| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,961,588 | C→G | R86T (AGG→ACG) | gppA ← | guanosine pentaphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,961,588 | 0 | C | G | 99.5% | 494.3 / NA | 184 | R86T (AGG→ACG) | gppA | guanosine pentaphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (100/83); minor base T (0/1); total (100/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 184 total aligned reads displayed.
CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT > NC_000913/3961515‑3961660 | cACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTc > 2:1401081/1‑76 (MQ=255) cccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCg < 1:529416/75‑1 (MQ=255) aCCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCg > 1:1686039/1‑76 (MQ=255) tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCg > 2:1161389/1‑76 (MQ=255) ggaCCGGACAGCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGt < 2:756577/74‑1 (MQ=255) tACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTa < 1:4434648/76‑1 (MQ=255) tACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTa > 2:4812236/1‑76 (MQ=255) gaCCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTa < 2:3684361/75‑1 (MQ=255) cccGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCGCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAg < 2:2278607/75‑1 (MQ=255) aCCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAg < 2:3353332/76‑1 (MQ=255) aCCCGACAACCGAGACTTTCCTGCGCTTTGGCAATAAAACCACCCGCATTGACGGCAAGGCGTAACGTCGCCGCAg > 2:3062828/1‑76 (MQ=255) ccGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGc > 1:3013805/1‑76 (MQ=255) cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCg > 1:4577118/1‑76 (MQ=255) ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGa > 1:4030050/1‑76 (MQ=255) gACAGCCGGGGATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAc < 2:668847/76‑1 (MQ=255) cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa < 2:4299744/76‑1 (MQ=255) cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa > 2:5366144/1‑76 (MQ=255) cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa < 1:4158626/76‑1 (MQ=255) aaCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAc < 2:315644/76‑1 (MQ=255) aaCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAc < 2:1588022/76‑1 (MQ=255) aCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcg < 2:1904303/76‑1 (MQ=255) aCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcg > 1:324977/1‑76 (MQ=255) ccGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcgc > 2:693992/1‑76 (MQ=255) ccGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcgc < 1:1008927/76‑1 (MQ=255) ccGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcgc < 2:796033/76‑1 (MQ=255) cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcgcg > 1:576748/1‑76 (MQ=255) cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcgcg > 1:874914/1‑76 (MQ=255) gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGa > 2:5526728/1‑76 (MQ=255) gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGa > 1:4993970/1‑76 (MQ=255) agaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGaa < 2:1624876/76‑1 (MQ=255) gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAt > 2:1733630/1‑76 (MQ=255) gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAt > 1:2557901/1‑76 (MQ=255) aaaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAt < 1:447192/75‑1 (MQ=255) gaTTTCCTGCGCTTTGGCAATAAAAGCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAtt < 2:5577472/75‑1 (MQ=255) aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAtt < 2:4422733/76‑1 (MQ=255) aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAttt < 1:1034531/76‑1 (MQ=255) aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAttt < 1:4378678/76‑1 (MQ=255) tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTg > 1:2412242/1‑76 (MQ=255) tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTg > 1:1811432/1‑76 (MQ=255) tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTg < 2:1232316/76‑1 (MQ=255) ttCGTGCGCTGTGGCAATAAACTCGCCCGCATTGACGGCAAGGCGTAACGGCGCCGTCGCGCCAGCGCGAATTTGc < 2:2961234/76‑1 (MQ=255) ttCCTGCGCTGTGTCAACAAAATCACCTGCCATGACGCCAAGCACAGACGTCGCCGGAGCGACAACGCGGATTTGc > 2:3469003/1‑76 (MQ=255) cTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgag < 1:5037609/76‑1 (MQ=255) cTGCGCGTTGGCAATAAAAACACCCGCATGGACGGCAAGGCGTAACGGCGCCGTAGCGACAACGCGAATTTGCgag < 2:243935/76‑1 (MQ=255) cgcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgaggga > 1:5554392/1‑76 (MQ=255) gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagggag > 2:3562167/1‑76 (MQ=255) gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagggag > 1:423441/1‑76 (MQ=255) gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagggag < 1:693992/76‑1 (MQ=255) cTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACACCGCGAATTTGCgcgggagg > 2:5770808/1‑76 (MQ=255) cTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagggcgg > 1:5385367/1‑76 (MQ=255) tttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGggagga < 2:4719264/76‑1 (MQ=255) ttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGaa > 2:2688536/1‑76 (MQ=255) ttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGGCGCCGTAGCGACATAGAGCATTTGCGCGGGAGGaa > 1:3866136/1‑76 (MQ=255) ggCAATAAAATCCCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAata < 2:5041597/76‑1 (MQ=255) cAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGACGTAGCGACAACGCGAATTTGTGAGGGAGGGATATc > 1:5505830/1‑76 (MQ=255) aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCt < 2:3243128/76‑1 (MQ=255) aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCt > 1:3725940/1‑76 (MQ=255) aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCtt < 1:2684565/76‑1 (MQ=255) aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCtt < 2:2063351/76‑1 (MQ=255) aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCtt > 2:4946797/1‑76 (MQ=255) aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCtt < 1:1401081/76‑1 (MQ=255) aaaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGc < 2:2948258/76‑1 (MQ=255) aaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCa > 2:5731437/1‑76 (MQ=255) aaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCa < 1:4334442/76‑1 (MQ=255) aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAg > 2:2310640/1‑76 (MQ=255) tcccccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAAGCGAATTTGCGAGGGAGGAATATCTTGCAGAc > 1:354487/4‑76 (MQ=255) tCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGAc < 1:4528216/76‑1 (MQ=255) tCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGAc < 1:4710224/76‑1 (MQ=255) tCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGAc > 2:5226203/1‑76 (MQ=255) cACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACg < 1:3992910/76‑1 (MQ=255) aCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTCGCGAGGGAGCAATATCTTCCACACGt > 2:52831/1‑76 (MQ=255) ccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTc > 1:5154899/1‑76 (MQ=255) cGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCa < 2:2864906/76‑1 (MQ=255) gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAg < 1:52606/76‑1 (MQ=255) aTTGGCGGCCAGGGGTAACTTCGCCGTAGCGACACCGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa < 2:3866135/76‑1 (MQ=255) aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGcg > 2:1828988/1‑75 (MQ=255) aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa > 2:2339581/1‑76 (MQ=255) aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa < 1:3405849/76‑1 (MQ=255) aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa > 1:1862225/1‑76 (MQ=255) aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa > 1:2863354/1‑76 (MQ=255) aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa > 1:3801587/1‑76 (MQ=255) ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCCGACGTTCCGCaa > 2:2606339/1‑76 (MQ=255) ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTAAGCaa > 2:5416648/1‑76 (MQ=255) tGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaac > 2:1248809/1‑75 (MQ=255) gACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc > 2:3504788/1‑76 (MQ=255) aCGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa > 1:376333/1‑76 (MQ=255) aCGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa < 2:4441124/76‑1 (MQ=255) cGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg < 1:4284068/76‑1 (MQ=255) cGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg < 1:5731439/76‑1 (MQ=255) ggCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAgg > 1:2217834/1‑76 (MQ=255) ggCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAgg < 1:5556242/76‑1 (MQ=255) aaGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcgc > 1:197574/1‑76 (MQ=255) aaGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcgc > 1:4653682/1‑76 (MQ=255) aaGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcgc > 2:5796389/1‑76 (MQ=255) aaGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGCTCAGCAAACAGgcgc > 1:1259109/1‑76 (MQ=255) agtcGTCACGTCGCCGCAGCGACACCGCGAATTTGCGAGGGAGGAATATCTTGCAGGCGTTCAGCAAACAGGCGCa < 1:1589299/73‑1 (MQ=255) gCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa < 1:618480/76‑1 (MQ=255) gTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACa > 2:198030/1‑76 (MQ=255) tAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAt < 1:276907/76‑1 (MQ=255) aaCGTCGCCGTAGCGACAACGCGAATTTGCGTGGGAGGAATATCTTGCAGACGTTCAGCAAACCGGCGCAAACAtt < 2:3919252/76‑1 (MQ=255) | CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT > NC_000913/3961515‑3961660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |