| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,091,351 | C→T | G168D (GGC→GAC) | rhaD ← | rhamnulose‑phosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,091,351 | 0 | C | T | 98.0% | 424.2 / ‑3.3 | 151 | G168D (GGC→GAC) | rhaD | rhamnulose‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (70/77); total (73/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01 | |||||||||||
Only 100 of 158 total aligned reads displayed.
TCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG > NC_000913/4091279‑4091423 | tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCtt < 2:2223650/76‑1 (MQ=255) cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc > 2:4111916/1‑76 (MQ=255) cccgggccgggCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc < 2:625849/75‑1 (MQ=255) ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCca < 2:1561890/76‑1 (MQ=255) ggTGCGGGGCCCCAGCCGCGGCAAAATGGCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac < 2:408853/75‑1 (MQ=255) cGTGCCGGGCACCATCCACGGCAAAATGCTAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac > 1:4983848/1‑76 (MQ=255) tGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAg > 1:2294815/1‑76 (MQ=255) gcggggCAGCATCCGCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt < 2:2406412/73‑1 (MQ=255) gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCCGACACTCGGTGCCGTCTTCCCACAGt > 2:5464616/1‑76 (MQ=255) gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt > 1:5410241/1‑76 (MQ=255) gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt > 1:4777087/1‑76 (MQ=255) ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt < 1:5253162/76‑1 (MQ=255) ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt < 1:2349918/76‑1 (MQ=255) cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg < 1:4049887/76‑1 (MQ=255) ggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCAACAGTTGGc < 2:2423627/76‑1 (MQ=255) gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACCGTTGGCg > 2:3781493/1‑76 (MQ=255) gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACCGTTGGCg > 1:1436518/1‑76 (MQ=255) gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGCTGGCg > 2:165484/1‑76 (MQ=255) cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa < 2:3859130/76‑1 (MQ=255) aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg < 1:53895/76‑1 (MQ=255) aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGCTGGCggg > 2:597144/1‑74 (MQ=255) ccacccacGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt < 2:5092443/76‑1 (MQ=255) ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt < 2:4156771/76‑1 (MQ=255) ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt < 1:1203383/76‑1 (MQ=255) aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa > 2:3518040/1‑76 (MQ=255) aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa > 1:3494457/1‑76 (MQ=255) tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa < 2:3094511/76‑1 (MQ=255) tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa > 1:3986468/1‑76 (MQ=255) ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg < 2:3592919/76‑1 (MQ=255) ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg < 2:951543/76‑1 (MQ=255) cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa < 1:3924049/76‑1 (MQ=255) cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa > 2:4468601/1‑76 (MQ=255) ccGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc < 1:2222843/75‑1 (MQ=255) cGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc < 2:2091298/76‑1 (MQ=255) cGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc < 1:299364/76‑1 (MQ=255) ggCAAAATGCCAACGCCGTCCGGGAATACCACCAGACACTCTGTGCTGTCTTCCCCCAGTTGGCGAGTGAAGACcg < 2:391477/76‑1 (MQ=255) ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcg < 2:584495/76‑1 (MQ=255) ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcg > 1:1205895/1‑76 (MQ=255) gCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc < 2:1233639/76‑1 (MQ=255) gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGTTGCTGTCTTCCCACCGTTGGCGAGCGAAGACcgc > 1:1010668/1‑76 (MQ=255) gtgaaTGCCAACGCCAGCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg < 1:5786410/73‑1 (MQ=255) cAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg > 2:5630846/1‑76 (MQ=255) aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt < 2:3634785/76‑1 (MQ=255) aaaTGCAAGCGCCATCCGGGAAGACCACCAGACACTCGGTGCTGGCTTCCCACAGTTGGCGAGTGAAGACCGCGgt < 2:3656865/76‑1 (MQ=255) aaTGCCAACGCGATCGGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg < 2:5733041/76‑1 (MQ=255) aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg < 1:943798/76‑1 (MQ=255) aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg < 2:2294815/76‑1 (MQ=255) aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg > 2:4134018/1‑76 (MQ=255) aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt > 2:725957/1‑76 (MQ=255) tGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTc > 1:3002164/1‑76 (MQ=255) tGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTc > 2:4812949/1‑76 (MQ=255) gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg < 1:3422758/76‑1 (MQ=255) aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt > 2:1058321/1‑76 (MQ=255) aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTTGCGAGAGAAGACCGCGGCGTCGtttt > 2:309329/1‑76 (MQ=255) aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt > 1:2576597/1‑76 (MQ=255) aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttct > 1:3189988/1‑74 (MQ=255) cGCCATCCGGTAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc < 2:3942934/76‑1 (MQ=255) cATCCGGGAATACCACCAGACGCTGGGTGCGGTCTGCCCACAGTTGGCGAGTGAAGGCCGCGGTGTCGTTTTCAAg < 2:184904/76‑1 (MQ=255) tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAgca > 1:631003/1‑74 (MQ=255) ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGGGGCGTTTTCAAGTAc > 2:2885575/1‑76 (MQ=255) ccGGGAATACCACCAGACACTCGGTGCTGCATTCCCACCGTTGGCGCGTCACGCCCGCGGTGCCGTTTTCACGTAc > 2:464954/1‑76 (MQ=255) cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa > 2:3414413/1‑76 (MQ=255) gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt > 1:27359/1‑76 (MQ=255) ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa < 1:3464760/76‑1 (MQ=255) gAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg < 1:4810779/76‑1 (MQ=255) aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg > 2:877791/1‑76 (MQ=255) aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt > 1:4957248/1‑76 (MQ=255) tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg > 2:4816125/1‑76 (MQ=255) tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg < 2:1361906/76‑1 (MQ=255) accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa > 1:4360334/1‑76 (MQ=255) ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAg < 1:1657777/76‑1 (MQ=255) cacaGACACTCGGTGCTGTCTTCCCAGAGTTGGCGAGTGAAGACCGCGTTGTCGTTTTCAAGTACATAGGTGAggg < 2:4522072/74‑1 (MQ=255) accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg < 1:3766216/76‑1 (MQ=255) ccaTACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGCCCGCGGTGTCGTTTTCAAGTACCTAGGTGAGGGc < 1:5019799/76‑1 (MQ=255) ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGc < 2:1550570/76‑1 (MQ=255) ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGc < 2:5243770/76‑1 (MQ=255) caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg < 2:839847/76‑1 (MQ=255) caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg < 2:4355124/76‑1 (MQ=255) aGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGGGAGGGCGa > 1:2226531/1‑76 (MQ=255) aGACACTCGGTGCGGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGa > 1:1568580/1‑76 (MQ=255) acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc < 1:2383163/76‑1 (MQ=255) acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc < 2:4360333/76‑1 (MQ=255) acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc < 1:388862/76‑1 (MQ=255) acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGACGACCGCGGTGTCGTTTTCAAGTACATAGGTGACGGCGATCAg > 2:1255017/1‑76 (MQ=255) acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg > 2:2093021/1‑76 (MQ=255) tctgTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt < 2:1575054/73‑1 (MQ=255) tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt < 1:725957/76‑1 (MQ=255) tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt < 1:5644322/76‑1 (MQ=255) tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt > 1:1942989/1‑76 (MQ=255) tCGGTGCTGTCTTCCCACAGGTGGCGAGTGAAGACCGCGGTGTCGTCTTCAAGTACCTCGGTGAGGGCGATCAggg > 1:3232493/1‑75 (MQ=255) cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGtt < 1:671497/76‑1 (MQ=255) ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg > 1:3784231/1‑76 (MQ=255) ggTGCTGTCTTCCCACAGTGGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg < 2:3420160/76‑1 (MQ=255) gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg > 1:5461004/1‑76 (MQ=255) gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg > 1:2615053/1‑76 (MQ=255) gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg > 1:2035244/1‑76 (MQ=255) gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg < 1:107978/76‑1 (MQ=255) tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt > 1:2127728/1‑76 (MQ=255) gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg > 2:690822/1‑76 (MQ=255) ctgcCTACCCACAGTCGGCGAGTGAAGACCGCGGTGTCGTTATCAAGCACATTGGGGAGGGAGACCAGGAtggggg > 1:1772604/1‑76 (MQ=255) | TCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG > NC_000913/4091279‑4091423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |