BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,626	T→C	E180E (GAA→GAG)	rspA ←	starvation sensing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,626	0	T	C	100.0%	354.4 / NA	127	E180E (GAA→GAG)	rspA	starvation sensing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (68/59); total (68/59)

Only 100 of 135 total aligned reads displayed.

ACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGT  >  NC_000913/1652554‑1652697

aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                      >  1:2098570/1‑76 (MQ=255)

 ccAAACTTGTTACGTACCGCGT‑AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                    >  2:3213098/1‑76 (MQ=255)

   aaaCTTGTTGCGTACCGCGTCAATCATTTTCGGGATTAAATCTAGGTATTTCTCCTTGGACCGCAGCTGCTCTTcc                                                                   <  2:1909524/76‑1 (MQ=255)

    aaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                  <  1:1048551/76‑1 (MQ=255)

     aCTTGTTACGTACCGCGTCAAACAATTTCGGCATAAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCgg                                                                 >  2:2855776/1‑76 (MQ=255)

      ctggTTACGTGCCGCGTCAAACAATTTCGGCATGAAAGCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                <  1:5397057/73‑1 (MQ=255)

      cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                <  2:1493854/76‑1 (MQ=255)

      cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                >  2:3852521/1‑76 (MQ=255)

      cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                >  1:4924425/1‑76 (MQ=255)

       ttGTTACGTGCCTCGTCAAACAATTGCTGCCTGAGCTCGAGGTATTTGTCCGTCTCCCTCCGCTGCTCTTCCGGcc                                                               <  2:3726826/76‑1 (MQ=255)

       ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGcc                                                               >  2:1188923/1‑76 (MQ=255)

         gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                             <  1:4354948/76‑1 (MQ=255)

         gTTACGGACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                             <  2:4889486/76‑1 (MQ=255)

            aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTgc                                                          >  2:1382994/1‑75 (MQ=255)

            aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                          <  2:5336997/76‑1 (MQ=255)

             cGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTc                                                         >  1:4344170/1‑76 (MQ=255)

              gtcccGCGTCAAACAATTGCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                        <  2:1914212/73‑1 (MQ=255)

              gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                        >  1:3789/1‑76 (MQ=255)

                cccGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                      <  2:557977/75‑1 (MQ=255)

                 ccGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                     >  2:3497160/1‑76 (MQ=255)

                 ccGCGTCAAACAAGTGCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGGCCttt                                                     <  1:4385414/76‑1 (MQ=255)

                  cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCCCCGTCCTTTg                                                    >  2:743247/1‑76 (MQ=255)

                  cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                    >  1:3359922/1‑76 (MQ=255)

                  cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                    <  1:5557180/76‑1 (MQ=255)

                   gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                   >  1:4480818/1‑76 (MQ=255)

                   cagTCAAACAATGGCGGCATGCAATCGAGGTCTTTATCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                   <  2:2845787/74‑1 (MQ=255)

                    cgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGt                                                  >  2:4682667/1‑76 (MQ=255)

                     gTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGtt                                                 <  2:3050048/76‑1 (MQ=255)

                      tCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTCTCCTGTGGTTg                                                >  1:3290121/1‑76 (MQ=255)

                        aaaaaaTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                              >  1:3087298/1‑76 (MQ=255)

                         aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                             >  2:4421074/1‑76 (MQ=255)

                          aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                            >  2:1340666/1‑76 (MQ=255)

                             aTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTc                                         >  2:3536481/1‑76 (MQ=255)

                              tttCGGCATGAAATCGAGGTATTTCTCCGTCGTCCACAGCTGCTCTTCCGGCCTCTGTCCTTTGGTTGCTTGTTCa                                        <  1:743247/76‑1 (MQ=255)

                               ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                       >  1:456078/1‑76 (MQ=255)

                               tgcGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                       <  2:5587475/74‑1 (MQ=255)

                                tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                      >  2:2253130/1‑76 (MQ=255)

                                 cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                     <  1:3233233/76‑1 (MQ=255)

                                 cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                     <  1:986586/76‑1 (MQ=255)

                                 cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                     >  2:4692915/1‑76 (MQ=255)

                                 cGGCAGGAAAGCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                     <  1:4862144/76‑1 (MQ=255)

                                   gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGc                                   >  2:1911139/1‑76 (MQ=255)

                                   gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACCGTCCTTTGGTTGCGGGTTCATAAGc                                   >  2:5464899/1‑76 (MQ=255)

                                     aTGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATACGCCa                                 >  1:3691757/1‑76 (MQ=255)

                                     aTGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGccc                                 >  2:1630373/1‑75 (MQ=255)

                                      tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAg                                <  2:5543952/76‑1 (MQ=255)

                                      tGAAATCGAGGTATTCCTCCGCCGACCACAGCTGCTCCTCCGGCCACTCCCCTTTGGTTCCGGGCTCATCAGACac                                >  2:4726247/1‑75 (MQ=255)

                                        aaaTCGGGGTATGTCTCCGTCGACCACGGCTGCTCTTCAGGCTACTGTCCTTTGGTTGCGGGCTCATACGCCAGac                              >  2:1469049/1‑76 (MQ=255)

                                        aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGac                              >  2:5035354/1‑76 (MQ=255)

                                        aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGac                              >  1:1033191/1‑76 (MQ=255)

                                         aagcGAGGTATTGCTCCGTCGGCCACATCGTCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                             <  2:2088583/73‑1 (MQ=255)

                                         aaTCGAGGTATTTCTCGGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                             <  1:305041/76‑1 (MQ=255)

                                          aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                            >  1:4136371/1‑76 (MQ=255)

                                           tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCGACTGTCCTTTGGTTGCGGGTTCATAAGCCAGccctt                           <  2:4831358/76‑1 (MQ=255)

                                            cGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                          >  1:4105514/1‑76 (MQ=255)

                                              aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                        <  1:3313764/76‑1 (MQ=255)

                                              aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                        <  1:1968578/76‑1 (MQ=255)

                                                  ttttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttaccttt                    <  2:5477667/75‑1 (MQ=255)

                                                  aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttaccttt                    <  1:5412097/76‑1 (MQ=255)

                                                   tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   >  1:3058809/1‑76 (MQ=255)

                                                   tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   <  2:3669813/76‑1 (MQ=255)

                                                   tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   >  2:1075307/1‑76 (MQ=255)

                                                   tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   <  2:3283196/76‑1 (MQ=255)

                                                   tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACATTTc                   >  2:4953002/1‑76 (MQ=255)

                                                    ttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                  >  2:4888001/1‑76 (MQ=255)

                                                     tctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGa                 >  1:1038630/1‑76 (MQ=255)

                                                     tctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGa                 <  2:4105513/76‑1 (MQ=255)

                                                     tctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGa                 >  1:273533/1‑76 (MQ=255)

                                                      ctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAc                >  2:4280430/1‑76 (MQ=255)

                                                      cgacGTCAACCACCTCTGCTCTACCTGCCACTGTCCTTTGGTTGCGTGGTCATAATCCAGACCTTTCCCTTACGAc                <  1:3660655/73‑1 (MQ=255)

                                                       tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa               <  2:4583690/76‑1 (MQ=255)

                                                        ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt              <  1:3560681/76‑1 (MQ=255)

                                                        ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt              >  1:1296193/1‑76 (MQ=255)

                                                         ngTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg             >  1:5391074/2‑76 (MQ=255)

                                                          gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGCCCTTTACCTTTCGACATGc            >  2:501322/1‑76 (MQ=255)

                                                          gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            >  2:5130556/1‑76 (MQ=255)

                                                          gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            <  2:899157/76‑1 (MQ=255)

                                                          gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            >  1:2616977/1‑76 (MQ=255)

                                                          gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            <  1:769798/76‑1 (MQ=255)

                                                          gTCGACCACAGCTGCTCTTCCGGCCACTGTCCCTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTGCGACATGc            >  1:1740232/1‑76 (MQ=255)

                                                           tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc           <  2:2216276/76‑1 (MQ=255)

                                                           tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc           >  2:1381586/1‑76 (MQ=255)

                                                           gccaCCACAGCTGCTCTGCCGGCCACTGGCCGTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc           <  1:3252908/73‑1 (MQ=255)

                                                            cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACACGCCg          >  2:2126641/1‑76 (MQ=255)

                                                            agACCACAGCTGCTCGTCCGGGCACTGACCTTTGGTTGCAGCGTAATAACACAGACCTTTACCTGACGACATGCCg          >  1:1959452/2‑76 (MQ=255)

                                                             gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         <  1:4087313/76‑1 (MQ=255)

                                                             gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         <  1:5580209/76‑1 (MQ=255)

                                                             gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         >  2:3582325/1‑76 (MQ=255)

                                                             gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         >  2:5112142/1‑76 (MQ=255)

                                                              aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa        >  1:1504717/1‑76 (MQ=255)

                                                               ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg       >  2:3225331/1‑76 (MQ=255)

                                                               ccACAGCTGCGCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg       <  2:5779250/76‑1 (MQ=255)

                                                                cgcaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATACGCCAGACCTTTACCTTTCTACATGCCGTAgg      <  1:2668991/74‑1 (MQ=255)

                                                                cacaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAgg      <  1:3817176/76‑1 (MQ=255)

                                                                cacaGCTGCTCTCCCGTCCCCTGTCCTTTGGTTTCGTGTTCATAAGCCACACCTTTACCTTTCCACATGCCGTAgg      <  1:4687673/76‑1 (MQ=255)

                                                                 acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt     <  1:3775708/76‑1 (MQ=255)

                                                                 acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt     <  1:784666/76‑1 (MQ=255)

                                                                    gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt  >  1:4887376/1‑76 (MQ=255)

                                                                    gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt  >  2:2573917/1‑76 (MQ=255)

                                                                    gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt  <  2:347340/76‑1 (MQ=255)

ACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGT  >  NC_000913/1652554‑1652697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`