BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,743	C→T	P101L (CCC→CTC)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,743	0	C	T	98.6%	435.1 / NA	143	P101L (CCC→CTC)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (67/74); minor base G (1/1); total (68/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT  >  NC_000913/4016671‑4016813

    aGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATg                                                                 <  2:2505173/76‑1 (MQ=255)

      cGAAATTCTCCCGCCTGGTCGGAGACACTGGCAAAGTGGTCCTTGCTGATCTCAATGAATACATGCTCACAATgtg                                                               >  2:1062349/1‑74 (MQ=255)

      cGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATggg                                                               >  2:1049662/1‑76 (MQ=255)

       gAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                              <  2:2379880/76‑1 (MQ=255)

        aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                             <  2:5735225/76‑1 (MQ=255)

         aaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcg                                                            <  1:228549/76‑1 (MQ=255)

          aTTCTCCCGCCTGGTGGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgc                                                           <  2:5379941/76‑1 (MQ=255)

           ttCTCCCGCCTGGTCGGAGAAACTGGTAAAGTGGCCCGTGATGAGATCAATGAATCTAGGCGCATAATGAGAcgcg                                                          >  1:4615757/1‑76 (MQ=255)

           ttCTCCCGCCTGGTCGGAGAAACTGGCAACGGGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgcg                                                          <  2:608653/76‑1 (MQ=255)

           ttCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgcg                                                          <  2:2240555/76‑1 (MQ=255)

           ttCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgcg                                                          <  1:524592/76‑1 (MQ=255)

            tctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCga                                                         >  1:4561922/1‑76 (MQ=255)

             ctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                        <  2:1617928/76‑1 (MQ=255)

             ctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                        <  2:1223813/76‑1 (MQ=255)

             ctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGAACTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                        >  1:113689/1‑76 (MQ=255)

              tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                       >  1:5202733/1‑76 (MQ=255)

              tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                       <  1:4448238/76‑1 (MQ=255)

              tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                       >  2:4186877/1‑76 (MQ=255)

              tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                       >  2:3278491/1‑76 (MQ=255)

              tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                       >  2:353462/1‑76 (MQ=255)

              tcCCGCCGGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                       <  2:4718238/76‑1 (MQ=255)

                ccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAg                                                     >  1:5447375/1‑76 (MQ=255)

                 cGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                    >  1:2663187/1‑76 (MQ=255)

                 cGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                    <  2:1948770/76‑1 (MQ=255)

                   ccTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTg                                                  <  2:1446878/76‑1 (MQ=255)

                   ccTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTg                                                  >  1:4462287/1‑76 (MQ=255)

                   ccTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATACATGCTCAAAATGGGCCGCGAGAAGCTg                                                  >  2:3556062/1‑76 (MQ=255)

                    cTGGTCGGAGACACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCtcc                                                 >  2:5822649/1‑74 (MQ=255)

                    cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGc                                                 <  2:3032390/76‑1 (MQ=255)

                    cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGc                                                 <  1:194752/76‑1 (MQ=255)

                     tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                >  2:3318161/1‑76 (MQ=255)

                     tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                >  2:5053976/1‑76 (MQ=255)

                      ggTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGt                                               >  2:1353271/1‑76 (MQ=255)

                        tCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                             <  2:2971520/76‑1 (MQ=255)

                        tCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                             >  2:4569106/1‑76 (MQ=255)

                        tCGGAGAAACTAGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                             <  1:157234/76‑1 (MQ=255)

                         cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAat                                            <  1:2218871/76‑1 (MQ=255)

                          ggAGAACCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCCAAATGGGCCGCGAGCAGCTGCGTAata                                           >  2:2900073/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                           >  2:5674969/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                           >  2:2876514/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                           >  2:789615/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                           >  2:1211120/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                           >  1:2444469/1‑76 (MQ=255)

                           gagaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                          >  1:4783651/1‑76 (MQ=255)

                           gagaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                          <  2:4012115/76‑1 (MQ=255)

                           gagaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                          <  2:2444469/76‑1 (MQ=255)

                              aaaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                       <  1:114011/76‑1 (MQ=255)

                              aaaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                       <  1:4569107/76‑1 (MQ=255)

                              aaaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                       <  1:117901/76‑1 (MQ=255)

                              aaaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATACATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                       >  2:5037092/1‑76 (MQ=255)

                               aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                      <  1:3894717/76‑1 (MQ=255)

