| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,737,956 | 2 bp→CG | coding (623‑624/1254 nt) | avtA → | alanine‑alpha‑ketoisovalerate transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,737,956 | 0 | G | C | 99.2% | 346.8 / ‑5.4 | 131 | G208A (GGC→GCG) | avtA | alanine‑alpha‑ketoisovalerate transaminase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (66/64); minor base T (1/0); total (67/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
| * | NC_000913 | 3,737,957 | 0 | C | G | 99.2% | 351.6 / ‑4.8 | 131 | G208A (GGC→GCG) | avtA | alanine‑alpha‑ketoisovalerate transaminase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (66/64); total (67/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA > NC_000913/3737886‑3738024 || ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa < 1:210598/76‑1 (MQ=255) ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 2:3739364/1‑76 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac > 1:4097049/1‑76 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGAGCTGGCGAATCaaa > 1:2880339/1‑75 (MQ=255) ccccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 1:3324685/75‑1 (MQ=255) cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaaac > 2:5385068/1‑74 (MQ=255) cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca < 1:918672/76‑1 (MQ=255) cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca > 1:3705204/1‑76 (MQ=255) ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 1:5630784/76‑1 (MQ=255) ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGATGAAGCTTGACGCGCTGGCGAATCAacac > 2:2995605/1‑76 (MQ=255) cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGGTGCTGAAGCTTGACGCGCTGGCGAATCAACACg > 1:5150302/1‑76 (MQ=255) ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg > 2:1855352/1‑76 (MQ=255) ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg > 1:1672184/1‑76 (MQ=255) ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg < 1:2247932/76‑1 (MQ=255) ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg < 2:4683454/76‑1 (MQ=255) gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 2:2058064/76‑1 (MQ=255) ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa > 1:5273091/1‑76 (MQ=255) cgccgaATGCAACAGGCCATCTTATTACTGACGAAGCGTTGCTGAATCTTGTCGCGCCGGCGCATCAACACGGCAt < 1:5252873/73‑1 (MQ=255) acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc > 2:1955825/1‑76 (MQ=255) acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc > 2:3326839/1‑76 (MQ=255) acgaATCCAAAAGGCAATGCGATTACTGACGAAGAGATGCTGAAGCATGACGCGCTCGCGAATCAACACGGCAATc > 1:1911764/1‑76 (MQ=255) cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATAAACACCGCATccc > 2:5733272/1‑76 (MQ=255) gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg > 1:5461096/1‑76 (MQ=255) gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg > 2:4344783/1‑76 (MQ=255) gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg > 2:5812822/1‑76 (MQ=255) aacccaccaGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTTACTCGCTGGCGGATCAACACGGCATTCCGc < 1:4305448/73‑1 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGATGCTTGAGGCGGTGGCGACTCACCACGGCATTCGGc < 2:4003174/76‑1 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCCTTCCGc > 2:3977670/1‑76 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt < 2:424392/71‑1 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc > 2:4932763/1‑76 (MQ=255) aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCt < 1:2737409/76‑1 (MQ=255) aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCt > 2:2085689/1‑76 (MQ=255) tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg < 1:640820/76‑1 (MQ=255) tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg < 1:231046/76‑1 (MQ=255) cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt < 1:1790268/76‑1 (MQ=255) cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt < 2:1488446/76‑1 (MQ=255) aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg > 2:3251987/1‑76 (MQ=255) aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg > 2:3247134/1‑76 (MQ=255) aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga < 1:5529878/76‑1 (MQ=255) aGGCAATGGGATTACTGACGAAGAGTGGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:263738/76‑1 (MQ=255) ggCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattg < 2:508383/76‑1 (MQ=255) gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga < 1:3748329/76‑1 (MQ=255) gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga > 2:5730382/1‑76 (MQ=255) cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat < 1:4207692/76‑1 (MQ=255) aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 2:3256989/1‑76 (MQ=255) aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 2:3697414/1‑76 (MQ=255) aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 1:977817/1‑76 (MQ=255) aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa > 1:4030296/1‑76 (MQ=255) aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa > 1:3219483/1‑76 (MQ=255) aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGCGGTTCATaa > 2:5580591/1‑76 (MQ=255) tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGGGATTGATaaa > 1:627291/1‑75 (MQ=255) gtgATTGCTGACGAAGAGTTGCTGAAGCTTTACGCGCTGGCGAATGAGCACGGCATTCCGCTGGTGATTGATAACg < 2:1378916/76‑1 (MQ=255) tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc < 2:2510926/76‑1 (MQ=255) aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt > 1:1312174/1‑76 (MQ=255) aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt > 2:1356593/1‑76 (MQ=255) aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt < 1:3329280/76‑1 (MQ=255) aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt < 1:1908966/76‑1 (MQ=255) ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCCTa > 2:452499/1‑76 (MQ=255) tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt < 1:3392200/76‑1 (MQ=255) tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt < 1:3326839/76‑1 (MQ=255) tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt < 1:4885132/76‑1 (MQ=255) tcTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg < 2:4633867/75‑1 (MQ=255) aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTCTg > 2:5843907/1‑76 (MQ=255) cTGACGAAGAGTTGCTCGAGCTTGACGAGCTTGTCAAGCGACACGGCATTCCGCTGGGGATTTATAACGCTTAttg > 1:1662048/1‑74 (MQ=255) tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc < 2:1622967/76‑1 (MQ=255) tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc < 1:3543214/76‑1 (MQ=255) ggcGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg < 2:5004043/74‑1 (MQ=255) aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 1:370603/1‑76 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc < 1:2467028/76‑1 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc > 2:4551717/1‑76 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc > 1:1455172/1‑76 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc > 2:620849/1‑76 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc > 2:1929151/1‑76 (MQ=255) agagTTGCTTAAGCTTGCCGCGCTGGCGACTCGACACGGCATTCCGCTGGTGCTTGATAACGCTTATGGCGTCCCg < 1:2010975/76‑1 (MQ=255) agagTTGCTGAAGCGTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccca < 2:3191183/76‑2 (MQ=255) gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt > 2:5056179/1‑76 (MQ=255) gagTTGCTGAACCTTGACGCGCTGTCGACTCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt < 1:3513154/76‑1 (MQ=255) agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt < 1:3626146/76‑1 (MQ=255) agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt > 2:1191065/1‑76 (MQ=255) gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc < 1:1245754/76‑1 (MQ=255) gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg < 1:3585519/76‑1 (MQ=255) cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg < 2:1312174/76‑1 (MQ=255) cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg < 2:4241114/76‑1 (MQ=255) tGAAGCTTGACGCGCTGGCGAATCAACACGGCCTTCCGCTGGTGAGTGATAACTCATATGGCGTCCCGTTACCggg > 1:3233422/1‑76 (MQ=255) tGAAGCTTGACGAGCTGGCGAATCAACACGGCATTCCGCTGGCGAGTGAGAACGCTTATGGCGACACGTTCCCcgg > 1:279579/1‑76 (MQ=255) gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt < 2:2414395/76‑1 (MQ=255) gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt < 1:86810/76‑1 (MQ=255) aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa < 2:4097048/76‑1 (MQ=255) aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTat > 1:4613989/1‑76 (MQ=255) cTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca < 2:2285789/76‑1 (MQ=255) ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat > 1:2762781/1‑76 (MQ=255) ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat > 2:2488018/1‑76 (MQ=255) ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat < 1:5642364/76‑1 (MQ=255) ctGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat < 2:1275734/75‑1 (MQ=255) gACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt > 1:2677149/1‑76 (MQ=255) gACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt < 2:4833801/76‑1 (MQ=255) aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCtt > 1:1566481/1‑76 (MQ=255) aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCtt < 1:2633240/76‑1 (MQ=255) cgcgcgGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc < 2:101569/76‑1 (MQ=255) gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa > 2:3627620/1‑76 (MQ=255) || GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA > NC_000913/3737886‑3738024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |