| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,950,557 | 2 bp→GC | coding (451‑452/930 nt) | ilvE → | branched‑chain amino‑acid aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,950,557 | 0 | C | G | 99.3% | 360.2 / NA | 138 | R151A (CGA→GCA) | ilvE | branched‑chain amino‑acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (68/69); total (68/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,950,558 | 0 | G | C | 100.0% | 368.7 / NA | 139 | R151A (CGA→GCA) | ilvE | branched‑chain amino‑acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (68/71); total (68/71) | |||||||||||
Only 100 of 139 total aligned reads displayed.
GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG > NC_000913/3950489‑3950626 || aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAac > 1:2875141/1‑76 (MQ=255) gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca > 1:1070187/1‑76 (MQ=255) gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca > 1:4186154/1‑76 (MQ=255) cGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac < 2:2622121/76‑1 (MQ=255) gTATCTGGGCGCAGCAGCGATGGAGCAGGGGATCGCTGCGAAGGCTCCCTCCTGGAACCGCGCTGCACCAAACAcc > 1:137469/1‑76 (MQ=255) gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc > 1:4136676/1‑76 (MQ=255) aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt > 2:4708520/1‑76 (MQ=255) cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc < 2:1699091/76‑1 (MQ=255) tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc < 2:3376113/76‑1 (MQ=255) ggggCGCACAAGCGCTGGAGCAGGGGATCGATGCGATAGTTTCCGCCTGGAACCGCGCAGCACCAAACACCATccc > 2:4916669/2‑76 (MQ=255) gggCGTAGAGGTGCTGGAGCAGGGGCGCGAGGCGAGGGATTCCGCCTGGAAGCGCGCAGCACCACACACCATCCCg > 1:5340750/1‑76 (MQ=255) gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg > 1:1187968/1‑76 (MQ=255) gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg > 1:1676601/1‑76 (MQ=255) gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc < 2:1070187/76‑1 (MQ=255) gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc < 1:5577654/76‑1 (MQ=255) gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc < 1:4893701/76‑1 (MQ=255) cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc < 1:1549442/76‑1 (MQ=255) cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc > 2:1427335/1‑76 (MQ=255) cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAccgc > 2:668394/1‑76 (MQ=255) cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAACATCCCGAcggc > 1:4056430/1‑76 (MQ=255) aGAAGGGCGGGAGCGGGGGAGCGATACGATGGTTTCCTCCGGGTACCGCGCCGCACTAATCACCATCCCGAcggcg < 2:255335/76‑1 (MQ=255) aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg < 1:701215/76‑1 (MQ=255) aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg < 1:758166/76‑1 (MQ=255) aaGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc < 1:3219869/76‑1 (MQ=255) aaGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc < 1:905840/76‑1 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACGAAACACCATCCCGACGGCGGCa < 2:4200960/76‑1 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 2:1249025/76‑1 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa > 1:471356/1‑76 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 2:2413464/76‑1 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 1:4105175/76‑1 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 2:5595107/76‑1 (MQ=255) cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGTAACCTCGCAGCACCAACCGCCCTCCCGACGGCGGCaaa < 1:3344091/76‑1 (MQ=255) cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa < 2:1279719/76‑1 (MQ=255) cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa < 1:4326275/76‑1 (MQ=255) cgcTGGAGCAGGGGATCCATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa > 2:4165759/1‑76 (MQ=255) gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGATCCGCGCAGCACCATACACCATCCCGACGGCGGCaaaa > 1:70334/1‑76 (MQ=255) gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGCCGGCGGCcaaa > 2:434670/1‑76 (MQ=255) gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa < 1:4557089/76‑1 (MQ=255) gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa > 2:4977293/1‑76 (MQ=255) gatGGAGCAGGGGATCGATGCGACGGTTTCCTCACGGAAACGAGCAGCACCAAACAACATCACGACGGCGGCgaaa > 1:3672151/3‑76 (MQ=255) gtGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAg < 2:3249373/75‑1 (MQ=255) cTGGGGCAGGGGATCGCTGCGAGGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAg < 2:4579805/76‑1 (MQ=255) tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc > 1:2933464/1‑76 (MQ=255) ggAGCGGGGGATCGATGCGATGCTTTCCTCCTGGAACGGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGcc < 1:2864763/76‑1 (MQ=255) gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg > 2:3503144/1‑76 (MQ=255) gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg < 2:1532813/76‑1 (MQ=255) aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCCAACGCCgg > 2:1678607/1‑76 (MQ=255) aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg > 2:5267456/1‑76 (MQ=255) aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg < 1:5667518/76‑1 (MQ=255) aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg > 1:1191576/1‑76 (MQ=255) gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCCTCCCGACGGCGGCACAAGcccga > 2:5268375/1‑73 (MQ=255) gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggg > 1:488333/1‑75 (MQ=255) aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtgg < 1:2960973/76‑1 (MQ=255) ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt < 2:1476762/76‑1 (MQ=255) ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGAGGTaa > 1:205707/1‑76 (MQ=255) ggtCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc > 1:2499857/3‑76 (MQ=255) gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc > 2:146696/1‑76 (MQ=255) aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTACCt > 2:5339766/1‑76 (MQ=255) gcgatgcgaggGTTTCCGCCGGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTa < 2:5825976/75‑1 (MQ=255) gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc > 2:2161819/1‑76 (MQ=255) gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc > 2:3447301/1‑76 (MQ=255) gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct > 1:5692688/1‑76 (MQ=255) cgatgGTTTCCTCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc < 1:1188328/76‑1 (MQ=255) cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc > 1:5554904/1‑76 (MQ=255) gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct > 1:1030149/1‑76 (MQ=255) gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct < 2:4657115/76‑1 (MQ=255) atgGTTTCCTCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt < 1:5713023/76‑1 (MQ=255) tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc > 1:1106509/1‑76 (MQ=255) tgGTGTCCTCCGGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc < 2:2411207/76‑1 (MQ=255) ggTTTCCTCCTGGAACGGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc < 2:4944518/76‑1 (MQ=255) ttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctg < 1:4921149/76‑1 (MQ=255) ttCCTCCTGCAACGGCGAAGCACCAAAGACCAGCCCAACGGCGGCAAAAGCCGGTCGCAACGACCTCTCATCCctt > 2:2685861/1‑75 (MQ=255) tcctccTGGACCCGCGCAGCACCAAACCCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc < 1:722089/76‑1 (MQ=255) tcctccTGGAACCGCGCAGCCCCCCACACCATCCCGACGGCGTCAAACGCCGGGGGTCACTACCTCTCTCCCctgc > 2:3829987/1‑76 (MQ=255) tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc > 2:3796664/1‑76 (MQ=255) tcctccTGAACCCGCGCAGCACCAACCACCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc < 1:2385450/76‑1 (MQ=255) cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct > 1:1296077/1‑76 (MQ=255) cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct > 1:3013382/1‑76 (MQ=255) cctccgGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct < 1:2631637/76‑1 (MQ=255) ccccctGGACCCACGCGCCGCCAAACCCCACCCCGACGGCGGCAAACGCCGGTGGTAACTACCTCTCTTCCctgct < 1:805057/73‑1 (MQ=255) ctcctGGACCCGCGCAGCACCAAACCCCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg < 1:232277/76‑1 (MQ=255) ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGGGGTACCTACCTCTCTTCCccgctg > 2:115976/1‑76 (MQ=255) tcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg > 1:1339387/1‑76 (MQ=255) ccgggACCCGCGCAGCACCAAACACCATCCCGCCGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggt < 2:3277353/73‑1 (MQ=255) ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc > 1:5324257/1‑55 (MQ=255) ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 1:4224142/1‑76 (MQ=255) ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 1:175388/1‑76 (MQ=255) ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 1:4460358/1‑76 (MQ=255) ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 2:4981491/1‑76 (MQ=255) tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg < 1:5094298/76‑1 (MQ=255) tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg < 1:4977294/76‑1 (MQ=255) tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg > 1:5319174/1‑76 (MQ=255) tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg > 1:2061122/1‑76 (MQ=255) ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg > 1:3326412/1‑76 (MQ=255) gacccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt < 1:1795667/73‑1 (MQ=255) gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt > 2:3973329/1‑76 (MQ=255) acccGCGCAGCACCAAACACCACCCCGCCGGCGGAAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa < 1:2710749/74‑1 (MQ=255) aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa > 1:2449257/1‑76 (MQ=255) cccGCGCAGCACCAAACCCCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg < 2:5424066/75‑1 (MQ=255) cgcgCAGCACCAAACACCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg < 2:1522638/76‑1 (MQ=255) || GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG > NC_000913/3950489‑3950626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |