Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,043,272 A→G S130G (AGT→GGT)  b1978 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,043,2720AG100.0% 364.9 / NA 131S130G (AGT→GGT) b1978hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/78);  total (53/78)

Only 100 of 131 total aligned reads displayed.

AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG  >  NC_000913/2043200‑2043343
                                                                        |                                                                       
aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                      <  1:4017908/76‑1 (MQ=255)
 gCGTTGCGGAACGTTTCGGTATTTCGGTGGCTGAGTGACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                     <  2:164214/76‑1 (MQ=255)
 gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                     <  1:2155923/76‑1 (MQ=255)
  cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtttt                                                                    >  1:1172367/1‑76 (MQ=255)
   gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTg                                                                   >  1:5068465/1‑76 (MQ=255)
    ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGa                                                                  >  1:3195012/1‑76 (MQ=255)
    ttGCCGAACGGTGCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGa                                                                  >  1:3176371/1‑76 (MQ=255)
     tGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCACCCAGTTTCGTACGTTTGCTCGAGGTTTTGAt                                                                 <  1:675777/76‑1 (MQ=255)
      gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                <  2:5578808/76‑1 (MQ=255)
      gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                <  2:5327179/76‑1 (MQ=255)
      gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                >  1:1438461/1‑76 (MQ=255)
        cggaCGTTTCGGTATTTCGGGGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                              <  2:5839805/73‑1 (MQ=255)
        cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                              >  1:1290352/1‑76 (MQ=255)
         gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGTTTTTGATactt                                                             <  1:1479786/76‑4 (MQ=255)
          aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGt                                                            <  1:5814465/76‑1 (MQ=255)
           aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTc                                                           >  1:1084834/1‑76 (MQ=255)
              tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                        >  1:5073698/1‑76 (MQ=255)
              tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                        >  2:4481584/1‑76 (MQ=255)
               ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAGCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                       <  2:4128941/76‑1 (MQ=255)
               ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                       >  1:4505262/1‑76 (MQ=255)
                tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCa                                                      <  2:1226854/76‑1 (MQ=255)
                tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCa                                                      >  1:222385/1‑76 (MQ=255)
                 tggTATTTCGGTGGGTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                     <  2:363926/75‑1 (MQ=255)
                 cgttATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                     <  2:2221260/73‑1 (MQ=255)
                 cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                     <  2:72286/76‑1 (MQ=255)
                 cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                     >  1:3010254/1‑76 (MQ=255)
                 cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                     >  2:4947997/1‑76 (MQ=255)
                  ggTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                    >  2:5692911/1‑76 (MQ=255)
                    tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                  <  1:5758173/76‑1 (MQ=255)
                    tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                  <  1:5680944/76‑1 (MQ=255)
                    tATTGCGGTGGCTGAGTTACGCAAACTCAGCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                  <  1:5470284/76‑1 (MQ=255)
                     ttttCGGTGGCTGAGTGACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                 <  2:1763475/75‑1 (MQ=255)
                     aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                 <  1:4460122/76‑1 (MQ=255)
                     aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                 <  1:2537661/76‑1 (MQ=255)
                     aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGCTTGCTCGAGGTTTTGATAATGTCCGCCAgggcg                                                 >  1:5192970/1‑74 (MQ=255)
                      tttCGTTGGCTGAGTGACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtga                                                <  1:4620680/76‑1 (MQ=255)
                       ttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgat                                               <  2:3180767/76‑1 (MQ=255)
                       tgcGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgat                                               <  2:5120826/74‑1 (MQ=255)
                           gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                           <  2:394688/76‑1 (MQ=255)
                            tGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                          >  2:2092219/1‑76 (MQ=255)
                              gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                        <  1:2717250/76‑1 (MQ=255)
                              gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                        <  2:1802767/76‑1 (MQ=255)
                              gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                        <  2:2889236/76‑1 (MQ=255)
                                tGAGTTACGCAAACTCACCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAt                                      <  2:579423/76‑1 (MQ=255)
                                tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAt                                      <  1:3619598/76‑1 (MQ=255)
                                tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAt                                      <  1:669622/76‑1 (MQ=255)
                                  aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGt                                    <  2:2355370/76‑1 (MQ=255)
                                  aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGt                                    >  2:3425279/1‑76 (MQ=255)
                                  aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGt                                    >  1:5247474/1‑76 (MQ=255)
                                  aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGt                                    <  2:5232471/76‑1 (MQ=255)
                                   ggtACGCAAACTCAAACAGCTGCGGACGGTTGATCGAGGTTTTGATAATGTCAGCCAGGGTGATGAACTGGATGTc                                   >  1:1556091/3‑76 (MQ=255)
                                   gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTc                                   <  2:3693698/76‑1 (MQ=255)
                                   gTTACGCAAACTCAACCAGTTTCGGACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTc                                   >  2:2983722/1‑76 (MQ=255)
                                    ttACGCAAACTCAACCAGTTTTGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                  <  1:1844122/76‑1 (MQ=255)
                                    ttACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                  <  2:70871/76‑1 (MQ=255)
                                       cGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCgg                               <  2:4759647/76‑1 (MQ=255)
                                        gCAAACTCATCCAGTTTCGGACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                              <  2:5702276/76‑1 (MQ=255)
                                        gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                              <  1:5702192/76‑1 (MQ=255)
                                         cAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGca                             <  1:3404479/76‑1 (MQ=255)
                                          aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                            >  2:1199184/1‑76 (MQ=255)
                                          aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                            >  1:2914601/1‑76 (MQ=255)
                                          aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                            >  1:5567038/1‑76 (MQ=255)
                                           aaCGCAACCAGTTGCGTTCGTTTGCTCGAGGGTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                           <  2:311134/76‑1 (MQ=255)
                                            aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCCCaa                          >  2:1495759/1‑76 (MQ=255)
                                            aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                          <  1:4680000/76‑1 (MQ=255)
                                             cTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAg                         <  1:1300694/76‑1 (MQ=255)
                                              tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCTCGGGTGATGAACTGGATGTCCCGGCACAAGt                        >  1:2059429/1‑76 (MQ=255)
                                              tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                        >  2:5079657/1‑76 (MQ=255)
                                              tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                        <  1:2983722/76‑1 (MQ=255)
                                              tCAACCAGTTGCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                        <  2:5847200/76‑1 (MQ=255)
                                              tCAACCAGTGTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAgc                        >  1:4562169/1‑75 (MQ=255)
                                               cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGtt                       <  2:4505261/76‑1 (MQ=255)
                                                aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                      >  2:2158775/1‑76 (MQ=255)
                                                aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                      <  1:337903/76‑1 (MQ=255)
                                                  ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACCAGTTCGt                    >  2:5651737/1‑76 (MQ=255)
                                                  ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                    <  2:2188105/76‑1 (MQ=255)
                                                  ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                    <  2:5073697/76‑1 (MQ=255)
                                                   cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                   <  1:5208717/76‑1 (MQ=255)
                                                   cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGCCCCGGCACAAGTTACTg                   >  2:5728089/1‑76 (MQ=255)
                                                    aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGa                  <  2:436290/76‑1 (MQ=255)
                                                       ttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaa               <  2:5262663/76‑1 (MQ=255)
                                                       ttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaa               >  1:4619918/1‑76 (MQ=255)
                                                        tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa              <  2:2850034/76‑1 (MQ=255)
                                                        tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa              >  1:1995818/1‑76 (MQ=255)
                                                         cGTACGTTTGGTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa             <  2:5842517/76‑1 (MQ=255)
                                                         cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACACGTTAGTGaaaaaa             <  1:2344911/76‑1 (MQ=255)
                                                         cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa             <  2:4187451/76‑1 (MQ=255)
                                                          gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaa            >  2:4811697/1‑76 (MQ=255)
                                                           tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa           <  2:3290741/76‑1 (MQ=255)
                                                            aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt          <  2:3010254/76‑1 (MQ=255)
                                                             cGTTTGCTCGAGGTTTTGAGAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAtt         <  2:451450/76‑1 (MQ=255)
                                                              gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa        >  1:1685428/1‑76 (MQ=255)
                                                              gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa        >  1:774259/1‑76 (MQ=255)
                                                              gTTTGCTCCAGGCTTTGATAATGTCCGCCAGTGTGATGAACTGGATGTCCCGGCACAAGTGTGTGGAAAAAAAATa        >  1:1716793/1‑76 (MQ=255)
                                                                 tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAACATTAAcc     >  2:3932413/1‑76 (MQ=255)
                                                                 tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc     <  1:3321643/76‑1 (MQ=255)
                                                                 tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc     <  1:4063256/76‑1 (MQ=255)
                                                                 tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc     >  2:5230779/1‑76 (MQ=255)
                                                                   cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc   <  2:5654313/76‑1 (MQ=255)
                                                                    tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccg  <  1:577957/76‑1 (MQ=255)
                                                                        |                                                                       
AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG  >  NC_000913/2043200‑2043343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