTTGAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagcc > 2:2756823‑M2/1‑38 (MQ=255) gTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccg > 2:4306182‑M2/1‑37 (MQ=255) gTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCtt < 1:3042614/76‑1 (MQ=255) ttCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCttt > 2:576091/1‑76 (MQ=255) tCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTc > 1:499779/1‑76 (MQ=255) tCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTc < 1:2314510/76‑1 (MQ=255) ccTTTACTCTAGGTTTAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc < 1:660163/76‑1 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc > 2:1265150/1‑76 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc < 1:560759/76‑1 (MQ=255) ccTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTcc < 1:3317572/76‑1 (MQ=255) cTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCg < 2:2592208/76‑1 (MQ=255) cTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCg > 2:4069116/1‑76 (MQ=255) cTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCg > 2:3070443/1‑76 (MQ=255) tttACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggat > 1:4596145‑M2/1‑32 (MQ=255) tagtctAGATTGAAAAATCAACATCGTGAATAGGTCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGtt < 2:2844886/73‑1 (MQ=255) tACTTTAGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattt > 1:486716‑M2/1‑30 (MQ=255) tACTCTAGGATGAAAAATCAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGtt < 2:2824950/76‑1 (MQ=255) aCTTTAGGTTGAAAAAAAATCAGCGTCAAgaagggttcattaacacatatccctggcccaaaaagccggggatttt < 1:5366616‑M2/76‑48 (MQ=255) aCTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTa < 1:2944884/76‑1 (MQ=255) aCTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTa < 2:3974393/76‑1 (MQ=255) ctctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAg < 1:169012/76‑1 (MQ=255) tctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGc > 2:4410937/1‑76 (MQ=255) ttaGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggatttttta > 2:3699718‑M2/2‑26 (MQ=255) ctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGcc > 2:3851402/1‑76 (MQ=255) ctAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGcc > 1:4696964/1‑76 (MQ=255) aGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtt > 2:151420/1‑76 (MQ=255) aGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtt > 1:2282112/1‑76 (MQ=255) aGGTTGAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttata < 1:3852793‑M2/76‑53 (MQ=255) ggTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCttt < 1:4586380/76‑1 (MQ=255) ttGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCttttt > 1:446200/1‑76 (MQ=255) tGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtttttt > 1:1100706/1‑76 (MQ=255) tGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtttttt < 1:4308591/76‑1 (MQ=255) tGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCtttttt > 1:101975/1‑76 (MQ=255) gAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTa > 2:2616610/1‑76 (MQ=255) gAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatctgc > 2:4872495‑M2/1‑19 (MQ=255) gAAAAAAAATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatctgc < 2:879340‑M2/76‑58 (MQ=255) gAAAAAA‑ATCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatctgcg > 1:2659680‑M2/1‑18 (MQ=255) aaaaaaaaTCAGCGTCAAgaaggtttcattaacacatatccctggcccaaaaagccggggattttttatatctgcg < 1:5464455‑M2/76‑59 (MQ=255) aaaaaaCAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTAt < 1:2161699/76‑1 (MQ=255) aaaaaCAACAGCGTCAATAGGCCTGCCATGGACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATc > 2:2425069/1‑76 (MQ=255) aaaCAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCt > 2:1654420/1‑76 (MQ=255) acaGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTGTTgg < 2:3707132/76‑1 (MQ=255) | GCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGCGAGATCTGTGAACCGCTTTCCGGTTAGCCTTTTTTATCCTGTTGG > NC_000913/3192833‑3192982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |