| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,978,702 | C→T | S68F (TCC→TTC) | yifK → | putative amino acid/amine transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,978,702 | 0 | C | T | 99.3% | 452.1 / NA | 144 | S68F (TCC→TTC) | yifK | putative amino acid/amine transport protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (81/62); total (81/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 147 total aligned reads displayed.
TCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC > NC_000913/3978629‑3978774 | cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCtg < 2:5041507/76‑2 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc > 1:5493029/1‑76 (MQ=255) aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa < 1:573232/76‑1 (MQ=255) aTTGTGGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa < 2:1175739/76‑1 (MQ=255) ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAac > 2:4951757/1‑76 (MQ=255) tgttgtCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc < 1:1918938/76‑1 (MQ=255) tgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc > 1:262409/1‑76 (MQ=255) gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGCaccg > 2:310550/1‑76 (MQ=255) gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccg > 1:4483754/1‑76 (MQ=255) ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtt > 2:4494682/1‑76 (MQ=255) ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtt < 1:3510138/76‑1 (MQ=255) gtcTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATCGGCGAAATGTTGTTCCTCGAaccggtta < 2:2547953/74‑1 (MQ=255) gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttc > 2:4775524/1‑75 (MQ=255) cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttacc < 2:4143168/76‑1 (MQ=255) tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg < 1:2064976/76‑1 (MQ=255) tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg < 2:2433838/76‑1 (MQ=255) atatCATCGCGGGGCTGTTCGTCTTTTTCATCATGCGTTCAAGGGGCGAAATGTTGTTCCTCGAaccggttaccgg < 2:5390483/76‑1 (MQ=255) tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggt > 1:972166/1‑76 (MQ=255) atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt > 1:962224/1‑76 (MQ=255) tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc > 1:2949977/1‑76 (MQ=255) gtgtcGCCGGGCTGTTCGTCTTTTTCATCGTGCGTTCAATGGGCGACATGTTGTTCCTGGAACCGGGTACCGgttc < 2:1837983/73‑1 (MQ=255) catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcg > 2:5467442/1‑76 (MQ=255) atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt > 1:3676253/1‑76 (MQ=255) atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt < 1:5252828/76‑1 (MQ=255) atcGACGGGCTGTTCGTCTTTTTCATCATGCGTTCAATCACAGAAATGTTGTTACTCGAACAGGTTACCGgttagt > 2:2458897/1‑76 (MQ=255) tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt < 1:5701000/76‑1 (MQ=255) cGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc > 1:3359381/1‑76 (MQ=255) ggcGGGCTTTCCGTCTTTTTCGTCATGCGTTCATTGGGCGAGATGTTGTTCTTCGAACCGGTTTCCGGttcgttcg < 2:2771123/74‑1 (MQ=255) gCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg < 1:4631931/76‑1 (MQ=255) gCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg > 2:4371083/1‑76 (MQ=255) gCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg > 2:389455/1‑76 (MQ=255) gCCGGGCTGTTCGTCTTTTTCATCATGAGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGgttgttcg > 1:78184/1‑76 (MQ=255) cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCTGTTACCGGTTCGTTCGcc > 1:3539871/1‑76 (MQ=255) gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg > 1:4946728/1‑76 (MQ=255) gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg > 1:1793204/1‑76 (MQ=255) gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg < 1:5153512/76‑1 (MQ=255) gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg > 1:4005041/1‑76 (MQ=255) gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg > 1:2562665/1‑76 (MQ=255) gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt < 2:5493027/76‑1 (MQ=255) tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa > 2:1678110/1‑76 (MQ=255) gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt < 1:3612735/76‑1 (MQ=255) gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt > 2:4208207/1‑76 (MQ=255) ttCGTCTTTTTCATCATGCGTACAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATg > 2:3760807/1‑76 (MQ=255) tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc < 2:442340/76‑1 (MQ=255) tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc > 1:4733138/1‑76 (MQ=255) tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc < 1:3952739/76‑1 (MQ=255) gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc > 1:4179430/1‑76 (MQ=255) gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc > 1:5572441/1‑76 (MQ=255) tgtttttGAGCATGCGTTCAATGGGGGAGATGTTGTTCCTCGAACCGGTTACCGGTTTGTTCGCCGTTTATGCGCa < 2:78184/74‑1 (MQ=255) tCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa > 1:4423730/1‑76 (MQ=255) cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccgg > 2:5666760/1‑54 (MQ=255) tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc > 2:3297239/1‑76 (MQ=255) tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc < 2:5664519/76‑1 (MQ=255) ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCg < 2:4958071/76‑1 (MQ=255) ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCg > 2:5005109/1‑76 (MQ=255) ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGtt > 1:4901291/1‑76 (MQ=255) ttCATCATGCGGTCAATGGGCGAAATGTTGGTCCTCGAACCGGTTACCGGATCGTTCGCACATTATGCGCAGCGtt > 2:2716245/1‑76 (MQ=255) tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGttt > 2:1019961/1‑75 (MQ=255) catcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat > 1:2192464/1‑76 (MQ=255) atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata > 2:2195287/1‑76 (MQ=255) tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat > 1:1160990/1‑76 (MQ=255) tGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg > 2:1871001/1‑76 (MQ=255) gCGTTCAATGGTCGACATGTTGTTCCTCGAACCGGTTCCCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc < 2:3639466/76‑1 (MQ=255) gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc > 2:1909688/1‑76 (MQ=255) gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc > 1:1867533/1‑76 (MQ=255) gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCTGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc > 1:4004660/1‑76 (MQ=255) gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc > 1:4249479/1‑76 (MQ=255) gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc > 2:103745/1‑76 (MQ=255) gTTCAATGGGAGAAATGTTGTTCCTCGAACCGGTTACCGGGTCGTTCGCCGTTTATGAGCATCGCTATATGAGccc > 1:4780262/1‑76 (MQ=255) ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg > 2:5090500/1‑76 (MQ=255) ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg < 2:843473/76‑1 (MQ=255) ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg > 2:2535528/1‑76 (MQ=255) ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg < 2:2244778/76‑1 (MQ=255) ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg > 2:1484568/1‑76 (MQ=255) tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt > 2:3412836/1‑76 (MQ=255) tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt > 2:1441522/1‑76 (MQ=255) cAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt < 2:463433/76‑1 (MQ=255) aaTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc > 2:2978813/1‑76 (MQ=255) aaTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc < 1:2923174/76‑1 (MQ=255) aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt < 1:4668965/76‑1 (MQ=255) aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt > 1:1024366/1‑76 (MQ=255) tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt > 2:4628833/1‑76 (MQ=255) tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt > 2:5290071/1‑76 (MQ=255) gggCGAAATGTTGTTCCTCGAACCGGTTACCGgttcg < 1:2179702/37‑1 (MQ=39) gggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt < 2:1949053/76‑1 (MQ=255) gAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt > 1:2079233/1‑76 (MQ=255) gAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt < 1:1032948/76‑1 (MQ=255) aaaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTa > 1:722197/1‑76 (MQ=255) aaaTGCTGTTCCTCGAACAGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTGCTATCGCTa > 2:3422372/1‑76 (MQ=255) aaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAt < 1:322904/76‑1 (MQ=255) aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCtgtt > 1:2642136/1‑73 (MQ=255) aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtc < 2:4370335/76‑1 (MQ=255) aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTCGCTAtc > 2:5075179/1‑76 (MQ=255) gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtctc < 1:1900727/76‑1 (MQ=255) gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtctc > 2:1302961/1‑76 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa > 1:3006558/1‑76 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa > 1:2589580/1‑76 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa < 1:3519933/76‑1 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa < 1:4565558/76‑1 (MQ=255) tgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc < 2:3955650/76‑1 (MQ=255) | TCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC > NC_000913/3978629‑3978774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |