| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,528 | T→C | Q8R (CAG→CGG) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,528 | 0 | T | C | 99.2% | 315.5 / NA | 122 | Q8R (CAG→CGG) | araD | L‑ribulose‑5‑phosphate 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (53/68); minor base A (1/0); total (54/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 122 total aligned reads displayed.
ACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAT > NC_000913/66456‑66598 | cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCg < 1:3014204/76‑1 (MQ=255) cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCg < 1:5501261/76‑1 (MQ=255) cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCg < 1:2485334/76‑1 (MQ=255) ggCGCTGACGTTGCTCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATCCCCGGCGc < 2:3028798‑M1/76‑2 (MQ=255) ggCGCTGACGTTGCCCCATGTGAGCGTGACCAGGCTGCGTTTTGGCAGCGCCAGGCTTGCTTCTAATACCCGGCGc > 1:2208164‑M1/1‑75 (MQ=255) gcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg > 1:3892417‑M1/1‑72 (MQ=255) gcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg > 2:4959116‑M1/1‑72 (MQ=255) tggcgtgGCCCCAGGTGGGGGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag < 2:437733‑M1/73‑7 (MQ=255) tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag > 1:1165233‑M1/1‑70 (MQ=255) tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag < 1:5094729‑M1/76‑7 (MQ=255) tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag > 1:2889545‑M1/1‑70 (MQ=255) tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag < 1:2483906‑M1/76‑7 (MQ=255) ggaCGTTGCCCCATGTGAGCGGGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag < 2:4675967‑M1/75‑7 (MQ=255) gACGGTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgaga < 2:481402‑M1/76‑8 (MQ=255) gTTGCCCCATGTGAGCGTGACCAGGTTGTGCTTTGGCAGCGCCAGGTTGGCTTCTAACACCCGGCGcccgagactc > 2:5359760‑M1/1‑66 (MQ=255) tGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACTCGGCGcccgagacccgc < 1:795946‑M1/76‑13 (MQ=255) tGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgggacccgc > 1:2425997‑M1/1‑64 (MQ=255) gCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgca < 2:5302002‑M1/76‑14 (MQ=255) ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac > 1:816979‑M1/1‑62 (MQ=255) ccccATGGGAGGGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac < 2:2381787‑M1/76‑15 (MQ=255) cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacg < 1:1178227‑M1/76‑16 (MQ=255) ccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgc > 1:4631941‑M1/1‑60 (MQ=255) cATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcg > 1:5668712‑M1/1‑59 (MQ=255) cATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcg > 1:3763153‑M1/1‑59 (MQ=255) aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgt < 1:2928034‑M1/76‑19 (MQ=255) aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgt < 1:3910474‑M1/76‑19 (MQ=255) tgtgAGCGTGCCCAGGTTGTGTTTTGGCAGCTCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 2:1277461‑M1/76‑20 (MQ=255) tgtgAGCGTGCCCAGGTTGTGTTGTGGCAGCGCCAGGTTTGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 2:1380357‑M1/76‑20 (MQ=255) tgtgAGCGTGACCAGTTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 1:4837659‑M1/76‑20 (MQ=255) tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 1:3562364‑M1/76‑20 (MQ=255) tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 2:4849683‑M1/76‑20 (MQ=255) tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 1:4310820‑M1/76‑20 (MQ=255) gttgagCGTGGCAAGGTTGTGTTTGGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc < 2:5257039‑M1/74‑20 (MQ=255) gggagCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcg > 1:4956614‑M1/3‑56 (MQ=255) tgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcctcgc > 2:3688109‑M1/1‑55 (MQ=255) gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgac > 1:5364256‑M1/1‑54 (MQ=255) gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgac > 1:4774055‑M1/1‑54 (MQ=255) aGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc < 2:2371541‑M1/76‑24 (MQ=255) aGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc > 1:433543‑M1/1‑53 (MQ=255) aGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc > 1:2875688‑M1/1‑53 (MQ=255) aGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc > 2:4901233‑M1/1‑53 (MQ=255) cGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgccctg > 2:3669439‑M1/1‑51 (MQ=255) tGACCAGGTTGTGTTTTGTCAGCGCCAGGTTGGCTTCTAATACCCGTCGcccgagacccgcactcgtcgacctgca < 1:4217316‑M1/76‑28 (MQ=255) gACGAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcag < 2:640387‑M1/76‑29 (MQ=255) gACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATGCCCGGCGcccgagacccgcacgcgtcgacctgcag < 2:3618932‑M1/76‑29 (MQ=255) gACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgccctgcag > 2:4480716‑M1/1‑48 (MQ=255) aCCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcagg > 1:3563660‑M1/1‑47 (MQ=255) ccAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt < 1:3510774‑M1/76‑31 (MQ=255) cAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggta < 1:2355202‑M1/76‑32 (MQ=255) cAGGTGGTGTTTGGGCAGCGCCAGGTGGGCTTCTAAGACCCGGCGcccgagacccgcacgcgtcgacctgcaggta < 2:1406929‑M1/76‑32 (MQ=255) gtttgtGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatg < 1:463770‑M1/74‑34 (MQ=255) ttGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatgga < 1:3062186‑M1/76‑36 (MQ=255) ttGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatgga > 2:2545220‑M1/1‑41 (MQ=255) ttGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatgga < 2:3035032‑M1/76‑36 (MQ=255) gtgtgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgttgacctgcaggtatgga < 2:1200023‑M1/75‑36 (MQ=255) tgtttggCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaa < 2:1637994‑M1/74‑40 (MQ=255) tggggcggcgccAGGTTGGGTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca < 2:1600504‑M1/73‑42 (MQ=255) ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgccggtatggagaaacag > 2:2432892‑M1/1‑34 (MQ=255) ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacccgccggtatcgagaaacag > 2:2411157‑M1/1‑34 (MQ=255) tGGGAGCGCCAGGTTGGCTTGTAAGACCCGGCGgccgagacccgcacgcgtcgacctgcaggtatggagaaacagt < 2:5764116‑M1/76‑44 (MQ=255) tGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagt > 1:4794360‑M1/1‑33 (MQ=255) ggCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagta < 1:3581665‑M1/76‑45 (MQ=255) cAGCGCGAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgccctgcaggtatggagaaacagtaga < 1:926805‑M1/76‑47 (MQ=255) aGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagag < 2:2980262‑M1/76‑48 (MQ=255) gcgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagaga < 2:4435732‑M1/76‑49 (MQ=255) gcgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagaga > 2:3278442‑M1/1‑28 (MQ=255) cgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagag > 1:2012565‑M1/1‑27 (MQ=255) gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt > 1:5060271‑M1/1‑26 (MQ=255) gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt > 2:2805343‑M1/1‑26 (MQ=255) gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt > 1:1967413‑M1/1‑26 (MQ=255) ccgggttgGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagtt < 2:3854488‑M1/73‑52 (MQ=255) ccgggttgGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagtt < 2:1226786‑M1/73‑52 (MQ=255) ccAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagtt < 1:3051444‑M1/76‑52 (MQ=255) ccAGGTTGGCTTCGAATGCGCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagtt < 1:5707236‑M1/76‑52 (MQ=255) ggTTGGCTTCTAATCCCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcg < 2:3446038‑M1/76‑55 (MQ=255) ggTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcg > 1:1666712‑M1/1‑22 (MQ=255) tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgccctgcaggtatggagaaacagtagagagttgcgata > 2:2119226‑M1/1‑19 (MQ=255) tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata < 1:2477603‑M1/76‑58 (MQ=255) tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata > 2:5205471‑M1/1‑19 (MQ=255) tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata > 2:549879‑M1/1‑19 (MQ=255) tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata < 1:5196476‑M1/76‑58 (MQ=255) ggCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataa < 2:2954501‑M1/76‑59 (MQ=255) ggCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataa < 2:2425997‑M1/76‑59 (MQ=255) gCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaa > 1:2854890‑M1/1‑17 (MQ=255) cTTCTAATACCCTGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaa < 2:2088710‑M1/76‑61 (MQ=255) cTTCTAATACCCGGCGcccgagacccgcacgcgtcgccctgcaggtatggagaaacagtagagagttgcgataaaa > 2:605976‑M1/1‑16 (MQ=255) ttCTAATACCCGGCGcccgaggcccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa < 1:3796220‑M1/76‑62 (MQ=255) ttCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagacacagcagagagtggctataaaaa > 2:5736461‑M1/1‑15 (MQ=255) ttCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa > 1:3170211‑M1/1‑15 (MQ=255) ttCTAATACCAGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa > 1:5483693‑M1/1‑15 (MQ=255) tCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaag < 1:348320‑M1/76‑63 (MQ=255) tCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaag > 1:1757348‑M1/1‑14 (MQ=255) tCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaag > 1:4786392‑M1/1‑14 (MQ=255) cgaataCCCGGCGcgcgagacccgctcgcgtcgccctgcaggtatggagaaacggtagagagttgcgataaaaagc < 2:3568088‑M1/74‑64 (MQ=255) cTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgttaaaaagc < 1:250066‑M1/76‑64 (MQ=255) cTAATACCCGGCGcccgagacccgcacgcgtcgaccggcaggtatggagaaacagtagagagttgcgataaaaagc < 2:4448511‑M1/76‑64 (MQ=255) tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg > 1:2646939‑M1/1‑12 (MQ=255) aaTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgt < 1:5007246‑M1/76‑66 (MQ=255) aTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtc > 1:5459011‑M1/1‑10 (MQ=255) aTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtc > 1:1091800‑M1/1‑10 (MQ=255) | ACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAT > NC_000913/66456‑66598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |