BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,754,310	C→A	R382M (AGG→ATG)	yiaY ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,754,310	0	C	A	100.0%	391.6 / NA	131	R382M (AGG→ATG)	yiaY	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (77/54); total (77/54)

Only 100 of 132 total aligned reads displayed.

TTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGC‑‑‑ATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATC  >  NC_000913/3754236‑3754377

ttACGGGCAAGGCTTACAGAGATACTTGAAAAAGCGCCTAACAATTTGCCACCCGTTCACGAAATGAAGATCAccc                                                                       >  2:5415265/1‑75 (MQ=255)

 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATc                                                                      >  1:4913575/1‑76 (MQ=255)

  aCGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                                                     <  1:3993377/76‑1 (MQ=255)

  aCGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                                                     >  1:1622464/1‑76 (MQ=255)

   cGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                    >  2:1257308/1‑76 (MQ=255)

    gggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCt                                                                   >  1:3962164/1‑76 (MQ=255)

     ggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                  >  1:2447782/1‑76 (MQ=255)

     ggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATCTGCCACCCGTTGTGGAAATGATGATTACATAGCTg                                                                  >  2:2837961/1‑76 (MQ=255)

      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                 >  1:2319395/1‑76 (MQ=255)

      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                 >  1:4645003/1‑76 (MQ=255)

       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                >  1:2777726‑M1/1‑75 (MQ=255)

        aaGGGTTACAGAGCTAGTTGCAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                               <  1:2984666‑M1/76‑3 (MQ=255)

        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                               >  2:5201428‑M1/1‑74 (MQ=255)

         aGGGTTACAGGGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcg                                                              <  1:4278198‑M1/76‑4 (MQ=255)

         aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcg                                                              >  2:4826690‑M1/1‑73 (MQ=255)

          gggTTGCAGAGATAGTTGAAAAAGCGGCTAGCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCtcga                                                             <  2:687698‑M1/76‑5 (MQ=255)

          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcga                                                             >  2:2489785‑M1/1‑72 (MQ=255)

          gggTTACAGAGATAGGTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcga                                                             >  2:3862883‑M1/1‑72 (MQ=255)

           ggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                            >  1:180323‑M1/1‑71 (MQ=255)

            gTTACAGAGATAGTTGAAAAAGCCGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgata                                                           >  2:3368933‑M1/1‑70 (MQ=255)

             ttACAGAGATAGTGGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAAGGATGATTACATCGCTGCgcgataa                                                          <  2:2040934‑M1/76‑8 (MQ=255)

              tACAGAGATAGTTGAAAAAGAGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                         <  2:4044724‑M1/76‑9 (MQ=255)

               gcaGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaat                                                        >  2:2640004‑M1/2‑67 (MQ=255)

               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaac                                                        >  2:2481323‑M1/1‑67 (MQ=255)

                cAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                       >  2:1068076‑M1/1‑66 (MQ=255)

                aagagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                       <  2:5041877‑M1/75‑11 (MQ=255)

                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                     <  1:4954303‑M1/76‑13 (MQ=255)

                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                     <  1:60149‑M1/76‑13 (MQ=255)

                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgcca                                                   >  2:3297603‑M1/1‑62 (MQ=255)

                     aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgatagatcgccac                                                  >  1:793636‑M1/1‑61 (MQ=255)

                     aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccac                                                  <  1:2735607‑M1/76‑16 (MQ=255)

                     aTAGTGGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccac                                                  <  2:4064473‑M1/76‑16 (MQ=255)

                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccaca                                                 <  2:2391507‑M1/76‑17 (MQ=255)

                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccaca                                                 >  2:4331605‑M1/1‑60 (MQ=255)

                        gTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                               >  2:4514239‑M1/1‑58 (MQ=255)

                         ttGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                              >  2:5261778‑M1/1‑57 (MQ=255)

                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                           <  1:1787796‑M1/76‑23 (MQ=255)

                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                           >  1:3841905‑M1/1‑54 (MQ=255)

                             aaatGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                          <  1:1377026‑M1/76‑24 (MQ=255)

                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCCCTGCgcgacaaatcgccacactttcttc                                         >  2:5267044‑M1/1‑52 (MQ=255)

                                aGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                       >  1:182019‑M1/1‑50 (MQ=255)

                                 gCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtg                                      <  1:1476943‑M1/76‑28 (MQ=255)

                                 gCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtg                                      <  1:5261779‑M1/76‑28 (MQ=255)

                                  cGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                     >  2:3846156‑M1/1‑48 (MQ=255)

                                    gCTAACACTTTGCCAGCCGCTGAGGAAATGATGATTACATCCCTGCGCGATAACACGCCACAATCTCTTCGCGAGt                                   >  2:5045408/1‑76 (MQ=255)

                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagt                                   >  1:4124125‑M1/1‑46 (MQ=255)

                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                  <  2:2319395‑M1/76‑32 (MQ=255)

