| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,072,030 | T→A | C824S (TGC→AGC) | b2000 → | outer membrane fluffing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,072,030 | 0 | T | A | 97.4% | 417.1 / NA | 156 | C824S (TGC→AGC) | b2000 | outer membrane fluffing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (72/79); minor base C (4/0); total (76/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 161 total aligned reads displayed.
TGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCC > NC_000913/2071957‑2072102 | tgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGc > 2:1209008/1‑76 (MQ=255) gctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGGGCCGGCACGGTCCGGGGTGATGCCGgccgcc > 2:4835175/1‑76 (MQ=255) gctgGCCATTCCTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc < 1:1688959/76‑1 (MQ=255) ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt < 1:3622874/76‑1 (MQ=255) ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt < 1:5011062/76‑1 (MQ=255) ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt < 1:3806937/76‑1 (MQ=255) tgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTg > 2:4730275/1‑76 (MQ=255) gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg < 1:3999001/76‑1 (MQ=255) gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg > 1:965091/1‑76 (MQ=255) gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTggg < 1:2068392/76‑1 (MQ=255) ccATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGc > 2:4578392/1‑76 (MQ=255) ccATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGc < 1:3318333/76‑1 (MQ=255) cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg > 1:5328838/1‑76 (MQ=255) cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg < 1:2897150/76‑1 (MQ=255) cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg > 2:2231103/1‑76 (MQ=255) aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg > 1:1566255/1‑76 (MQ=255) aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCATGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg < 1:3595259/76‑1 (MQ=255) tcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGAt < 1:140490/76‑1 (MQ=255) cttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATa < 2:2400893/76‑1 (MQ=255) cttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCCGGGCGGGTa > 2:3866783/1‑76 (MQ=255) ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCCGCCCGGGCGGATAc > 2:2043029/1‑76 (MQ=255) tcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAcc > 1:5546171/1‑76 (MQ=255) ccGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCt > 1:4106121/1‑76 (MQ=255) cGTTGATGTTAGGCATGAGGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATGCCTg < 1:1545117/76‑1 (MQ=255) cGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTg > 1:5159731/1‑76 (MQ=255) cGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTg < 2:2470750/76‑1 (MQ=255) gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCCGCCTGGGCGGATCCCTgc > 2:4059805/1‑75 (MQ=255) gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa > 1:949797/1‑76 (MQ=255) ttGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGACACCTCaa > 1:2121119/1‑76 (MQ=255) ttGATGTTAAGGATGATCACGGCTCCCGTGCCGGCACGGCCCGGGATGATACCCGCACCCTTGGCGGATCCCTgta > 2:2865349/1‑74 (MQ=255) tggtGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAt < 2:5346947/73‑1 (MQ=255) gATGTTAAGGATGATGACGGCTCCCGTGCCGGCCCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGACTc > 2:1549974/1‑76 (MQ=255) gATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATc > 1:2443136/1‑76 (MQ=255) aTGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCt < 1:4952758/76‑1 (MQ=255) tGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTg > 1:679219/1‑76 (MQ=255) tGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTg < 1:3824339/76‑1 (MQ=255) aaGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTac < 2:5332306/76‑1 (MQ=255) aaGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTac > 1:4081443/1‑76 (MQ=255) aGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTaca < 1:2000712/76‑1 (MQ=255) ggATGGTGACGGCTGCCGTGGCAGCTGGGTCCGGGATCATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacgc < 2:1509463/76‑3 (MQ=255) ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacac > 1:2917639/1‑76 (MQ=255) ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacac < 1:3145755/76‑1 (MQ=255) gatgatGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca < 1:5306853/76‑1 (MQ=255) gatgatGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca < 1:225488/76‑1 (MQ=255) gatgatGACGGCGCCCGTGCCGGCGCGGTCCGGGATGATGCCGGCAGCGTGGGCGGATACCTGAATCTGGTacaca < 2:2858492/76‑1 (MQ=255) atgatgACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac < 1:3719551/76‑1 (MQ=255) atgatgACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCCGGGCGGGTACCCGGATCTGGTacccac > 2:1423173/1‑76 (MQ=255) gatggCGGCGCCGGTGCCGGCCCGGTGCGGGATGATGGCGGCAGCTTGGGCGGATACCTGAATCTTGTACACCCGt < 2:2309773/76‑1 (MQ=255) gtgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtc < 2:4923658/75‑1 (MQ=255) tgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc < 1:427161/76‑1 (MQ=255) taCGGCGCGCGTGGCGGCACGGTCCGGGAGGATGCCGGCAGCCTGGGCGGATCCCTGAATCTGGTACACACGtcct < 1:5649978/75‑1 (MQ=255) gaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcct > 1:3144916/1‑76 (MQ=255) aCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctc < 1:4313225/76‑1 (MQ=255) cGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc < 2:1846988/76‑1 (MQ=255) cGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc < 1:1209008/76‑1 (MQ=255) ggCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCg > 1:3507904/1‑76 (MQ=255) ggCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGACGGATACCTGAATCTGGTACACACGTCCTCCg < 2:3098345/76‑1 (MQ=255) gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCgg > 1:4534815/1‑76 (MQ=255) gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGACACCTGAATCTGGTACACACGgccccccg > 2:5433444/1‑74 (MQ=255) cTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGc < 2:3200314/76‑1 (MQ=255) tCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGcc > 2:4893649/1‑76 (MQ=255) tCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGGACACACGTCCTCCGGcc > 2:4686763/1‑76 (MQ=255) tCCCGTGCCGGCACGGCCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGcc > 2:5667921/1‑76 (MQ=255) ccccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATCCCTGAATCTGGTACACACGTCCTCCGGcc < 1:1857434/75‑1 (MQ=255) ccccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGcc < 1:336005/75‑1 (MQ=255) cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGccc > 2:3981765/1‑75 (MQ=255) cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCt < 1:2519506/76‑1 (MQ=255) cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCt > 1:2362287/1‑76 (MQ=255) ccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtg > 2:4688290/1‑76 (MQ=255) cGTGCCCGCACGGTCCGGGATGATGCCGGCAGCCCGGGCGGACACCTGAATCTGATAGACACGTCCTCCGGCCtct > 2:4360081/1‑74 (MQ=255) gTGCCGGCACGGTCCGGGATGATGCCGGCCGCCTGGGCGGATACCTGAATCTGGTACACACGACCTCCGGGTtgtg > 2:412885/1‑76 (MQ=255) gTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgtg > 1:30059/1‑76 (MQ=255) ccGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGc > 2:3226069/1‑76 (MQ=255) cGGCACGGTGCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt < 2:3283271/76‑1 (MQ=255) cGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt < 2:2575471/76‑1 (MQ=255) cACGGTCCGGGATGATGCCGGCAGCCTGGGCGGCTACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTCAc > 2:5600701/1‑76 (MQ=255) ggTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAtt > 1:128955/1‑76 (MQ=255) gccGGAAGGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgt < 1:5714224/75‑1 (MQ=255) ccGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgtg < 2:4071059/76‑1 (MQ=255) ccGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgt > 2:3506545/1‑75 (MQ=255) cGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgg > 1:2835885/1‑76 (MQ=255) cGGGAAGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCAGGCCTGTGGGCTGACATTGTgg > 1:5620984/1‑76 (MQ=255) gggATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGc > 1:4181526/1‑76 (MQ=255) ggATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGca > 2:1148309/1‑76 (MQ=255) gatgatGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGcac > 1:5758437/1‑76 (MQ=255) atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGCCATTGTGGcaca < 1:1698354/76‑1 (MQ=255) atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGcaca > 1:4722433/1‑76 (MQ=255) atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGcaca < 1:2885831/76‑1 (MQ=255) tgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAg < 1:647537/76‑1 (MQ=255) gatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg < 1:4688291/76‑1 (MQ=255) gatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg > 1:2805360/1‑76 (MQ=255) gatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg < 1:1353793/76‑1 (MQ=255) ccGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGCCATTTTGGCACAGGGCAc > 2:5792281/1‑76 (MQ=255) cGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAcc > 2:3002650/1‑76 (MQ=255) ggcggcCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc < 2:3841355/76‑1 (MQ=255) ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc < 1:3730607/76‑1 (MQ=255) ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc < 1:803603/76‑1 (MQ=255) ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc < 2:1403568/76‑1 (MQ=255) ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc < 1:1327491/76‑1 (MQ=255) ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGACGCACAGGCAcccc > 2:518669/1‑76 (MQ=255) | TGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCC > NC_000913/2071957‑2072102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |