| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,316,203 | T→A | Q47L (CAG→CTG) | phnK ← | ATP‑binding component of phosphonate transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,316,203 | 0 | T | A | 99.3% | 419.0 / NA | 142 | Q47L (CAG→CTG) | phnK | ATP‑binding component of phosphonate transport |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (62/78); total (63/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 146 total aligned reads displayed.
GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA > NC_000913/4316131‑4316274 | gCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg < 2:2069587/76‑1 (MQ=255) cATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt > 1:1054824/1‑76 (MQ=255) gtACAGCGAGCGGGTCTCGGAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg < 2:635114/75‑1 (MQ=255) aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg < 1:2842153/76‑1 (MQ=255) cAGCGAACGGTTCTCGTAGAGAATTTCCCCCTGCTGCGGCGTAAGGCGCGCGGCGCTCGACGTCAGCTGCGCGGta > 1:1898637/1‑75 (MQ=255) aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCt < 2:4143142/76‑1 (MQ=255) gCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCtt < 1:2098075/76‑1 (MQ=255) cGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc > 1:4172928/1‑76 (MQ=255) gAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc < 2:1974378/76‑1 (MQ=255) gccGGTGCTCGTGGTGAATTCCCCCCGGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc < 2:5618306/74‑1 (MQ=255) aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCg < 2:3622622/76‑1 (MQ=255) cGGTTCTGGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg < 2:5817163/76‑1 (MQ=255) cGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACGTCAGCAGCGTGGTCTTCCCgg > 1:4870673/1‑76 (MQ=255) cGGTTCGCGTAGTGATTTTCCCCCTGCTGCGGCGTCATGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg < 2:4099124/76‑1 (MQ=255) ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGa < 1:682061/76‑1 (MQ=255) ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGa < 1:5841042/76‑1 (MQ=255) gTTCTCTTAGTGAATTTCCCCCCGCGGCGACGTCAGTCGCGCGGATATCGACTTCATCAGCGAGGTCTTCCCGGAg < 1:5335484/76‑1 (MQ=255) gTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg > 2:3522076/1‑76 (MQ=255) ttCTCGTAGTGAATTTCCCCCGGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc < 2:2361906/76‑1 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:4551541/76‑1 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:4091989/76‑1 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGcc > 2:2607666/1‑76 (MQ=255) ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCg > 2:316266/1‑76 (MQ=255) ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCg < 1:1175518/76‑1 (MQ=255) tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg > 1:2425412/1‑76 (MQ=255) tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg < 1:4811185/76‑1 (MQ=255) tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg > 1:5089972/1‑76 (MQ=255) tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg < 1:352279/76‑1 (MQ=255) tcGTAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg < 2:1264024/76‑1 (MQ=255) cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa < 1:5494245/76‑1 (MQ=255) cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa > 1:2549947/1‑76 (MQ=255) tAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAtt < 1:1030026/76‑1 (MQ=255) tAGTGAAGTGCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAtt < 2:1049674/76‑1 (MQ=255) aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGcc > 2:5004796/1‑70 (MQ=255) aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:5004797/70‑1 (MQ=255) aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTc > 1:2277115/1‑76 (MQ=255) gactttCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc < 1:1903803/73‑1 (MQ=255) gAATCTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGGCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc < 2:4789057/76‑1 (MQ=255) tttCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc < 2:1054824/76‑1 (MQ=255) tttCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc > 1:2828926/1‑76 (MQ=255) tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGAGTGGCCTTCCCGGAGCCGGACTCCACcacac > 2:5846732/1‑75 (MQ=255) ccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg > 1:1891303/1‑76 (MQ=255) cccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGc < 1:4187763/76‑1 (MQ=255) ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc < 2:4479419/76‑1 (MQ=255) ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc > 1:170234/1‑76 (MQ=255) ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc > 2:3010121/1‑76 (MQ=255) tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcccc > 2:2864225/1‑75 (MQ=255) tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcccc > 2:2472650/1‑75 (MQ=255) tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa > 1:5833879/1‑76 (MQ=255) tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa < 1:3526481/76‑1 (MQ=255) gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg < 1:528787/76‑1 (MQ=255) tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa < 1:3112020/76‑1 (MQ=255) tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa < 2:5054939/76‑1 (MQ=255) tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa > 1:4626686/1‑76 (MQ=255) gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc > 2:3943318/1‑76 (MQ=255) gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc > 2:4841570/1‑76 (MQ=255) gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc > 2:661523/1‑76 (MQ=255) gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc < 1:618617/76‑1 (MQ=255) cggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt < 1:682483/76‑1 (MQ=255) cggcgTCAGGCGCGCGGAGATCGACGTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt < 2:4953887/76‑1 (MQ=255) ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtt > 2:4022012/1‑76 (MQ=255) ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtt > 2:3146315/1‑76 (MQ=255) ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtt < 1:1874974/76‑1 (MQ=255) ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtc > 2:1599327/1‑75 (MQ=255) gcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc < 1:895905/76‑1 (MQ=255) gcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc > 1:894087/1‑76 (MQ=255) cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc < 1:5808651/76‑1 (MQ=255) aGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCg < 2:3710674/76‑1 (MQ=255) ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCgg < 2:4810461/76‑1 (MQ=255) gcgcgcGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc > 2:4935608/1‑73 (MQ=255) gcgcgcGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc < 1:4935609/73‑1 (MQ=255) cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc < 1:1922676/76‑1 (MQ=255) cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc < 1:151388/76‑1 (MQ=255) gcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa < 1:1106563/76‑1 (MQ=255) gcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa > 1:2985410/1‑76 (MQ=255) cgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCCCTTCCCCCGGCCag > 2:5063939/1‑75 (MQ=255) cgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAt < 1:1459250/76‑1 (MQ=255) gcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa > 1:4361061/1‑76 (MQ=255) gcgGAGATCGACTTCAGCAGCGTGGTCTGCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa < 2:3400444/76‑1 (MQ=255) gggaTCGACTTCAGGAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCCGCACTTCCCCCGGCCATAAAt < 2:1774190/74‑1 (MQ=255) gagaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt > 1:20086/1‑76 (MQ=255) gagaTCAACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt < 2:2554819/76‑1 (MQ=255) gagaACGACGTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt < 2:542086/76‑1 (MQ=255) agaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATc > 1:1145026/1‑76 (MQ=255) gaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa < 1:2532292/76‑1 (MQ=255) gaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCAGCCATAAATCa < 1:2943710/76‑1 (MQ=255) aTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATACATCaa > 2:1483072/1‑76 (MQ=255) tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCCCTTCCCCCGGcc < 2:1280321/66‑1 (MQ=255) tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc > 1:1280321/1‑66 (MQ=255) tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaa < 1:4451810/76‑1 (MQ=255) tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaa > 1:750050/1‑76 (MQ=255) cGACTTCAGCAACGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGGTCTTCCCCCGGCCATAAATCaaaa < 1:5263758/76‑1 (MQ=255) gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag > 1:3952639/1‑76 (MQ=255) gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag < 2:3696213/76‑1 (MQ=255) gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag > 1:5165228/1‑76 (MQ=255) agttCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga < 2:4752296/74‑1 (MQ=255) aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaaca > 2:450312/1‑74 (MQ=255) cTTCAGCAGGGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAagag < 2:5270084/76‑1 (MQ=255) ttgagcagcGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga < 2:5541841/73‑1 (MQ=255) tCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAc > 1:5669596/1‑76 (MQ=255) | GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA > NC_000913/4316131‑4316274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |