Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,130,334 A→G V30V (GTT→GTC)  b2983 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,130,3340AG97.9% 377.8 / NA 143V30V (GTT→GTC) b2983hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (57/83);  minor base C (3/0);  total (60/83)
Fisher's exact test for biased strand distribution p-value = 7.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG  >  NC_000913/3130262‑3130406
                                                                        |                                                                        
tACGCCAAACATGGCGGGCGTCATCCCTTTACCCGCACACGAGAGCGCCCACAGACCCAGCAAGGCGCTCacgaca                                                                       >  2:548199/1‑76 (MQ=255)
 aCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCCACAGACCCCGCAAGGCGCTCACGACAt                                                                      >  1:26372/1‑76 (MQ=255)
 aCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                      <  1:4535870/76‑1 (MQ=255)
  cGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAtt                                                                     <  2:2355573/76‑1 (MQ=255)
   gcaaaaCATGGCGGGCTTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTa                                                                    <  1:2766405/73‑1 (MQ=255)
   gCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTa                                                                    <  1:2045614/76‑1 (MQ=255)
   gCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCACCAAGGCCCTCacgacactc                                                                    >  2:4088861/1‑73 (MQ=255)
    ccAAACATGGGGGGTGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGGCCCCGCACGGCGCTCACGACCTTAc                                                                   <  2:634595/76‑1 (MQ=255)
    ccAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTAc                                                                   <  2:4503721/76‑1 (MQ=255)
    ccAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACCACACTAc                                                                   >  2:1758363/1‑76 (MQ=255)
      agaCAGGGCGGGCGTCATCCCTTTGCCGGCACACGAGAGCGCCAACAGGCCCAGCAAGGCGCTCACGACATTACTg                                                                 <  2:1754963/74‑1 (MQ=255)
      aaaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                 >  1:4727638/1‑76 (MQ=255)
      aaaCATAGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                 >  2:2895619/1‑76 (MQ=255)
       acgaTGGCGGGCGCCACCCCTTTGCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTgg                                                                <  2:3512107/73‑1 (MQ=255)
        aCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCACGGCGCTCACGACATTACTGGa                                                               >  2:5298558/1‑76 (MQ=255)
        aCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGa                                                               <  1:508995/76‑1 (MQ=255)
         cATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                              <  1:1288352/76‑1 (MQ=255)
          agggcgcgcgCCACCCCTTTACCGGCACAGGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAGTACTGGAGc                                                             <  2:125731/74‑1 (MQ=255)
          aTGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGc                                                             >  1:3103762/1‑76 (MQ=255)
           tgtcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGcc                                                            <  1:5301524/73‑1 (MQ=255)
           tGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGcc                                                            <  2:3502332/76‑1 (MQ=255)
            ggcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCCGCAACGCGCTCACGACATTCCTGGAGCCt                                                           >  1:2166073/1‑76 (MQ=255)
            ggcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCTAGGCGCTCACGACATTACTGGAGACt                                                           >  2:2046011/1‑76 (MQ=255)
             gcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTa                                                          <  1:452030/76‑1 (MQ=255)
             gcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTa                                                          >  2:2581782/1‑76 (MQ=255)
              cgggcgTCAGCCGTTTACCGGCGCGCGATAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTaa                                                         <  1:1770287/76‑1 (MQ=255)
               gggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAc                                                        >  1:1789581/1‑76 (MQ=255)
                 gcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCa                                                      <  1:2356101/76‑1 (MQ=255)
                   gTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAgg                                                    >  1:427276/1‑76 (MQ=255)
                     tgtCCCTTTCCCGGCGCACGAGAGCGCTAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcg                                                  <  1:353957/74‑1 (MQ=255)
                     cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcg                                                  >  1:43602/1‑76 (MQ=255)
                      ctCCCTTTACCGGCACGCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                 <  2:2717692/75‑1 (MQ=255)
                      aTCCCTTTACCGGCACACGAGAGCGCCAACCGACCCAGCACGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                 >  1:1270573/1‑76 (MQ=255)
                      aTCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                 >  2:1160601/1‑76 (MQ=255)
                      aTCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCACgcgc                                                 >  2:1842513/1‑76 (MQ=255)
                      aTCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGAAAGGCGCTCACGACATTACTGGGGCCTTACCTGgcgc                                                 >  2:3464103/1‑76 (MQ=255)
                         ccTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                              >  2:4737970/1‑76 (MQ=255)
                         ccTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                              >  1:5153159/1‑76 (MQ=255)
                            ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                           >  1:4098144/1‑76 (MQ=255)
                             tACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCg                                          >  2:5209905/1‑76 (MQ=255)
                             tACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCg                                          >  2:3238484/1‑76 (MQ=255)
                              cccGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGa                                         <  1:1403469/75‑1 (MQ=255)
                              aCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGa                                         <  1:927605/76‑1 (MQ=255)
                              aCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCCAACCACGCGCCACTGcca                                         >  2:201119/1‑74 (MQ=255)
                               ccGGCACATGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                        <  1:4903094/76‑1 (MQ=255)
                               ccGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                        >  1:3652439/1‑76 (MQ=255)
                                cGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                       <  1:2099946/76‑1 (MQ=255)
                                 ggCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                      >  1:4893549/1‑76 (MQ=255)
                                  gCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                     <  1:4799632/76‑1 (MQ=255)
                                   cacaCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                    >  1:4576354/1‑76 (MQ=255)
                                    acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTGACCAGGCGCTATTGCGAATAATg                                   <  2:4506987/76‑1 (MQ=255)
                                     cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                  >  1:5154192/1‑76 (MQ=255)
                                     cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                  >  1:1712535/1‑76 (MQ=255)
                                       cGAGATCGCCAACAGACCCAGCTAGGCGCTCGCGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                <  2:4622943/76‑1 (MQ=255)
                                       cGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                <  1:3238484/76‑1 (MQ=255)
                                       cGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                <  2:3641121/76‑1 (MQ=255)
                                        gggagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                               <  2:601439/74‑1 (MQ=255)
                                        gagaGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                               <  2:30246/76‑1 (MQ=255)
                                        gagaGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGAGc                               >  1:651005/1‑76 (MQ=255)
                                        gagaGCGCCAACAGACCCAGCAAGGCGCGCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                               <  2:3105570/76‑1 (MQ=255)
                                            gcgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATACTGGTGCGaaa                           >  2:1545632/1‑76 (MQ=255)
                                            gcgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                           >  2:3721706/1‑76 (MQ=255)
                                            gcgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                           <  2:43602/76‑1 (MQ=255)
                                               ccAACAGACCCAGCAAGGCGCTCACGACATTACTGTAGCCTAACCAGGCGCTATTGCGAATAATGGTGCTAAAcgc                        <  1:5282417/76‑1 (MQ=255)
                                               ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgc                        >  2:901321/1‑76 (MQ=255)
                                               ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgc                        <  1:2618018/76‑1 (MQ=255)
                                               ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgc                        <  1:2212517/76‑1 (MQ=255)
                                                cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                         >  2:2718333/1‑74 (MQ=255)
                                                cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                         <  1:2718333/74‑1 (MQ=255)
                                                cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCCCTATCGCGACTAATGCCGCGAAAcgcc                       >  2:5474369/1‑75 (MQ=255)
                                                 aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACgcgc                      >  2:4724992/1‑76 (MQ=255)
                                                 aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACgcgc                      <  2:3103762/76‑1 (MQ=255)
                                                 aaCAGACCCAGCAAGGCGCTCACGACATTACGGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACgcgc                      <  1:4540201/76‑1 (MQ=255)
                                                  aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                     <  1:668586/76‑1 (MQ=255)
                                                  aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                     <  2:4743811/76‑1 (MQ=255)
                                                  aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                     <  1:2938323/76‑1 (MQ=255)
                                                   cAGACCCGGGAAGGCGCTCACGCCCTTCCTGGAGCCTACCCAGTGGCTCTTGCGAATAATGGTGCGCACCGCGccc                    <  2:5366845/76‑2 (MQ=255)
                                                    aGACCCAGCAGGGCGCTCACGACATTCCTGGAGCCTACCCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAt                   <  1:1135872/76‑1 (MQ=255)
                                                      gcccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                 <  1:853419/75‑1 (MQ=255)
                                                       cccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                <  1:2246805/76‑1 (MQ=255)
                                                       cccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                >  2:4243521/1‑76 (MQ=255)
                                                       cccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                >  1:3527487/1‑76 (MQ=255)
                                                        ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGc               >  2:3343037/1‑76 (MQ=255)
                                                        ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGc               <  1:5128190/76‑1 (MQ=255)
                                                        ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGc               >  2:2071038/1‑76 (MQ=255)
                                                         cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa              <  2:855394/76‑1 (MQ=255)
                                                          aGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa             <  2:5356717/76‑1 (MQ=255)
                                                            cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAc           <  2:1828580/76‑1 (MQ=255)
                                                             aaGGCGCTCACGACATTATTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAcc          >  1:3429787/1‑76 (MQ=255)
                                                              aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCa         <  1:4382877/76‑1 (MQ=255)
                                                              aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAACa         >  1:845268/1‑76 (MQ=255)
                                                                gcgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt       >  1:3953408/1‑76 (MQ=255)
                                                                gcgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGAGAAGAATGCTGCGAAACGCGCCATCTGCAAACCAAt       >  1:488351/1‑76 (MQ=255)
                                                                 cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCCATg      >  2:436567/1‑76 (MQ=255)
                                                                 cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg      >  1:2131769/1‑76 (MQ=255)
                                                                  gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt     <  1:3222440/76‑1 (MQ=255)
                                                                  gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt     <  1:4243522/76‑1 (MQ=255)
                                                                    tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt   <  1:3626645/76‑1 (MQ=255)
                                                                    tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT‑GTGCGAAACGCGCCATCTGCAAACCAATGTTTg  <  2:4610440/76‑1 (MQ=255)
                                                                     cacgACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg  <  2:1582964/76‑1 (MQ=255)
                                                                        |                                                                        
TACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG  >  NC_000913/3130262‑3130406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