| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,714,436 | 2 bp→GC | coding (261‑262/660 nt) | yiaD → | putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,714,436 | 0 | C | G | 100.0% | 356.7 / NA | 133 | N87K (AAC→AAG) | yiaD | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (60/73); total (60/73) | |||||||||||
| * | NC_000913 | 3,714,437 | 0 | G | C | 99.2% | 330.5 / NA | 132 | V88L (GTG→CTG) | yiaD | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (58/73); minor base A (1/0); total (59/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 136 total aligned reads displayed.
TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC > NC_000913/3714366‑3714506 || aTTGGCGCGGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTAGATGGATGTGCAGGAAGCGAAGCTGcgcg < 2:4674253/76‑1 (MQ=255) aTTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGcgcg < 2:4444068/76‑1 (MQ=255) ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGa < 2:719917/76‑1 (MQ=255) tGGCGCAGCAGCAGGCGCAGCTCTGGGGGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAc < 2:715594/76‑1 (MQ=255) tGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTCCATGGATGTGCCGGAAGCGAAGCTGcgcgcc < 2:2261011/76‑3 (MQ=255) tGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAc > 1:4850976/1‑76 (MQ=255) ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGGGACa > 2:2578619/1‑76 (MQ=255) cgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGACGCTGCGCGACaaa > 2:2042788/1‑76 (MQ=255) gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa < 2:1726977/76‑1 (MQ=255) gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTACTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa > 1:2859523/1‑76 (MQ=255) agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACAAGGATGTGGAGGAAGCGACGCTGCGCGGCaaaaac > 2:458713/1‑74 (MQ=255) gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc > 1:1307599/1‑76 (MQ=255) gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc > 1:2740729/1‑76 (MQ=255) cagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg < 1:1888121/76‑1 (MQ=255) ngcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGAGAAAAAATtcgg > 1:2347168/2‑75 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc > 1:4726199/1‑76 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc < 1:3204734/76‑1 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc < 1:5527334/76‑1 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc < 1:1100157/76‑1 (MQ=255) cagGCGCAGCTCTGGGCGGCTNCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg > 1:4516472/1‑76 (MQ=255) agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc > 1:1686904/1‑76 (MQ=255) agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc > 2:2489906/1‑76 (MQ=255) agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc < 2:3572198/76‑1 (MQ=255) agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc > 2:4180984/1‑76 (MQ=255) ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa < 2:3857185/76‑1 (MQ=255) gcgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc > 1:5822404/1‑76 (MQ=255) cgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACt < 1:3785811/76‑1 (MQ=255) cgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACt < 2:2928342/76‑1 (MQ=255) cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTgg < 1:763248/76‑1 (MQ=255) cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTgg < 1:4343306/76‑1 (MQ=255) aGCTCTGGGCGGCGGCGTTGGTTATTCCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt < 1:3446726/76‑1 (MQ=255) aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt > 1:2937011/1‑76 (MQ=255) ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtgt < 2:3919976/76‑1 (MQ=255) ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtgt > 1:2727400/1‑76 (MQ=255) tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt > 1:2759500/1‑76 (MQ=255) tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg < 2:4544855/76‑1 (MQ=255) ggggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg < 2:919446/75‑1 (MQ=255) gcggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCCCCGGTGTTAGCGt > 2:5270229/1‑76 (MQ=255) gcggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt < 1:4326932/76‑1 (MQ=255) gcggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt < 2:3869454/76‑1 (MQ=255) gcggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt < 2:5466089/76‑1 (MQ=255) ggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTaa > 1:5747634/1‑76 (MQ=255) gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc > 2:3238568/1‑76 (MQ=255) gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc > 1:4529230/1‑76 (MQ=255) gggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc < 2:2545051/75‑1 (MQ=255) cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc < 2:1336058/76‑1 (MQ=255) cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc < 1:3665598/76‑1 (MQ=255) cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc < 2:3807000/76‑1 (MQ=255) cggcgTTGGGTATTACATCGATGTGCAGGAAGAGACGCCGCGCGACAAAACGCGCGGCACTGGTCACAGCGTAAcc > 2:2411976/1‑76 (MQ=255) ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCTCGGCACTGGTGTTAGCGTAAccc > 1:5523188/1‑76 (MQ=255) ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc > 1:367104/1‑76 (MQ=255) gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg < 1:411427/76‑1 (MQ=255) gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg < 2:4409287/76‑1 (MQ=255) gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg < 1:2785507/76‑1 (MQ=255) ggTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc < 2:4842883/75‑1 (MQ=255) cgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc < 2:1027725/76‑1 (MQ=255) cgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc < 1:2028765/76‑1 (MQ=255) cgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc < 1:2809486/76‑1 (MQ=255) gTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa < 2:3053606/76‑1 (MQ=255) ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg < 2:611777/76‑1 (MQ=255) ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg > 2:3145571/1‑76 (MQ=255) tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg < 1:821469/72‑1 (MQ=255) tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc < 2:2232800/76‑1 (MQ=255) tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc < 1:1562759/76‑1 (MQ=255) gggtattaCAGGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg < 2:923024/73‑1 (MQ=255) gTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg < 1:4345038/76‑1 (MQ=255) gTTATGACAGGGATGGGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg < 2:5757478/76‑1 (MQ=255) ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCggg < 2:2349928/76‑1 (MQ=255) ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCggg < 2:4850975/76‑1 (MQ=255) ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCggg > 1:3572894/1‑76 (MQ=255) ttattaCATGGATATGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCggg < 1:2190019/76‑1 (MQ=255) tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg > 2:4777950/1‑76 (MQ=255) ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt < 2:2225122/76‑1 (MQ=255) ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt < 1:3847316/76‑1 (MQ=255) ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGCGTTAGCGGCACCCGCAGCggggct > 2:410591/1‑74 (MQ=255) taCGTGGATGTGCAGGAAGCGAAGCCGCGCGCCAAAATGCGCGGCGCTGGTGTTAGCGTATCCCGCAGCGGGGATa < 2:1762506/76‑1 (MQ=255) taCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGGGTTAGCGGCACCCGCAGCggggcga > 2:450876/1‑73 (MQ=255) aCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa > 2:1967431/1‑76 (MQ=255) aCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa < 1:3521683/76‑1 (MQ=255) cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAc > 1:2013242/1‑76 (MQ=255) tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAt > 2:2801262/1‑76 (MQ=255) tGGAGGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAt < 2:543019/76‑1 (MQ=255) gggATGTGCGGGCAGCGAAGCTTCGCGACAAAATGCGCGTCACTGGTGTTAGCGTCACCCGCAGCGTGGATAACAt < 2:1391089/75‑1 (MQ=255) gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa > 1:3773489/1‑76 (MQ=255) gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa > 1:1401890/1‑76 (MQ=255) gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa < 1:4158002/76‑1 (MQ=255) tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc > 1:5485695/1‑76 (MQ=255) tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc > 2:3377378/1‑76 (MQ=255) gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc < 2:5747633/76‑1 (MQ=255) gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc > 2:3065917/1‑76 (MQ=255) cAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGGTAGCGTAACCCGCAGCGGGGATACAATTATCCCCa > 2:5547530/1‑76 (MQ=255) aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa > 1:565387/1‑76 (MQ=255) aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa < 2:4146790/76‑1 (MQ=255) ggAAGCGAAGCTGCGCGACACAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat < 1:1744881/76‑1 (MQ=255) ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat < 2:5198356/76‑1 (MQ=255) ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat < 1:3795625/76‑1 (MQ=255) gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata > 1:4472492/1‑76 (MQ=255) gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGAGGGGATAACATTATCCTCAata > 1:2497395/1‑76 (MQ=255) aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat > 2:5177655/1‑76 (MQ=255) gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGc < 2:4299403/76‑1 (MQ=255) || TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC > NC_000913/3714366‑3714506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |