| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,718,191 | A→G | T98A (ACT→GCT) | slyB → | putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,718,191 | 0 | A | G | 99.3% | 380.8 / ‑5.8 | 142 | T98A (ACT→GCT) | slyB | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (56/83); minor base T (0/1); total (58/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
Only 100 of 142 total aligned reads displayed.
CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGT > NC_000913/1718118‑1718262 | catGGGGAATACTGTTGGGGGCGGAACCGGGCGATCGATAGCGACTAAAGCAGGCGCTGTTGCTGGCGGCGTAACt > 1:585839/3‑76 (MQ=255) tGGGGAGTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg < 2:4647265/76‑1 (MQ=255) tGGGGAATACTGTTGGTGGCTGATCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGTCGTAGCTgg < 1:4461376/76‑1 (MQ=255) gggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc < 2:1412555/76‑1 (MQ=255) gggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc < 2:5445443/76‑1 (MQ=255) gtaaTACGCTTGGGGGGGGAACCTGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCa < 2:471584/74‑1 (MQ=255) ggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGGGGCGGAGCTGGtgc > 2:552352/1‑74 (MQ=255) ggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAAGTGGCGTAGCTGGTCa < 1:458710/76‑1 (MQ=255) aaTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAgg < 1:3590671/76‑1 (MQ=255) aTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAggg > 1:4315098/1‑76 (MQ=255) tgcTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTGCTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc < 2:5612057/74‑1 (MQ=255) tACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc < 1:956896/76‑1 (MQ=255) tACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGGGGCGGAGCTGGTCAgggg > 1:5262744/1‑75 (MQ=255) gcTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCg < 2:5686186/75‑1 (MQ=255) aCTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCg < 2:4015739/76‑1 (MQ=255) cTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGt < 2:2850028/76‑1 (MQ=255) cTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGGTGCAGGTGGCGTAGCTGGGCAGGGCgg > 1:3535839/1‑75 (MQ=255) gTTGGTGGCGGATCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAc < 1:2795067/76‑1 (MQ=255) ttGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCCGGTGGCGTAGCTGGTCAGGGCGTACa > 2:1169264/1‑76 (MQ=255) ttGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACa < 1:5838976/76‑1 (MQ=255) ttGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGGAGCTGGTCAGGGCGTACa > 2:4243155/1‑76 (MQ=255) tggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACag < 2:1192165/76‑1 (MQ=255) ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACagg > 1:486149/1‑75 (MQ=255) ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga < 1:4607585/76‑1 (MQ=255) ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga < 1:5375876/76‑1 (MQ=255) ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga < 2:1204742/76‑1 (MQ=255) gtggCGGACCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACagag < 1:1173181/76‑1 (MQ=255) tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTagagagg > 1:1222980/1‑75 (MQ=255) tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGt < 2:5311571/76‑1 (MQ=255) ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGCGGCGTAGCTGGTCAGGGCGTACAGAGTg < 1:3471290/76‑1 (MQ=255) ggCGGAACCGGGCGTTCTCTGGCGACTGCAGCAGGCTCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTg < 1:5446895/76‑1 (MQ=255) gCGGAGCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGc < 1:2503500/76‑1 (MQ=255) ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCaa < 1:2881257/76‑1 (MQ=255) ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCaa < 1:1398080/76‑1 (MQ=255) ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGGAGCTGGGCAGGGGGTCCCGAGGGCaa > 2:5243814/1‑76 (MQ=255) ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGGGGCGTAGCTGGTCAGGGCGGACAGAGTGCaa > 2:3728146/1‑76 (MQ=255) ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCCGTCGCAGGCGGCGCACCTCGTCCGGGGGGACAGCATGCaa > 1:4147995/1‑76 (MQ=255) aaCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATg > 1:1735528/1‑76 (MQ=255) aaCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATg < 1:1360187/76‑1 (MQ=255) aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGa < 1:5077399/76‑1 (MQ=255) ccGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGaa < 1:4966281/76‑1 (MQ=255) ccGGGCGTTCCCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGaa > 1:283404/1‑76 (MQ=255) cGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCAATGaaa > 2:4249294/1‑75 (MQ=255) gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACa < 1:3407993/76‑1 (MQ=255) gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACa > 1:5412442/1‑76 (MQ=255) gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACa > 2:2515010/1‑76 (MQ=255) gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGCGGCGTAGCTGGTCAGGGCGGACAGAGGGCAATGcaca > 2:491984/1‑76 (MQ=255) ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaa < 1:1530584/76‑1 (MQ=255) gacGTTCTCTGCCTACTGCAGCAGGCTCTTCTTCATGTGGCGTCTCTGCTCAGTGCCTTCTGTCTGCAATGAACac < 1:2295063/74‑2 (MQ=255) gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCAATGAACaaa > 1:5518640/1‑76 (MQ=255) cGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaaa < 2:1021523/76‑1 (MQ=255) cGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaaa < 1:5659237/76‑1 (MQ=255) cGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaaa > 2:4542382/1‑76 (MQ=255) cGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaaa < 2:3445886/76‑1 (MQ=255) cGTTCTCTGGCTACTGCAGCAGGAGCTGTTGCAGCTCGCGTAGCTGGTCAGGGCGGAAAGAGTGCAATGAACaaaa > 2:2508616/1‑76 (MQ=255) gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAc > 1:3838311/1‑76 (MQ=255) tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGc < 1:544376/76‑1 (MQ=255) tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGc > 2:2663839/1‑76 (MQ=255) tctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg > 1:2772931/1‑76 (MQ=255) tctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg > 2:3013387/1‑76 (MQ=255) tctGGCTACTGCAGCAGGCGCTGGTGCAGGTGGCGTAGCTGGTCAGGGCGGACAGAGTGCAATGAACAAAACGGAg > 1:4969855/1‑76 (MQ=255) ggCTACTGCAGCGGGCGCTGTTGCCGGTGGCGAAGCTGGTCAGGGCCTACCGAGCGCAATGAACAAAACCCAgggg > 2:2309632/1‑75 (MQ=255) gCTACTGCCGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg < 2:3002772/76‑1 (MQ=255) gCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg < 1:3012893/76‑1 (MQ=255) cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtgt < 1:3959750/76‑1 (MQ=255) tACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTc < 2:4209956/76‑1 (MQ=255) cTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGa < 1:2231016/76‑1 (MQ=255) gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGc < 2:2742069/76‑1 (MQ=255) cagcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt > 2:837422/1‑76 (MQ=255) cagcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt < 2:3433179/76‑1 (MQ=255) agcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACACAGGGGGTCGAGCTg > 2:2106078/1‑76 (MQ=255) gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTgg < 2:5200475/76‑1 (MQ=255) cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa < 2:5373284/76‑1 (MQ=255) agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaa > 2:3899902/1‑76 (MQ=255) agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaa < 1:3815573/76‑1 (MQ=255) ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa < 1:332168/76‑1 (MQ=255) ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa < 2:2798754/76‑1 (MQ=255) gcgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAt < 1:1754177/76‑1 (MQ=255) ggcTGGTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAtt < 2:5520429/75‑1 (MQ=255) cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAtt > 2:5175870/1‑76 (MQ=255) cgcTGGTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAtt < 2:445859/76‑1 (MQ=255) tcTGTTGGAGGTGGAGTAGGTGGTCAGTGCGTACAGAGTGCAATAACCAAAACGCAGGGTGTCGAGCTGGAACTTc < 1:5641909/75‑1 (MQ=255) gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTc > 2:5225588/1‑76 (MQ=255) gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTc < 1:1192870/76‑1 (MQ=255) gcTGTTGCAGGTGGCGTAGCTGGTCAGCGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTc < 2:910172/76‑1 (MQ=255) cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAACCGCAGGGTTTCGAGCTGGAAATTCg < 2:2819336/76‑1 (MQ=255) tGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGt > 2:2256885/1‑76 (MQ=255) gTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa > 1:5403720/1‑76 (MQ=255) gTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa > 1:3949561/1‑76 (MQ=255) ttGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaa < 1:5137791/76‑1 (MQ=255) tGCAGGTGGCGTAGCTGGTCAGGGCGTACAGGGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaaa > 2:5126912/1‑76 (MQ=255) tcAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAg < 2:5077540/75‑1 (MQ=255) gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAg < 2:3465742/76‑1 (MQ=255) aGGTGGCGTAGCTGGGCAGGGCGTAAAGAGTGCAATGAAAAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc > 2:5623785/1‑76 (MQ=255) ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACg > 2:5576471/1‑76 (MQ=255) ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACg > 2:5640594/1‑76 (MQ=255) gTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGa < 2:3962200/76‑1 (MQ=255) tGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt < 1:5243815/76‑1 (MQ=255) cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt < 2:1836135/76‑1 (MQ=255) cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt > 1:2064276/1‑76 (MQ=255) | CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGT > NC_000913/1718118‑1718262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |