| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,019,857 | C→T | intergenic (+56/‑28) | b3836 → / → b3838 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,019,857 | 0 | C | T | 98.3% | 158.1 / NA | 59 | intergenic (+56/‑28) | b3836/b3838 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (25/33); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCGC > NC_000913/4019787‑4019919 | aaaGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCCTCATCGGCCtcgtcgttt > 2:312295/1‑73 (MQ=255) cAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc > 1:2132888/1‑76 (MQ=255) cAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc > 2:5363908/1‑76 (MQ=255) cAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc < 2:5174520/76‑1 (MQ=255) cAGGTGTAATCCGTGTTTGATATCGGGTTTAGCGCACTGCTATTGGTGTTCATCCTCGGCCTCGTCG‑‑TTCTGGGGc > 2:5274854/1‑76 (MQ=255) cAGGTGTAAGCCGGGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc < 2:2161970/76‑1 (MQ=255) aGGTGTAGTCCGTGTTGGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc < 2:2130199/76‑1 (MQ=255) aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc < 2:3792348/76‑1 (MQ=255) aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc > 2:3671578/1‑76 (MQ=255) ggTGTAACCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCg < 2:3182830/76‑1 (MQ=255) gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc > 1:1435068/1‑76 (MQ=255) gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc > 2:2267447/1‑76 (MQ=255) gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc > 2:4823168/1‑76 (MQ=255) gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCaa < 1:4853023/76‑1 (MQ=255) gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCaa > 1:3372847/1‑76 (MQ=255) tAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAc < 1:3838523/76‑1 (MQ=255) tAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAc < 1:298040/76‑1 (MQ=255) tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc < 2:4858978/76‑1 (MQ=255) tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc > 2:636122/1‑76 (MQ=255) tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCAACATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc < 1:2555780/76‑1 (MQ=255) ccGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACt > 2:3608668/1‑76 (MQ=255) ccGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACt > 1:1715880/1‑76 (MQ=255) ccGTGTTTGATATCGGTTTTAGCGAACTGCTATTAGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACt < 1:18821/76‑1 (MQ=255) cGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTg > 2:2421858/1‑76 (MQ=255) tgtTTGATATCGGTGTTAGAGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGCGCCGCAACGACTGcc > 1:1464259/1‑76 (MQ=255) gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCt < 1:4537235/76‑1 (MQ=255) gATATCGGTTTTAGCGATCTGTGATGGGGGTTCATCCCCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg < 2:4482844/76‑1 (MQ=255) gATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg > 2:3130510/1‑76 (MQ=255) gATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg > 1:2852725/1‑76 (MQ=255) gATATCGGTTTGAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg < 2:2839809/76‑1 (MQ=255) tttCGGTTTTAGCGGACTGCTATGGGTGTTCATCATCGGCTTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑g < 2:600621/74‑1 (MQ=255) tatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑g > 2:218415/1‑76 (MQ=255) atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg > 1:4959346/1‑76 (MQ=255) atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg > 1:4944210/1‑76 (MQ=255) atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg > 1:318160/1‑76 (MQ=255) ggTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gggga > 2:3983744/1‑73 (MQ=255) ggTTTTAGCGAACTGCTATTGGTGTTCAGCATGGGCCTCGTCG‑‑TTCGGGGGCCGCAACGACTGCCTGTGGC‑GGTaa < 2:4207792/76‑1 (MQ=255) ttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAAc < 1:1189127/76‑1 (MQ=255) tAGCGACCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACg < 2:1382968/76‑1 (MQ=255) tAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACg < 2:4551315/76‑1 (MQ=255) aGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGGAAAAACg > 2:3400098/1‑76 (MQ=255) cGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTa < 1:984761/76‑1 (MQ=255) gaccTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAg < 1:1234255/73‑1 (MQ=255) gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGGAAAACGGTAg > 1:251592/1‑76 (MQ=255) gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGGAAAAAGGGAg > 1:5633330/1‑76 (MQ=255) aaCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGc < 2:4944209/76‑1 (MQ=255) aaCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGc < 2:1102278/76‑1 (MQ=255) aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGGAAAACAGTAACg > 2:818831/1‑76 (MQ=255) aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGAGAAAACGGGAGCg > 1:2572996/1‑76 (MQ=255) cTATTGTGGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCt < 1:433331/76‑1 (MQ=255) cTATTGGTTTTCATCACCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCt < 2:4190530/76‑1 (MQ=255) tgttGGTGTTCAGCATCGGCCTCGTCG‑‑TGCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTg < 2:5066464/74‑1 (MQ=255) tATTGGTGTTCATCATGGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTg < 2:1911750/76‑1 (MQ=255) tATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTg < 1:3130510/76‑1 (MQ=255) tGGTGTTCATCAGCGGCCTCGTCG‑‑TTCGGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGAt < 2:5401464/76‑1 (MQ=255) tgtTCATCATCGGCCTCGTCGGC‑‑CTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTcg < 2:1151220/76‑1 (MQ=255) tttCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTcgc < 1:768610/75‑1 (MQ=255) gtTCTTCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTcgc < 2:4570495/76‑1 (MQ=255) tcatcaTCGGCCTTGCCG‑‑TTCTGCGGCCGCAACGACTGCCTGTGGC‑GGGAAAAAAGGGCGCAGGGTGGTGTcgcgg > 1:5718328/1‑75 (MQ=255) tcatcaTCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTcgcgc < 1:2152868/76‑1 (MQ=255) | AAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCGC > NC_000913/4019787‑4019919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |