| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,279,197 | A→C | R145S (AGA→AGC) | agaA → | putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,279,197 | 0 | A | C | 99.4% | 435.4 / NA | 165 | R145S (AGA→AGC) | agaA | putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (67/96); total (68/97) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 168 total aligned reads displayed.
CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG > NC_000913/3279124‑3279269 | cGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt > 1:3477568/1‑76 (MQ=255) gTCTCTGCATCGTGCGCGATTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc < 1:5414069/76‑1 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:5429651/1‑76 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc < 1:3025789/76‑1 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 1:3253538/1‑76 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 1:2671132/1‑76 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 1:3836685/1‑76 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc < 1:89589/76‑1 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:2928461/1‑76 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:3184477/1‑76 (MQ=255) gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:5100407/1‑76 (MQ=255) tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCg < 1:5497898/76‑1 (MQ=255) cgatGCATCCGGCGCGCATGCTGGGTGTTGATGGTGTTCTGGGAGCGATTAAACCGGGCCAACGCGCCAGcgtcgt > 1:4931495/4‑76 (MQ=255) gCTGCATCCGGCGCGAATGCTGGGTGATGATGGTGTTCTGGGTGCGCTTAAACGGGGCACACGCGCGAGAGTCCtt > 1:5639484/1‑76 (MQ=255) tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc > 1:3801678/1‑76 (MQ=255) cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc > 1:1654284/1‑76 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 2:4753236/76‑1 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt > 1:3275899/1‑76 (MQ=255) tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTCgcgccg > 2:838764/1‑74 (MQ=255) ccGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg > 1:929979/1‑76 (MQ=255) cGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa < 1:5139855/76‑1 (MQ=255) cGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa < 1:279167/76‑1 (MQ=255) ggCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt < 1:434838/76‑1 (MQ=255) ggCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt > 2:3420016/1‑76 (MQ=255) gcgcGAATGATGGGTGTTGATGTTGGTCTGTGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa < 1:3714540/76‑1 (MQ=255) cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCTCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg < 1:3707636/76‑1 (MQ=255) cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg < 1:3593969/76‑1 (MQ=255) tgtaaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACAGGGCAAACTCGCCAGCGTCGTTGCGCTGGATAGc < 1:1674981/73‑1 (MQ=255) gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc < 1:3686834/76‑1 (MQ=255) gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc < 2:1209479/76‑1 (MQ=255) gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGGCGTTGCGCTGGATAGc > 2:1871400/1‑76 (MQ=255) gcgAATGCTGGGCGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc > 2:1627998/1‑76 (MQ=255) cgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGCATAGCg > 2:1427422/1‑76 (MQ=255) gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 2:4693082/76‑1 (MQ=255) aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc > 2:3885449/1‑76 (MQ=255) aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc < 2:1680500/76‑1 (MQ=255) aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc < 1:1078500/76‑1 (MQ=255) gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 2:3544001/1‑76 (MQ=255) gCTGGGTGTTGATGGTGTACTGGTATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa < 2:4188872/76‑1 (MQ=255) gCTGGGTGTTGATGGGGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 2:4664713/1‑76 (MQ=255) cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc < 2:4145309/76‑1 (MQ=255) cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc < 1:3719832/76‑1 (MQ=255) gggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt < 2:5590522/76‑1 (MQ=255) ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg > 1:5567163/1‑76 (MQ=255) gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGc > 1:2611957/1‑76 (MQ=255) tGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTCGCGCTGGATAGCGGGCTACATGTGcaa > 2:3033058/1‑76 (MQ=255) gATGGTGTGCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac < 2:2603756/76‑1 (MQ=255) aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca < 1:3166573/76‑1 (MQ=255) aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca < 1:3002413/76‑1 (MQ=255) aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca > 1:2226288/1‑76 (MQ=255) tGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt < 2:5302266/55‑1 (MQ=255) gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCCACAAAt > 2:3685696/1‑76 (MQ=255) gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGGCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt > 2:4093684/1‑76 (MQ=255) tgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc < 1:4181399/76‑1 (MQ=255) gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt < 1:3838725/76‑1 (MQ=255) gtTCTGGGATCGCTTAAACCGAGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCGACATGCGCAACAAATCt > 2:1072791/1‑76 (MQ=255) ggtCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCGACATGGGCAACAAATCt > 2:4888162/3‑76 (MQ=255) ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg < 2:1474577/76‑1 (MQ=255) ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg < 2:4651902/76‑1 (MQ=255) tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg < 1:527255/76‑1 (MQ=255) cgggggTCGCTGAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa < 2:63716/74‑1 (MQ=255) cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa > 1:582298/1‑76 (MQ=255) tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAt > 2:2890778/1‑76 (MQ=255) gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt > 2:2876660/1‑76 (MQ=255) ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc < 1:2442766/76‑1 (MQ=255) ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc < 2:4476564/76‑1 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 1:1229457/76‑1 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa > 1:5450494/1‑76 (MQ=255) tCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAgg < 2:4773428/76‑1 (MQ=255) cGCTTGAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg < 1:700035/76‑1 (MQ=255) cGCTTAAACCGGGCAAACGCGCCGGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg < 2:2226288/76‑1 (MQ=255) gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt < 1:2984527/76‑1 (MQ=255) catAACCCGGGGAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc < 1:2867463/74‑1 (MQ=255) cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGTATTCAGGGTc < 1:1145487/76‑1 (MQ=255) cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc < 2:2487981/76‑1 (MQ=255) cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc < 2:5575086/76‑1 (MQ=255) ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 1:457604/76‑1 (MQ=255) ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 2:4923652/76‑1 (MQ=255) ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa > 2:3804061/1‑76 (MQ=255) aCCGGGGCAACTCGCGAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCCGGGTCAATTa < 1:1281257/76‑1 (MQ=255) aCCGGGCACACGCGCCAGCGTCGTTGCGCTGGCTCGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAttc < 2:3808900/76‑2 (MQ=255) aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa > 1:4087760/1‑76 (MQ=255) aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa < 1:1871400/76‑1 (MQ=255) gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt > 1:3205976/1‑76 (MQ=255) gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt > 1:2266445/1‑76 (MQ=255) ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCCGGGTCAATTAGCtt > 2:2047010/1‑76 (MQ=255) gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc > 1:1081060/1‑76 (MQ=255) gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc > 1:5698540/1‑76 (MQ=255) gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc > 2:5229802/1‑76 (MQ=255) cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg > 1:2180747/1‑76 (MQ=255) cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg < 2:4957286/76‑1 (MQ=255) cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg < 1:1235415/76‑1 (MQ=255) aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGt > 2:215149/1‑76 (MQ=255) aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGt < 2:3450277/76‑1 (MQ=255) aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt > 2:4286987/1‑76 (MQ=255) aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt < 1:4666356/76‑1 (MQ=255) cgcgCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtttt > 2:461603/1‑76 (MQ=255) gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt > 1:4534236/1‑76 (MQ=255) ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa < 2:3274781/76‑1 (MQ=255) cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAg < 2:2804083/76‑1 (MQ=255) | CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG > NC_000913/3279124‑3279269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |