| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,969,103 | 2 bp→GC | coding (232‑233/1263 nt) | wecC → | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,969,103 | 0 | C | G | 99.3% | 401.9 / NA | 148 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (79/68); minor base T (0/1); total (79/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,969,104 | 0 | G | C | 98.6% | 374.1 / NA | 143 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (73/67); minor base A (2/0); total (75/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 148 total aligned reads displayed.
CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG > NC_000913/3969035‑3969171 || tgAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct > 2:553365/2‑76 (MQ=255)ggAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct < 2:5324035/75‑1 (MQ=255) gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 2:2443621/1‑76 (MQ=255) gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:2671335/1‑76 (MQ=255) ggtgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa < 2:4329810/75‑1 (MQ=255) tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 1:2045513/76‑1 (MQ=255) tagtaAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:2794505/76‑1 (MQ=255) tagtaAAACCGGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:179493/76‑1 (MQ=255) tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:5450704/76‑1 (MQ=255) agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 1:1375061/1‑76 (MQ=255) agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 2:2001420/1‑76 (MQ=255) taAAACCTGCCGGAGAAGGCGGTGTTTTACGAGGGAGCAGGACGCCGGTTGAAGCGGATGCCTGGCTGATTGCTGt < 2:1035919/76‑1 (MQ=255) aaaacCTGCCGTAGAGGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa < 2:2157772/76‑1 (MQ=255) aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 1:3256200/1‑76 (MQ=255) aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 2:2288772/1‑76 (MQ=255) aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc > 1:5470265/1‑76 (MQ=255) ccTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa < 2:1315850/75‑1 (MQ=255) aCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa < 1:553365/76‑1 (MQ=255) cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc < 1:5451150/76‑1 (MQ=255) cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc > 2:5336222/1‑76 (MQ=255) tGCCGTGGAGGGCGTTTTTTTACGAGGGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg < 1:3470525/76‑1 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:803433/1‑76 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:4629425/1‑76 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGACGCCTGGCTGGCTGATGTAGCCACg > 1:2357829/1‑76 (MQ=255) gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc < 2:3257876/76‑1 (MQ=255) gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc < 1:476922/76‑1 (MQ=255) gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc > 1:5575484/1‑76 (MQ=255) ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc > 1:3420599/1‑76 (MQ=255) cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg > 2:4143753/1‑76 (MQ=255) gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 2:3238406/1‑76 (MQ=255) aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGctt > 2:1165153/1‑76 (MQ=255) gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa < 2:3965360/76‑1 (MQ=255) aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGGCGTTTaa > 2:338555/1‑76 (MQ=255) aGGCGGTTTTTTACGAGCGAGCACGACGCCCGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg > 2:2458665/1‑76 (MQ=255) ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg < 2:3059087/76‑1 (MQ=255) ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg < 1:108980/76‑1 (MQ=255) gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg < 2:428528/76‑1 (MQ=255) gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg > 1:4489204/1‑76 (MQ=255) gCGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCGGGCTGATTGCTGTACCCACGCCGGTGAAggg < 2:471446/76‑1 (MQ=255) gggTTTTTTACGAGCGGGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc < 2:3752341/75‑1 (MQ=255) cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc > 1:3886330/1‑76 (MQ=255) ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg > 1:3437174/1‑76 (MQ=255) ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg > 1:4641953/1‑76 (MQ=255) ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt < 1:2870789/76‑1 (MQ=255) ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt < 2:5696443/76‑1 (MQ=255) tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGCTTGCTGTACCCACGCCGTTTAATGGCGATc > 1:4977345/1‑76 (MQ=255) tttttACGAGCGAGCACGACGCCAGCTGAAGAGGATGACCGCACGATTCCCGCACCCACGCCGTCTAAGcgcgccc > 2:336295/1‑73 (MQ=255) tttgACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa < 2:568519/76‑1 (MQ=255) ttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg > 1:2828138/1‑76 (MQ=255) tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa > 2:2917693/1‑76 (MQ=255) tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa < 1:1348197/76‑1 (MQ=255) gaagagcgaggAGGGCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa < 2:5579527/73‑1 (MQ=255) aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg > 2:2709203/1‑76 (MQ=255) aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg < 1:1111474/76‑1 (MQ=255) aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg < 2:4236554/76‑1 (MQ=255) cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc < 1:5336223/76‑1 (MQ=255) gagcgagcACGACGCCAGTAGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc < 2:3351301/76‑1 (MQ=255) cgcgggcACGCCGCCATTTGAAGCGTATTGCTGGCTGCTTGCTGTCCCCACGCCTTTTACGGCCGATCATGAGCCa < 1:4739976/75‑1 (MQ=255) agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa > 2:5258890/1‑76 (MQ=255) agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa > 2:2678208/1‑76 (MQ=255) gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg < 1:4396605/76‑1 (MQ=255) gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg > 1:652473/1‑76 (MQ=255) cgagcACGACGCCAGTTGAAGCGGATGCCTGGCCGATTGCGGTACCCACGCCGTTCAAGGGCGATCACGAGCCAGa > 2:1080326/1‑76 (MQ=255) gagcAGGACGCCAGTTGAAGCGGATGCCTGGCTGATTGATGGAGCCACGCCGTTTAAGGGCGCTCATGAGCGAggt > 1:1186429/1‑74 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 1:1570429/76‑1 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 1:550377/76‑1 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:835252/1‑76 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:1847321/1‑76 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 2:540152/76‑1 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:63599/1‑76 (MQ=255) gagcACGAAGCCAGTTGAAGCGCATGCCAGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAGCATGAGGCAGat > 2:3516766/1‑76 (MQ=255) agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata < 1:2001420/76‑1 (MQ=255) gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat < 2:2562011/76‑1 (MQ=255) cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg < 1:3712417/76‑1 (MQ=255) cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGGTTAAGGGACATCATGAGCCAGATATg > 2:1190103/1‑76 (MQ=255) acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa < 1:2288772/76‑1 (MQ=255) acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa > 2:1659336/1‑76 (MQ=255) cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc > 1:4164764/1‑76 (MQ=255) gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg < 2:1312223/73‑1 (MQ=255) acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt < 1:195279/76‑1 (MQ=255) acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt < 1:1078457/76‑1 (MQ=255) cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa < 1:5776344/76‑1 (MQ=255) cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa > 1:2541797/1‑76 (MQ=255) gCCAGTTGAAGCGGATGCCTGTCTGATTGCTTTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 2:5246081/76‑1 (MQ=255) gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 1:2315924/76‑1 (MQ=255) gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc > 2:5443545/1‑76 (MQ=255) gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 1:3024900/76‑1 (MQ=255) ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg > 1:3128147/1‑76 (MQ=255) aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt > 1:213747/1‑76 (MQ=255) aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt < 2:4727195/76‑1 (MQ=255) gTTGAAGCGGATGCCTGGCTGGTTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg < 2:5736131/76‑1 (MQ=255) ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTCCGTTgc < 1:4092513/76‑2 (MQ=255) ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa < 2:4402559/76‑1 (MQ=255) tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa > 1:3809387/1‑76 (MQ=255) tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa > 1:161422/1‑76 (MQ=255) gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGCTGact > 2:583586/1‑74 (MQ=255) aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 1:3221493/1‑76 (MQ=255) aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc < 1:3378638/76‑1 (MQ=255) aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 2:2122443/1‑76 (MQ=255) aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGATCTAAGTTGAAtag > 1:1206142/1‑74 (MQ=255) || CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG > NC_000913/3969035‑3969171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |