BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,915	A→G	K21E (AAG→GAG)	srlA →	PTS system, glucitol/sorbitol‑specific IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,915	0	A	G	100.0%	338.9 / NA	125	K21E (AAG→GAG)	srlA	PTS system, glucitol/sorbitol‑specific IIC component
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (78/47); total (78/47)

Only 100 of 125 total aligned reads displayed.

AAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTG  >  NC_000913/2823843‑2823986

aaGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                      >  1:4425398/1‑76 (MQ=255)

 aGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCACAAGGGCGGAGAggg                                                                     >  2:5816651/1‑75 (MQ=255)

 aGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAggg                                                                     >  2:2519614/1‑75 (MQ=255)

  ggAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGgtg                                                                    >  2:2708247/1‑76 (MQ=255)

    agagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGtt                                                                  <  1:5643333/76‑1 (MQ=255)

      agaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTa                                                                >  2:5470500/1‑76 (MQ=255)

      agaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTa                                                                <  1:1516566/76‑1 (MQ=255)

            aTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGa                                                          >  2:5841783/1‑76 (MQ=255)

             tGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGAt                                                         <  1:4847074/76‑1 (MQ=255)

              gATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                                        >  1:2382642/1‑76 (MQ=255)

              gATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGCTTACCGGGATg                                                        >  2:419629/1‑76 (MQ=255)

                 aGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTg                                                     >  1:4431642/1‑76 (MQ=255)

                  gAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAc                                                               <  1:359316/65‑1 (MQ=255)

                   aaaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                   >  2:3047790/1‑76 (MQ=255)

                   aaaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                   >  2:3359446/1‑76 (MQ=255)

                    aaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTCCCGGGATGGTGccc                                                  >  2:3079595/1‑76 (MQ=255)

                    aaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAcc                                                  >  2:3061604/1‑76 (MQ=255)

                    aaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAcc                                                  >  2:4216127/1‑76 (MQ=255)

                        aTTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                              >  1:2149382/1‑76 (MQ=255)

                         ttACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                             >  1:3808620/1‑76 (MQ=255)

                         ttACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                             >  2:1432972/1‑76 (MQ=255)

                         ttACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                             >  1:3286653/1‑76 (MQ=255)

                         ttACTCATGGGGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                             >  2:1000394/1‑76 (MQ=255)

                          tACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAtt                                            >  2:5288757/1‑76 (MQ=255)

                          tACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAtt                                            >  2:169616/1‑76 (MQ=255)

                           aCTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttc                                           <  1:5223794/76‑1 (MQ=255)

                            cTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                          <  1:5057692/76‑1 (MQ=255)

                            cTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                          <  1:783541/76‑1 (MQ=255)

                            cTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                          >  1:1167200/1‑76 (MQ=255)

                            cTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCCGAGAGGTGTTTACCGCGATGGTGACCGCCTttct                                          >  2:5591826/1‑76 (MQ=255)

                            atCATGGTGCAGAGTGGTTTATCGGGCTGTTTCAAAAGGGCGGAGAGGTGTGTACCGGGATGGCGACCGGCAttct                                          >  1:5273879/2‑76 (MQ=255)

                               aTGGTGCAGCGTGGTTTATCGGGCTGTTCCAGCAGGGCGGCGATGTGTTTAGGGGGGTGAAGACCGGCCttctccc                                       <  2:3415026/76‑4 (MQ=255)

                               aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTcc                                       <  2:3543857/76‑1 (MQ=255)

                               aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTcc                                       <  1:1907763/76‑1 (MQ=255)

                                tGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCg                                      >  2:4425042/1‑76 (MQ=255)

                                  gTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCt                                    <  2:843422/76‑1 (MQ=255)

                                    gCAGAGTGGTTTATCGGGTTGTTCCAAAAGGGCGGAGACGTGTTTACGGGGATGGGCACCGGCCTTCTTCCACtct                                  >  2:158359/1‑74 (MQ=255)

                                    gCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGt                                  >  1:3612414/1‑76 (MQ=255)

                                    gCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGt                                  <  2:726181/76‑1 (MQ=255)

                                    gCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGt                                  <  2:4387055/76‑1 (MQ=255)

                                    gCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGt                                  <  1:1792039/76‑1 (MQ=255)

                                     cAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGtt                                 >  2:4553108/1‑76 (MQ=255)

                                     cAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGtt                                 <  1:4309371/76‑1 (MQ=255)

                                      agagTGGTTTATCGGTCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                <  2:3306305/76‑1 (MQ=255)

                                      agagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                <  1:5092240/76‑1 (MQ=255)

                                      agagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                <  1:169616/76‑1 (MQ=255)

                                      agagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                <  2:2770392/76‑1 (MQ=255)

                                       gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGa                               <  2:1380071/76‑1 (MQ=255)

                                       gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGa                               >  1:2790207/1‑76 (MQ=255)

                                        agTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAt                              <  2:153956/76‑1 (MQ=255)

                                         gTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAtt                             >  1:4122379/1‑76 (MQ=255)

                                          tGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTa                            >  1:3232809/1‑76 (MQ=255)

                                            gTTTATCGGGCTGTTCCACAAGCGCGGAGAGGTCTTTACCGGGCTGGTGCCCGGAATTCTTCCGCCGATGATTCGc                          >  2:647258/1‑76 (MQ=255)

                                            gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGAGTTTACCGGGATGCTGACCGGCATTCTTCCGCTGTTGATTAGc                          >  1:3956196/1‑76 (MQ=255)

                                             tttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGcc                         <  1:2257855/76‑1 (MQ=255)

                                              ttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCct                        <  2:3818659/76‑1 (MQ=255)

                                              ttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCct                        >  2:128074/1‑76 (MQ=255)

                                               tATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctg                       <  1:5499415/76‑1 (MQ=255)

                                               tATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctg                       >  2:4796454/1‑76 (MQ=255)

                                               tATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctg                       >  1:4795371/1‑76 (MQ=255)

                                                aTCGGGCTGTTGCAAAAGGTGGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                      <  1:612902/76‑1 (MQ=255)

                                                aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                      >  2:4152442/1‑76 (MQ=255)

                                                aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                      >  1:1979938/1‑76 (MQ=255)

                                                aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                      >  1:2922443/1‑76 (MQ=255)

                                                 tCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgct                     <  1:128074/76‑1 (MQ=255)

                                                 tCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgct                     <  2:3740309/76‑1 (MQ=255)

                                                  cGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgctg                    >  2:2104561/1‑76 (MQ=255)

                                                  cGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgctg                    <  1:3244158/76‑1 (MQ=255)

                                                  cGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgctg                    <  1:3130647/76‑1 (MQ=255)

                                                   gggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGCTTAGCCTGCTgg                   >  1:1077813/1‑76 (MQ=255)

                                                   gggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTgg                   >  1:2563393/1‑76 (MQ=255)

                                                   gggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTgg                   <  1:5323819/76‑1 (MQ=255)

                                                   gggCTGTTCCAAAAGGGCGGAGAGGCGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTgg                   >  2:4026472/1‑76 (MQ=255)

                                                    ggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGt                  >  1:195179/1‑76 (MQ=255)

                                                    ggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGt                  <  2:2790207/76‑1 (MQ=255)

                                                     gCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGtt                 >  2:1995575/1‑76 (MQ=255)

                                                     gCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGtt                 >  1:3487892/1‑76 (MQ=255)

                                                      cTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTa                >  2:3699887/1‑76 (MQ=255)

                                                       tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt               <  1:783456/76‑1 (MQ=255)

                                                       tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt               >  2:639368/1‑76 (MQ=255)

                                                       tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt               >  1:694749/1‑76 (MQ=255)

                                                         ttCCAAAAGGGCGGAGAGGCGTTTACCGGGATGGTGACCGGTATGCTTGCGCTGTTGATTAGCGCGCTGGTGATCa             >  1:4853055/1‑76 (MQ=255)

                                                           ccAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg           <  1:44675/76‑1 (MQ=255)

                                                           ccAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg           >  2:2019863/1‑76 (MQ=255)

                                                           ccAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg           <  2:1158393/76‑1 (MQ=255)

                                                           ccAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg           <  2:1266986/76‑1 (MQ=255)

                                                            cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt               >  1:1425127/1‑71 (MQ=255)

                                                            cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt               <  2:1419813/71‑1 (MQ=255)

                                                            cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt               >  1:1419813/1‑71 (MQ=255)

                                                               aaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACg       <  1:4289973/76‑1 (MQ=255)

                                                               aaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACg       >  2:2037643/1‑76 (MQ=255)

                                                                 gggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCa     <  2:4972398/76‑1 (MQ=255)

                                                                 gggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCa     >  2:4747583/1‑76 (MQ=255)

                                                                 gggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCa     >  2:4185380/1‑76 (MQ=255)

                                                                  ggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc    >  1:1314500/1‑76 (MQ=255)

                                                                  ggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc    >  1:181242/1‑76 (MQ=255)

                                                                  ggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc    >  1:1847603/1‑76 (MQ=255)

                                                                   gCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACt   >  2:2998420/1‑76 (MQ=255)

                                                                    cGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTg  <  1:5148873/76‑1 (MQ=255)

                                                                    cGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTg  >  2:805446/1‑76 (MQ=255)

AAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTG  >  NC_000913/2823843‑2823986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`