BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,797	T→C	F128S (TTT→TCT)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,797	0	T	C	100.0%	394.9 / NA	142	F128S (TTT→TCT)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (79/63); total (79/63)

Only 100 of 142 total aligned reads displayed.

TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG  >  NC_000913/2824725‑2824867

tgGAATATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACATCCCTCTTc                                                                     <  1:1608049/76‑1 (MQ=255)

tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                     <  2:4919447/76‑1 (MQ=255)

 ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                    <  1:2554728/76‑1 (MQ=255)

  gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                   >  2:1276163/1‑76 (MQ=255)

  gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                   >  2:2995738/1‑76 (MQ=255)

  gAAGATATTGATGTCTCGGGAGTAAAAGAACAAAAAATAACTGTAGGAGCAGAGGCGACACCACAACCCTCTGCCg                                                                   >  2:938550/1‑76 (MQ=255)

   aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                  <  1:2447015/76‑1 (MQ=255)

   aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                  >  2:338045/1‑76 (MQ=255)

   aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGCAGGCGATGCGACACCACAACCCTCTTCCGt                                                                  >  2:2342933/1‑76 (MQ=255)

    aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                 >  1:4732887/1‑76 (MQ=255)

    aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                 <  1:3128943/76‑1 (MQ=255)

     gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                >  2:1844586/1‑76 (MQ=255)

     gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                >  1:2554445/1‑76 (MQ=255)

     gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                <  2:2914412/76‑1 (MQ=255)

      atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                               <  2:4780770/76‑1 (MQ=255)

      atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                               <  2:2650907/76‑1 (MQ=255)

         tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                            >  1:3745082/1‑76 (MQ=255)

          ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                           >  1:978317/1‑76 (MQ=255)

            aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                         <  2:1406955/76‑1 (MQ=255)

             tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTTCGTGGGCCGTGAc                                                        >  1:5074358/1‑76 (MQ=255)

                 tgtGGTGTAAACTAAGAAAACATCGCTGTCGTCGGTCACGGGCCTCCGCACCCCTCTTCGGTGGGCCGTGACTATg                                                    <  2:3381034/74‑1 (MQ=255)

                 tctGGGGTAAAAGAAGAAAACATCGCTGTGGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                    <  2:199394/76‑1 (MQ=255)

                 tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                    >  1:1099461/1‑76 (MQ=255)

                 tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                    >  2:330825/1‑76 (MQ=255)

                 tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                    >  2:1102235/1‑76 (MQ=255)

                 tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                    >  1:2650732/1‑76 (MQ=255)

                 tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGCGCCGTGACTATg                                                    >  1:5141380/1‑76 (MQ=255)

                  ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                   <  2:2132973/76‑1 (MQ=255)

                   tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                  >  1:5308821/1‑76 (MQ=255)

                     gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                >  2:5709679/1‑76 (MQ=255)

                      cGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACTCCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                               <  1:432904/76‑1 (MQ=255)

                       gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                              <  2:3473072/76‑1 (MQ=255)

                        tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                             <  1:5275211/76‑1 (MQ=255)

                        tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                             <  1:2251223/76‑1 (MQ=255)

                        tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                             >  2:5521527/1‑76 (MQ=255)

                         aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAga                                            >  1:611816/1‑75 (MQ=255)

                         aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                            >  1:3436147/1‑76 (MQ=255)

                          aaaGAAGAAAACATGACTGTAGTAGGTGATTCGACACCACAACCCTCTTCCGGTGGCGGTGCGTATGACACCAgca                                           >  2:3829791/1‑74 (MQ=255)

                          aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                           >  2:3823120/1‑76 (MQ=255)

                           aacaagaaAACATCACTGAAGTAGGTGATGCGACACCACAACCCTCATCCGTGAGCCGAGACTATGACACCAGTaa                                          >  2:4435035/4‑76 (MQ=255)

                            agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                             <  1:1472110/72‑1 (MQ=255)

                            agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                         <  1:1955137/76‑1 (MQ=255)

                             gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                        <  1:1588121/76‑1 (MQ=255)

                             gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                        <  1:2759547/76‑1 (MQ=255)

                             gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                        <  1:5432141/76‑1 (MQ=255)

                              aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                       <  1:2827946/76‑1 (MQ=255)

                              aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                       <  1:3859204/76‑1 (MQ=255)

                              aagaaAACAGCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                       <  2:358956/76‑1 (MQ=255)

                               agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                      >  1:3730400/1‑76 (MQ=255)

                               agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                      <  1:2885038/76‑1 (MQ=255)

                               agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                      <  1:84891/76‑1 (MQ=255)

                               agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGCAAGaaa                                      >  2:1627058/1‑76 (MQ=255)

                               agaaAACATCACTGTAGTAGGGGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                      >  1:4837695/1‑76 (MQ=255)

                                 aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                    <  1:4263054/76‑1 (MQ=255)

                                   aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                  >  1:3445557/1‑76 (MQ=255)

                                   aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                  <  2:4485940/76‑1 (MQ=255)

                                      aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                               <  1:3178861/76‑1 (MQ=255)

                                       tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACCCCAGTAAGAAAATCACCGa                              >  2:410551/1‑76 (MQ=255)

                                       tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGa                              >  2:3513170/1‑76 (MQ=255)

                                        cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCCGTAAGAAAACCACCGaa                             >  2:47791/1‑76 (MQ=255)

                                        cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                             <  1:5144230/76‑1 (MQ=255)

                                        cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                             >  1:176648/1‑76 (MQ=255)

                                         aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                            >  2:2103617/1‑76 (MQ=255)

                                         aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                            <  2:2509468/76‑1 (MQ=255)

                                         aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                            >  1:5537191/1‑76 (MQ=255)

                                          cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                           >  2:3439648/1‑76 (MQ=255)

                                           tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTCTGGCACCAGTAAGAAAATCACCGAACaa                          <  2:1159343/76‑1 (MQ=255)

                                           tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                          >  2:5261884/1‑76 (MQ=255)

                                           tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                          >  1:3237280/1‑76 (MQ=255)

                                           tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                          <  2:3851165/76‑1 (MQ=255)

                                            gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                         >  1:5116887/1‑76 (MQ=255)

                                             tagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                        <  2:680436/76‑1 (MQ=255)

                                              agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                       <  2:2567235/76‑1 (MQ=255)

                                               gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                      >  1:3846138/1‑76 (MQ=255)

                                                tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                     >  2:2388807/1‑76 (MQ=255)

                                                 agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                    <  1:1194130/76‑1 (MQ=255)

                                                  ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                   <  1:529308/76‑1 (MQ=255)

                                                   gTGATGCGACACGACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                  <  1:4522311/76‑1 (MQ=255)

                                                   gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                  <  2:1296481/76‑1 (MQ=255)

                                                   gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                  >  1:308376/1‑76 (MQ=255)

                                                    tGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                 >  1:1030095/1‑76 (MQ=255)

                                                     gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                >  1:4762923/1‑76 (MQ=255)

                                                     gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                >  1:1789784/1‑76 (MQ=255)

                                                     gATGCGACACCACAACCCTCTGCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGCACAAAGCGATGGtt                >  1:2286419/1‑76 (MQ=255)

                                                        gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGCACAAAGCGATGGTTTat             >  2:1386384/1‑75 (MQ=255)

                                                        gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc             >  1:4367632/1‑76 (MQ=255)

                                                        gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc             >  2:1311832/1‑76 (MQ=255)

                                                        gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc             <  1:2809157/76‑1 (MQ=255)

                                                         cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt            >  1:766152/1‑76 (MQ=255)

                                                          gACACCACAACCCTCTTCCGTGGGCCGTGCCTATGCCACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg           >  1:4534948/1‑76 (MQ=255)

                                                          gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                         >  2:5196938/1‑62 (MQ=255)

                                                           acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg          <  1:1482407/76‑1 (MQ=255)

                                                              ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGa       <  1:3744678/76‑1 (MQ=255)

                                                               cacaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGTCGaa      <  2:4896687/76‑1 (MQ=255)

                                                                acaGCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg     <  2:1530005/76‑1 (MQ=255)

                                                                acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg     >  2:4032950/1‑76 (MQ=255)

                                                                 caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAggg    <  2:5474475/76‑1 (MQ=255)

                                                                  aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGACggg   >  2:44913/1‑75 (MQ=255)

                                                                  aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt   <  1:5521529/76‑1 (MQ=255)

                                                                   aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg  <  2:1849555/76‑1 (MQ=255)

TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG  >  NC_000913/2824725‑2824867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`