| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,960,578 | 2 bp→CG | coding (1266‑1267/1485 nt) | gppA ← | guanosine pentaphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,960,578 | 0 | G | C | 100.0% | 354.3 / NA | 132 | L423V (CTC→GTC) | gppA | guanosine pentaphosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (60/72); total (60/72) | |||||||||||
| * | NC_000913 | 3,960,579 | 0 | C | G | 99.2% | 363.3 / ‑5.0 | 132 | R422R (CGG→CGC) | gppA | guanosine pentaphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (59/72); minor base A (1/0); total (60/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA > NC_000913/3960509‑3960646 || cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcgg > 1:1539593/1‑76 (MQ=255) gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggg > 1:5365395/1‑75 (MQ=255) gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc < 1:307086/76‑1 (MQ=255) gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc < 1:3783056/76‑1 (MQ=255) gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc < 1:4702144/76‑1 (MQ=255) agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg < 2:2761647/76‑1 (MQ=255) aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt > 2:3947458/1‑76 (MQ=255) aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt < 2:5000075/76‑1 (MQ=255) aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt < 1:671074/76‑1 (MQ=255) tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa > 1:513224/1‑76 (MQ=255) cGTCACGGCGACGGCTGTCAAAAATGATGTCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc < 1:5583435/76‑1 (MQ=255) gTCGCGTCGTCGGCTGGCAAAAATGCTGGCGAGGCGTAGTAAAGTGCCGAGTTGTTCTGCGACGCGCGGCGGTTCg < 2:2245476/76‑1 (MQ=255) gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACg > 2:3867731/1‑76 (MQ=255) gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACg > 2:1710550/1‑76 (MQ=255) tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg > 1:1578354/1‑76 (MQ=255) cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc > 2:805927/1‑76 (MQ=255) cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc < 1:319276/76‑1 (MQ=255) aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa < 2:3013182/76‑1 (MQ=255) cGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGcct > 2:4503362/1‑74 (MQ=255) cGGCGACGGCGGGCAAAAATGATGGCCAGGCGTAGTAAACGGCACAGGTGTTCAGAGACGCGCGGCGGCACgggct > 2:4372951/1‑73 (MQ=255) ggCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtt > 1:2800957/1‑76 (MQ=255) cGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtttt < 1:4601176/76‑1 (MQ=255) gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg > 1:3610882/1‑76 (MQ=255) gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg > 2:937449/1‑76 (MQ=255) gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg < 1:3305082/76‑1 (MQ=255) gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg > 1:2317126/1‑76 (MQ=255) aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc > 1:90022/1‑76 (MQ=255) aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc < 1:5077018/76‑1 (MQ=255) aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc < 1:4412451/76‑1 (MQ=255) ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg > 1:5649437/1‑76 (MQ=255) ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg > 1:4903114/1‑76 (MQ=255) ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg > 1:5821789/1‑76 (MQ=255) gCTGGCAAAAATGAGGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa < 2:5267732/76‑1 (MQ=255) gtGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt < 2:5036343/75‑1 (MQ=255) cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt < 2:4475729/76‑1 (MQ=255) cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt > 1:2072488/1‑76 (MQ=255) gggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATg < 1:347259/75‑1 (MQ=255) ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGc > 1:7654/1‑76 (MQ=255) gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa > 1:2330830/1‑76 (MQ=255) gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa > 2:1907435/1‑76 (MQ=255) gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa < 2:513224/76‑1 (MQ=255) aaaagTGATGTGCAGGCATAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc < 1:4372952/76‑1 (MQ=255) aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa < 2:4353805/76‑1 (MQ=255) aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa > 2:2339938/1‑76 (MQ=255) aaaTGAGGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa < 2:5748751/76‑1 (MQ=255) aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCCTTTTGCCGCTGCAGCgct > 2:588078/1‑74 (MQ=255) aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAt < 1:5466917/76‑1 (MQ=255) aaTGATGGCCAGGAGAAGAAAACGGCAGAGGTGTTGCGCGACGCGCGGCCGCCGGGGATGTTGGTGATGCAGCgct > 1:3222460/1‑74 (MQ=255) atgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATg > 2:2234423/1‑76 (MQ=255) atgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATg < 2:2152991/76‑1 (MQ=255) gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgag < 2:5063302/76‑1 (MQ=255) gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgag < 1:461051/76‑1 (MQ=255) tgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag > 1:2888159/1‑76 (MQ=255) tgGCCAGGCGTAGGAAAGGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag > 1:2594137/1‑76 (MQ=255) ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga > 1:481656/1‑76 (MQ=255) ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga < 2:880839/76‑1 (MQ=255) gcgaGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTTCGGCATTTTGCTGATGCAGCGGTGAGAGAt < 2:3718830/73‑1 (MQ=255) ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc < 1:3155168/76‑1 (MQ=255) ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc < 1:1967425/76‑1 (MQ=255) ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc < 2:1421098/76‑1 (MQ=255) cAGGCGTGGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg < 2:2929369/76‑1 (MQ=255) cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGCGAGATCg > 1:4171072/1‑76 (MQ=255) cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg < 2:3556064/76‑1 (MQ=255) cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGGACGGCATTTTGCTGATGCAGGGGTGAGAGATCg > 2:5633442/1‑76 (MQ=255) aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa > 1:563759/1‑76 (MQ=255) aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa > 1:1741278/1‑76 (MQ=255) aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa < 1:4338733/76‑1 (MQ=255) aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCCATGATAGATCGa > 1:1781389/1‑76 (MQ=255) ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc < 1:2558986/76‑1 (MQ=255) gCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc < 1:4184222/76‑1 (MQ=255) cGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCCTTTTGCTGATGCAGCGATGAGAGATCGACCg > 2:1477573/1‑76 (MQ=255) gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg < 1:1536238/76‑1 (MQ=255) gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg > 1:2679742/1‑76 (MQ=255) gtagtaAACGGCAGAGGTGTTCTGCGACGCGCGGAGGAACGGGAGTTTGCCGATGCAGCCGTGCGAGATGGATCgg > 1:3491763/1‑76 (MQ=255) tagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGa < 1:5259213/76‑1 (MQ=255) gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt > 1:1007872/1‑76 (MQ=255) gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt < 2:4227331/76‑1 (MQ=255) aaaCGGCAGAGTTGTTCTGCGGCGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAg < 2:549137/76‑1 (MQ=255) aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt < 2:1007872/76‑1 (MQ=255) aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt > 2:4717175/1‑76 (MQ=255) aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc > 1:491899/1‑76 (MQ=255) aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc < 1:3947459/76‑1 (MQ=255) aCGGCAGAGTTGTTCTGCGACGCGCCGCGGCAAGGCACCTTGCTGATGCAGCGATGGGAGGTCGATCGGGATAGTc > 1:209492/1‑76 (MQ=255) cGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCt < 2:2305579/76‑1 (MQ=255) ggCAGAGTTGTTCTGCGACACGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTg > 1:4095266/1‑76 (MQ=255) gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTgg < 2:2399727/76‑1 (MQ=255) cAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGt < 2:3491763/76‑1 (MQ=255) agagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt < 2:2631798/76‑1 (MQ=255) gagTTGTTCTGCGACGCGCGGCGTTACGGCATTTTGCTGATGCAGCGATGAGAGATCGGCCCGCTTAGTCTgggcg > 2:482071/1‑73 (MQ=255) gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCCGCGATGAGAGATCGGTCGGATTAGTCTGGTTg < 1:589075/76‑1 (MQ=255) gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGCGATCGCCCGGATTAGTCTGGTTg > 2:110424/1‑76 (MQ=255) gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg < 2:2038296/76‑1 (MQ=255) agTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTgg > 2:4755612/1‑75 (MQ=255) gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAg > 2:4313861/1‑76 (MQ=255) ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGc < 1:5106050/76‑1 (MQ=255) tgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa < 1:2241529/76‑1 (MQ=255) gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg < 1:2489041/76‑1 (MQ=255) ttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGTGCAGt > 2:3835414/1‑76 (MQ=255) tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa < 2:1848203/76‑1 (MQ=255) tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa < 1:4503363/76‑1 (MQ=255) || CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA > NC_000913/3960509‑3960646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |