BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,782	A→G	N2S (AAC→AGC)	oppD →	OppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,782	0	A	G	100.0%	419.6 / NA	154	N2S (AAC→AGC)	oppD	OppD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (71/83); total (71/83)

Only 100 of 154 total aligned reads displayed.

TTTCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAACGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGATTTGCG  >  NC_000913/1302712‑1302854

 ttCACCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAGGGAGTGCAGCCATGAGCGTAAt                                                                    <  1:5309383/76‑1 (MQ=255)

 ttCAACTTTATCGGCGATGGATTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGGAAt                                                                    >  1:2293126/1‑76 (MQ=255)

  tCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAAtt                                                                   <  1:4304491/76‑1 (MQ=255)

   cAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTg                                                                  >  1:5269887/1‑76 (MQ=255)

    aaCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGa                                                                 >  2:2823294/1‑76 (MQ=255)

     aCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGaa                                                                >  2:4635465/1‑76 (MQ=255)

     aCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGaa                                                                <  1:2411272/76‑1 (MQ=255)

      cTTTATCGGCGATGGCTTGCGCGATGCCCTCGACCCGAAACATCGTTAAGGAGGGCTGCCATGAGCGGAATTGaaa                                                               >  2:783668/1‑76 (MQ=255)

        ttATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACt                                                             >  1:2060950/1‑76 (MQ=255)

           tCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCa                                                          <  2:4971988/76‑1 (MQ=255)

              gCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACt                                                       >  1:2850504/1‑76 (MQ=255)

               cGATGGCTTGCGTGATGCCCTCGACCCGCAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtg                                                      >  1:3539674/1‑76 (MQ=255)

               cGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtg                                                      >  1:3535898/1‑76 (MQ=255)

               cGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtg                                                      >  1:4169541/1‑76 (MQ=255)

               cGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtg                                                      <  1:2665354/76‑1 (MQ=255)

                gATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgt                                                     >  1:5807663/1‑76 (MQ=255)

                gATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgt                                                     >  1:3254628/1‑76 (MQ=255)

                gATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgt                                                     >  2:2947747/1‑76 (MQ=255)

                gATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgt                                                     >  2:311237/1‑76 (MQ=255)

                 aTGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCCACtgcg                                                    >  2:5452082/1‑74 (MQ=255)

                 aTGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgtg                                                    <  2:3802522/76‑1 (MQ=255)

                  tgacTTGCGTGATGCCCTCGAGCAAAATGATCGTTATGGACTGCATCTATGTGCTTAATTGAAACTGCAGCTATGc                                                   <  1:3971776/73‑1 (MQ=255)

                  tGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGc                                                   <  1:3023246/76‑1 (MQ=255)

                  tGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGc                                                   <  1:2512610/76‑1 (MQ=255)

                   ggCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGcc                                                  <  1:731255/76‑1 (MQ=255)

                    ggttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCg                                                 <  2:1157300/74‑1 (MQ=255)

                    gCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCg                                                 <  1:5264961/76‑1 (MQ=255)

                      ttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCt                                               >  1:504209/1‑76 (MQ=255)

                      ttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCt                                               >  1:5145657/1‑76 (MQ=255)

                       tGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTc                                              <  2:3715995/76‑1 (MQ=255)

                       tGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTc                                              <  1:3037093/76‑1 (MQ=255)

                       tGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTc                                              >  2:769760/1‑76 (MQ=255)

                         cGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGc                                            >  2:1934348/1‑76 (MQ=255)

                         cGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGc                                            >  1:812322/1‑76 (MQ=255)

                          gTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCACCTGTGCCGCTCGca                                           >  2:2709464/1‑76 (MQ=255)

                          gTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcc                                           >  2:856615/1‑75 (MQ=255)

                          gTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGca                                           >  2:3940544/1‑76 (MQ=255)

                           tGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcac                                          <  2:3534856/76‑1 (MQ=255)

                           tGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcac                                          >  2:5549190/1‑76 (MQ=255)

                            gATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgtg                                                    <  1:2478502/65‑1 (MQ=255)

                            gATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgtg                                                    >  2:2478502/1‑65 (MQ=255)

                            gATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcaca                                         >  1:5395226/1‑76 (MQ=255)

                            gATGCCCGCGACGCGAGAGCTCGTGAAGGCGTGCAGCCATGAGCGTAATTGGACCTGCAACTGTGCCGCTCGcaca                                         <  2:1988778/76‑1 (MQ=255)

                              tccccTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaac                                       <  1:192468/74‑1 (MQ=255)

                              tGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaac                                       >  1:3229730/1‑76 (MQ=255)

                               gCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaaca                                      <  2:481106/76‑1 (MQ=255)

                               gCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaaca                                      >  2:4378163/1‑76 (MQ=255)

                                cccGCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaa                                     <  2:4722105/76‑1 (MQ=255)

                                 ccTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaac                                    <  1:4658404/76‑1 (MQ=255)

                                  cTCGACCCGAAAGATCGTTAGGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaaca                                   <  2:336601/76‑1 (MQ=255)

                                  cTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaaca                                   <  1:1646076/76‑1 (MQ=255)

                                  cTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaaca                                   >  1:1713305/1‑76 (MQ=255)

                                   tCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAAGAACAg                                  <  1:2075608/76‑1 (MQ=255)

                                   tCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAg                                  <  2:4160133/76‑1 (MQ=255)

                                     gccccAAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAATCTGCATCTGTGCCGCTCGCACAACAACAGGc                                <  2:4547573/74‑1 (MQ=255)

                                      aCCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCt                               <  1:959912/76‑1 (MQ=255)

                                      aCCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGGAATTGAAAATGCAACTGTGCCGCGCGCACAACAACAGGcc                               >  2:5086551/1‑75 (MQ=255)

                                       cccGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTg                              <  2:3698131/76‑1 (MQ=255)

                                         cGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGAc                            <  2:1589272/76‑1 (MQ=255)

                                          gAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACg                           >  1:958912/1‑76 (MQ=255)

                                          gAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACg                           >  2:3387753/1‑76 (MQ=255)

                                           aaaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGc                          <  1:69189/76‑1 (MQ=255)

                                            aaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAACCTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                         <  2:1067218/76‑1 (MQ=255)

                                            aaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                         <  1:100360/76‑1 (MQ=255)

                                             aGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAc                        <  2:4309969/76‑1 (MQ=255)

                                             aGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAc                        <  1:4132581/76‑1 (MQ=255)

                                              gATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAct                       <  1:3387753/76‑1 (MQ=255)

                                               aTCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActg                      <  2:2679809/76‑1 (MQ=255)

                                               aTCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActg                      <  2:812322/76‑1 (MQ=255)

                                               aTCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActg                      >  1:4430277/1‑76 (MQ=255)

                                               aTCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAAATGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAcag                      >  1:5467452/1‑74 (MQ=255)

                                                tCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAttgc                     >  1:4743859/1‑76 (MQ=255)

                                                tCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgc                     <  2:4128099/76‑1 (MQ=255)

                                                gcGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgc                     <  2:3048024/75‑1 (MQ=255)

                                                  ggtAAGGGGGGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctg                   <  2:246591/74‑1 (MQ=255)

                                                  gTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctg                   >  1:3029872/1‑76 (MQ=255)

                                                  gTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctg                   <  1:1418171/76‑1 (MQ=255)

                                                   ttAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGa                  <  1:3814094/76‑1 (MQ=255)

                                                   ttAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGa                  <  1:4377719/76‑1 (MQ=255)

                                                    tAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAGCAACAGGCTGACGCACTGCTGaa                 <  1:4510901/76‑1 (MQ=255)

                                                    tAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctgca                 >  2:5588825/1‑74 (MQ=255)

                                                    tAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGaa                 <  1:5314001/76‑1 (MQ=255)

                                                     aaGGAGTGCAGCCATGAGCGTAATTGACACTGCAACTGTGACGCTCGCACAACACCAGGCTCACGCACTGCTGCAc                >  1:1424022/1‑76 (MQ=255)

                                                     aaGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAAc                <  1:311237/76‑1 (MQ=255)

                                                     aaGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAAc                >  2:1390622/1‑76 (MQ=255)

                                                     aaGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAAc                >  1:1858935/1‑76 (MQ=255)

                                                     aaGGAGCGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAAc                >  1:1391573/1‑76 (MQ=255)

                                                      aGGAGTGCAGCCATGAGCGTAATTGAAGCTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACg               <  2:2392849/76‑1 (MQ=255)

                                                      aGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACg               <  2:1815271/76‑1 (MQ=255)

                                                        gAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTg             >  2:2159685/1‑76 (MQ=255)

                                                        gAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTg             <  1:5549192/76‑1 (MQ=255)

                                                         aGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGa            >  1:3103021/1‑76 (MQ=255)

                                                          gTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGaa           <  2:915273/76‑1 (MQ=255)

                                                           tGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGaaa          >  1:2646590/1‑76 (MQ=255)

                                                           ggCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGaaa          <  2:5455377/75‑1 (MQ=255)

                                                             cAGCCAGGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGa        <  2:5346969/76‑1 (MQ=255)

                                                              aGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGAt       >  1:4658862/1‑76 (MQ=255)

                                                               cccATGAGCGTATTGGAAGCTGCACCTCTGCCGCTCTCACCACAGCACGCTGACGCACTGCTGAACGTGAAAGAtt      <  1:254160/75‑1 (MQ=255)

                                                                  aTGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGATTTGc   <  2:2060950/76‑1 (MQ=255)

                                                                   tGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGATTTGCg  <  2:1374563/76‑1 (MQ=255)

TTTCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAACGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGATTTGCG  >  NC_000913/1302712‑1302854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`