                               aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                      >  2:4069342/1‑76 (MQ=255)

                                aCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                     >  2:5121975/1‑76 (MQ=255)

                                aCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                     <  1:3306150/76‑1 (MQ=255)

                                 cTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtgt                                    >  1:4058335/1‑76 (MQ=255)

                                  tGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                   >  1:43205/1‑76 (MQ=255)

                                  tGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                   <  1:1049662/76‑1 (MQ=255)

                                  tGGCAAAGTGGTCATTGCGGATATCAATGAAGCAAGTCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                   <  1:1062349/76‑1 (MQ=255)

                                   ggCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATAGGTGTGa                                  <  1:1101500/76‑1 (MQ=255)

                                   cgCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGa                                  >  1:3468880/2‑76 (MQ=255)

                                     cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                <  1:789615/76‑1 (MQ=255)

                                     cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                >  2:4194021/1‑76 (MQ=255)

                                      aaaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                               <  2:5640060/76‑1 (MQ=255)

                                       aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTgg                              >  1:5580793/1‑76 (MQ=255)

                                       aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTgg                              >  1:2097293/1‑76 (MQ=255)

                                        aggggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                             <  2:5810810/73‑1 (MQ=255)

                                         gTGGTCCTGGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCa                            >  2:2003071/1‑76 (MQ=255)

                                          gggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGTCaa                           <  2:5157315/75‑1 (MQ=255)

                                           ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                          <  2:4608904/76‑1 (MQ=255)

                                           ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                          <  1:2910078/76‑1 (MQ=255)

                                           ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                          >  1:66618/1‑76 (MQ=255)

                                             tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                        <  2:872904/76‑1 (MQ=255)

                                             tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                        >  1:1646724/1‑76 (MQ=255)

                                              ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                       >  2:3586660/1‑76 (MQ=255)

                                              ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAACTGCGTAATATCGGTGTGATTGGCAACGtt                       >  1:933909/1‑76 (MQ=255)

                                                ttGCTGATATCAATGAATCAATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTgc                     >  2:3831952/1‑75 (MQ=255)

                                                gtGCTGATGTAACTGAATCCGTGCTCTAAGTGGGCTGTGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                     <  2:3210257/75‑1 (MQ=255)

                                                 tGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAg                    >  2:3709278/1‑76 (MQ=255)

                                                 tGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAg                    >  2:4833286/1‑76 (MQ=255)

                                                  gCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGt                   >  1:206291/1‑76 (MQ=255)

                                                  gCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGt                   >  2:1676724/1‑76 (MQ=255)

                                                   cTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTa                  >  1:822261/1‑76 (MQ=255)

                                                    tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAt                 <  2:2850112/76‑1 (MQ=255)

                                                    tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAt                 >  1:4216049/1‑76 (MQ=255)

                                                    tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAt                 <  1:5004067/76‑1 (MQ=255)

                                                    tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAt                 <  1:5824871/76‑1 (MQ=255)

                                                        atCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTc             <  2:341015/76‑1 (MQ=255)

                                                        atCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTc             <  2:2088273/76‑1 (MQ=255)

                                                         tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa            >  2:5155773/1‑76 (MQ=255)

                                                         tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa            <  2:3125423/76‑1 (MQ=255)

                                                         tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa            >  1:2355332/1‑76 (MQ=255)

                                                         tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa            >  1:1628481/1‑76 (MQ=255)

                                                           aaTGAATCCGTGCTGAAAATGGGCTGCGAGAAGCTGCGTAATATCGGTGTGATTGGCTACGTTGAGTATGTTCAgg          <  2:4972466/76‑1 (MQ=255)

                                                           aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAgg          <  2:4216018/76‑1 (MQ=255)

                                                            aTGAATCCATGCGCAAAATGGGCCGCGAGAAGCTGCGTAATATCTGTGTGATTGGCAACGTTGAGTATGTTCAGGc         <  2:654330/76‑1 (MQ=255)

                                                             tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCg        >  1:1286696/1‑76 (MQ=255)

                                                               aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa      <  1:5488804/76‑1 (MQ=255)

                                                                 tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg    <  2:1646724/76‑1 (MQ=255)

                                                                 tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg    <  1:4056821/76‑1 (MQ=255)

                                                                   cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCt  >  2:3470786/1‑76 (MQ=255)

TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT  >  NC_000913/4016671‑4016813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`