                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                  >  2:3981118‑M1/1‑45 (MQ=255)

                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttagtgagtt                                  >  2:1167178‑M1/1‑45 (MQ=255)

                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttg                                 <  1:5674117‑M1/76‑33 (MQ=255)

                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttg                                 >  1:2471867‑M1/1‑44 (MQ=255)

                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttg                                 >  2:2919157‑M1/1‑44 (MQ=255)

                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcatcacaatttattcgtgagttg                                 >  1:3352925‑M1/1‑44 (MQ=255)

                                      tAACAATTTGCCAGCCGTTGCGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttg                                 >  1:3410038‑M1/1‑44 (MQ=255)

                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                >  2:2471451‑M1/1‑43 (MQ=255)

                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                >  1:1027566‑M1/1‑43 (MQ=255)

                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                <  2:501846‑M1/76‑34 (MQ=255)

                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                >  2:4702347‑M1/1‑43 (MQ=255)

                                       aaCAATTGGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                <  2:3430509‑M1/76‑34 (MQ=255)

                                         cAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcct                              >  2:4538238‑M1/1‑41 (MQ=255)

                                          aaTTTGCGAGCCGTTGTGGAAATGATGATTCCATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                             <  2:300795‑M1/76‑37 (MQ=255)

                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                             >  2:5792078‑M1/1‑40 (MQ=255)

                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                             >  1:1028341‑M1/1‑40 (MQ=255)

                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                             >  1:2228531‑M1/1‑40 (MQ=255)

                                           aTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcctgagttgcctgg                            >  2:21029‑M1/1‑39 (MQ=255)

                                            tttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctgga                           >  1:3288634‑M1/1‑38 (MQ=255)

                                             ttGCCGGCCGTTGTGGAAATGATGTTTACATCGCTGCgcgataaatcgccacaatttcttcgtgatttgcctggat                          <  2:3127107‑M1/76‑40 (MQ=255)

                                             ttGCCAGCCGTTGTGGGAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgaggtgcctggat                          >  1:3229996‑M1/1‑37 (MQ=255)

                                             ttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                          >  2:423216‑M1/1‑37 (MQ=255)

                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgctataaatcgccccaatttcttcgtgagttgcctggctc                         <  1:3493101‑M1/76‑41 (MQ=255)

                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                         <  1:1547176‑M1/76‑41 (MQ=255)

                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                        >  1:4467334‑M1/1‑35 (MQ=255)

                                               gCCAGCAGGTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                        >  1:4308636‑M1/1‑35 (MQ=255)

                                                ccAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                       <  1:2182110‑M1/76‑43 (MQ=255)

                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcggg                      >  1:3770560‑M1/1‑33 (MQ=255)

                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcggg                      >  1:2072872‑M1/1‑33 (MQ=255)

                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggt                     <  1:3663461‑M1/76‑45 (MQ=255)

                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccagaatttcttcgtgagttgcctggatcgggtt                    >  2:4280492‑M1/1‑31 (MQ=255)

                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtt                    <  2:5732661‑M1/76‑46 (MQ=255)

                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtt                    <  2:2716830‑M1/76‑46 (MQ=255)

                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                  >  1:4054276‑M1/1‑29 (MQ=255)

                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagt                 <  2:4556994‑M1/76‑49 (MQ=255)

                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                <  1:3862884‑M1/76‑50 (MQ=255)

                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                >  1:1100374‑M1/1‑27 (MQ=255)

                                                        tgtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa               >  2:2321625‑M1/1‑26 (MQ=255)

                                                         ggggaaATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaa              <  2:946276‑M1/74‑52 (MQ=255)

                                                           ggAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagc            >  2:3973417‑M1/1‑23 (MQ=255)

                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcc           >  1:4719138‑M1/1‑22 (MQ=255)

                                                              aaTGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccac         <  1:4826691‑M1/76‑57 (MQ=255)

                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca        <  1:5714162‑M1/76‑58 (MQ=255)

                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                       >  1:5632599‑M1/1‑19 (MQ=255)

                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                       <  2:5632597‑M1/45‑27 (MQ=255)

                                                                tgatgaTTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag       <  1:3529227‑M1/76‑59 (MQ=255)

                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggc     <  2:3835667‑M1/76‑61 (MQ=255)

                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat   >  2:5629008‑M1/1‑14 (MQ=255)

                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat   >  2:1369390‑M1/1‑14 (MQ=255)

                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat   <  2:149556‑M1/76‑63 (MQ=255)

                                                                     atTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc  <  1:4140744‑M1/76‑64 (MQ=255)

                                                                     atTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc  <  1:4007968‑M1/76‑64 (MQ=255)

                                                                     atTACATCGCTGCgcgataaatcgccacaatttcctcgtgaggtgcctggatcgggttagtaaagccacaggcatc  >  2:2014749‑M1/1‑13 (MQ=255)

TTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGC‑‑‑ATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATC  >  NC_000913/3754236‑3754377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`