| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,216,746 | G→C | S252S (TCC→TCG) | yehZ ← | putative transport system permease protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,216,746 | 0 | G | C | 99.3% | 363.2 / NA | 136 | S252S (TCC→TCG) | yehZ | putative transport system permease protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (60/75); minor base T (0/1); total (60/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCC > NC_000913/2216674‑2216818 | cAATGTTTTTGCATCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAc < 1:301388/76‑1 (MQ=255) aTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc < 1:2497721/76‑1 (MQ=255) aTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc < 1:3262380/76‑1 (MQ=255) aTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGGAGGCACTGTGCCCTTTTCGGGTAATCCCCCAAAACCCACTc > 1:4714655/1‑76 (MQ=255) tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCa < 1:3295410/76‑1 (MQ=255) gTTTTTGCATCGAGGCTGGCGAAGACTGGCTGGAGCCACCGTGCCACTTTCCGGTACTCCCCCCACCCCCACTCAc > 2:1968546/1‑76 (MQ=255) ttggCATCGGGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGca < 2:4878741/73‑1 (MQ=255) gCAGCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATCCTCCCTCAACACCGACTCACGctcca < 1:4737889/76‑1 (MQ=255) tCGAGGCTGGCGAAGACTGGCTGGAGCCCCCGTGCCCTTTTTGGGTACTCCCCCAACACCCACTCACCcaccaccg > 1:1881865/1‑74 (MQ=255) gAGGCTGGCGAAGACTGGCTGTAGGCCCTGTGGCCTTTTCGGGTACTCCCCCCACAACCACCCCCCCACCACTggg > 2:3597140/1‑75 (MQ=255) gAGGCTGGCGAAGACTGGCTGGGGGCCCTGTGGCCTTTTTGGGTAATCCCTCAACAACGCCCCACGCACCACCGGt > 1:4356928/1‑76 (MQ=255) tggCTGGCGAAGACTGTCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTg < 1:1534013/75‑1 (MQ=255) ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGc < 2:3895370/76‑1 (MQ=255) gCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCa < 2:2015884/76‑1 (MQ=255) cTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAg < 1:4407709/76‑1 (MQ=255) cTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAg < 1:4394229/76‑1 (MQ=255) tGGCGAAGACTGGCTGGAGCCACTGTGCCCTTTGCGGGTACTCCCTCCACACCGGCTCCCGCACCACCGGGGCAgg > 1:3693126/1‑76 (MQ=255) ggCGACGACTGGCTGTAGCCACTGGGCCATTTGCGGATACTCCCCCCACACCGACCCCCCCCGCCCTGGCGCAGgc > 2:4342034/1‑76 (MQ=255) ggCGAAGACTGGCTGTAGCCACTGTGCCATTTTCGGATACTCCCCCCACACCCACTCACGCACCACCGGGGCAGgc > 1:1442439/1‑76 (MQ=255) ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgc < 1:2622953/76‑1 (MQ=255) ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgc < 1:5689272/76‑1 (MQ=255) ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgc < 1:2260347/76‑1 (MQ=255) ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgc < 1:305826/76‑1 (MQ=255) gggAAGACGGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcg < 2:5440389/74‑1 (MQ=255) gCGAAGACTAGCTGTAGCCACTGTGCCATTTGCGGATACTCCCCCAACCCCGACCCACCCACCCCCGGGGCAAgcc > 2:755763/1‑75 (MQ=255) cGAAGACTGGGTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcgc < 2:2954481/76‑1 (MQ=255) cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACCCCCCCTCCCGCCCCACCCGCGCAGgcgc > 2:2582669/1‑76 (MQ=255) cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcgc < 1:4408580/76‑1 (MQ=255) cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcgc < 1:4138351/76‑1 (MQ=255) gAAGACTGGCTGTAGCCACTGTGCCATTTTCGGATACTCCCCCAACACCCACCCCCGCACCCCCGGTGCCAGcgcg > 2:1588383/1‑76 (MQ=255) gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGGTACTCCCTCAACCCCCCCCCACGCCCCACCGGTGCAGGcgcg > 1:69594/1‑76 (MQ=255) gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCCACTCACGCACCACCGGTGCAGGcgcg > 1:2737427/1‑76 (MQ=255) gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCCACTCACGCACCACCCGCGCAGGcgcg > 1:2737842/1‑76 (MQ=255) gAAGACTGGCTGGAGCCACTGTGCCCTTTGCGGATACTCCCTCAACACCGACCCACCCACCACCGGGGCAGGcgcg > 2:1121658/1‑76 (MQ=255) aaNACTGGCGGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGt < 2:2548525/76‑1 (MQ=255) aaGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGt < 1:5058455/76‑1 (MQ=255) gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAg > 1:3895610/1‑76 (MQ=255) tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa > 1:2297019/1‑76 (MQ=255) tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGCAGATa > 1:1004201/1‑76 (MQ=255) gggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa < 1:668015/75‑1 (MQ=255) ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAg > 1:3623708/1‑76 (MQ=255) cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt > 1:4052831/1‑76 (MQ=255) cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt < 1:4722420/76‑1 (MQ=255) cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt > 1:2960829/1‑76 (MQ=255) cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt > 2:858360/1‑76 (MQ=255) tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt > 2:4628860/1‑76 (MQ=255) gTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTg < 1:4519824/76‑1 (MQ=255) gTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTg < 2:3512965/76‑1 (MQ=255) tAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGc < 1:3489400/76‑1 (MQ=255) tAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGc < 2:3794976/76‑1 (MQ=255) aGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca > 2:4499972/1‑76 (MQ=255) gCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcac > 1:794003/1‑76 (MQ=255) gCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcac < 1:508225/76‑1 (MQ=255) ccACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcaca < 2:5386527/76‑1 (MQ=255) ccACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcaca > 1:3998675/1‑76 (MQ=255) cgcTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac < 1:3204358/74‑1 (MQ=255) cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGTCGCGTAGATAGGTTGCacac < 1:2083832/76‑1 (MQ=255) cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGCTAGGTTGCacac > 2:1994883/1‑76 (MQ=255) cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac < 2:5602302/76‑1 (MQ=255) cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac > 1:2780009/1‑76 (MQ=255) cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac < 1:4130353/76‑1 (MQ=255) aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc > 2:5184154/1‑76 (MQ=255) aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc > 2:2740610/1‑76 (MQ=255) tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt < 1:372430/76‑1 (MQ=255) tgtgCCATTTGCGGATACTCCCTCAACACCGAATCACGCCCCACTGGTGAAGGCGCGTAGATAGCTTGCACACCtt > 2:2286938/1‑76 (MQ=255) gtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg < 1:4575707/76‑1 (MQ=255) tgCCATTGGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc < 1:126423/76‑1 (MQ=255) gCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCg < 1:580654/76‑1 (MQ=255) ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg < 1:1470816/76‑1 (MQ=255) ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg < 1:3819059/76‑1 (MQ=255) ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg < 1:1949840/76‑1 (MQ=255) ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTCGCgg > 2:5332435/1‑76 (MQ=255) cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa < 2:1960065/76‑1 (MQ=255) cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa > 1:3480015/1‑76 (MQ=255) tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGc > 2:4840258/1‑76 (MQ=255) cGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCtt < 1:3675782/76‑1 (MQ=255) ggATACTCCCCCAACCCCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa < 2:5648351/76‑1 (MQ=255) gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa > 2:2326751/1‑76 (MQ=255) gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa > 1:4358893/1‑76 (MQ=255) gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa < 2:3975646/76‑1 (MQ=255) tACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg < 1:5155279/76‑1 (MQ=255) tACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg > 2:1507233/1‑76 (MQ=255) tACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg > 1:4906794/1‑76 (MQ=255) cTCCCTCAACGCCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt < 2:1599069/76‑1 (MQ=255) cTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt < 2:2296929/76‑1 (MQ=255) cTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt < 2:2538440/76‑1 (MQ=255) tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt < 1:5408250/76‑1 (MQ=255) tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt > 2:3699161/1‑76 (MQ=255) tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt > 2:3114581/1‑76 (MQ=255) cccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTTCACACCTTGCGGATCGCTTAAGGTTTg < 2:4659645/76‑1 (MQ=255) cccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTg < 2:4920295/76‑1 (MQ=255) ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc < 1:4532543/76‑1 (MQ=255) ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc < 2:3138673/76‑1 (MQ=255) ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc < 1:1336688/76‑1 (MQ=255) cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa < 1:1937861/76‑1 (MQ=255) cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa > 2:4357993/1‑76 (MQ=255) tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAg > 1:388350/1‑76 (MQ=255) cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc > 2:1836686/1‑76 (MQ=255) acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc > 2:3991159/1‑76 (MQ=255) cacTGACTCACGCGCCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcccc < 1:3556385/76‑1 (MQ=255) | CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCC > NC_000913/2216674‑2216818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |