| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,971,075 | 2 bp→GC | coding (942‑943/1068 nt) | rffG → | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,971,075 | 0 | C | G | 99.2% | 311.2 / NA | 118 | C314W (TGC→TGG) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (61/56); minor base T (0/1); total (61/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| * | NC_000913 | 3,971,076 | 0 | G | C | 99.2% | 317.3 / NA | 118 | V315L (GTG→CTG) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (60/56); minor base T (1/0); total (61/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGG > NC_000913/3971006‑3971144 || cGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGCGAACTTGGCTGGCTGccc > 2:2663038/1‑75 (MQ=255) gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc > 1:1304960/1‑76 (MQ=255) tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg > 1:5201558/1‑76 (MQ=255) tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg < 1:1950804/76‑1 (MQ=255) gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg < 1:1238415/76‑1 (MQ=255) gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg > 1:609117/1‑76 (MQ=255) gACCGTCCGGGGCATGATCTGCGTTACGCCATTGATGCTTCGAAAAGTGCCCGTGAACTTGGCCGGCTGCCGCAgg > 1:542572/1‑76 (MQ=255) cGTCCGTGGCATGATCTGCGTTCTGCCATTGACGCTTCGACAATTGCCCGTGAACTTGGCTTGCTGCCGCAGGaaa < 2:1085654/76‑1 (MQ=255) cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa < 2:4077068/76‑1 (MQ=255) cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa > 1:701346/1‑76 (MQ=255) gTCTGGGGCATGATCTGCGTTATGCCATAGCAGGTACTAACATTCCCCGCGCACTTGGCTGGCTGCCTCAGGAAAc < 1:60857/76‑1 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc > 1:4875829/1‑76 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc > 1:656963/1‑76 (MQ=255) tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc < 1:5576932/76‑1 (MQ=255) tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc > 1:5487928/1‑76 (MQ=255) ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt > 1:3703623/1‑76 (MQ=255) ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt > 1:232596/1‑76 (MQ=255) cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt < 2:5487927/76‑1 (MQ=255) cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt < 2:4580984/76‑1 (MQ=255) cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt > 2:2227520/1‑76 (MQ=255) aggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTACACATGGCAGGCTGCCGCCGGAAAccat > 2:5245918/2‑74 (MQ=255) ggggCATGATCTGCGTTATGCCATTGATGCTTCGCAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCttt > 1:5298004/1‑76 (MQ=255) ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCttt < 1:2574565/76‑1 (MQ=255) gggCGTGATCTGCGTTAGGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg < 2:1482827/76‑1 (MQ=255) gggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCGTTg > 1:1383054/1‑76 (MQ=255) ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTACCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGa > 1:3619158/1‑76 (MQ=255) tcATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa > 2:4722323/2‑76 (MQ=255) gCATGATCTGGGTTATGCCATCGCAGCTCTGACACTTGCCCTTCAACTCTGCTGGCTGCCGCAGGAAACCtttaac < 1:1280819/76‑4 (MQ=255) gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa > 1:3256026/1‑76 (MQ=255) gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa < 2:1770513/76‑1 (MQ=255) gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa < 1:5539107/76‑1 (MQ=255) cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaaa > 1:5151205/1‑76 (MQ=255) aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAg < 2:2620917/76‑1 (MQ=255) aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAg > 2:4338614/1‑76 (MQ=255) tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGt > 1:4099368/1‑76 (MQ=255) tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGt < 1:2458398/76‑1 (MQ=255) aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg < 1:2491363/76‑1 (MQ=255) tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa > 2:2947775/1‑76 (MQ=255) tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa > 1:5021223/1‑76 (MQ=255) tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa < 1:2293470/76‑1 (MQ=255) tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa > 2:475094/1‑76 (MQ=255) cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa < 2:3015651/76‑1 (MQ=255) cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa > 1:2717671/1‑76 (MQ=255) cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa < 2:5324981/76‑1 (MQ=255) tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTTAAAGTGGAAt > 1:4680630/1‑76 (MQ=255) tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGCAAGTGGAAt > 2:3134840/1‑76 (MQ=255) tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt < 1:2568741/76‑1 (MQ=255) cGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc < 1:5299793/76‑1 (MQ=255) gTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCg > 1:194178/1‑76 (MQ=255) ttATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGt < 1:1917293/76‑1 (MQ=255) ttATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGt < 1:2409426/76‑1 (MQ=255) tATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa < 2:4834238/76‑1 (MQ=255) tATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa < 1:2078597/76‑1 (MQ=255) aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa < 1:4033324/76‑1 (MQ=255) cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACg < 2:2264136/76‑1 (MQ=255) cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACg < 1:2545759/76‑1 (MQ=255) aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACgg > 1:135578/1‑76 (MQ=255) aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACgg > 1:3095951/1‑76 (MQ=255) tggATGCTGCGAAAATGGCCCGTGAACTTGGGTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt < 2:4698700/74‑1 (MQ=255) gATGCTTCGAAAATTGCCCGTGCACTTGGCTGGCTGCCGCAGGAAACCTCTGAAAGTGGAATGCGTACACCGGATc > 2:355508/1‑76 (MQ=255) gATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGACACCTTTGAAAGCGGACTGCGTAAAACgggcc > 2:344469/1‑73 (MQ=255) gATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc > 2:283369/1‑76 (MQ=255) aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa < 2:4247690/76‑1 (MQ=255) cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg < 2:4543431/76‑1 (MQ=255) cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg < 1:5493904/76‑1 (MQ=255) ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCCGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg > 1:4907971/1‑76 (MQ=255) ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg > 1:1706271/1‑76 (MQ=255) ttCGAAAATTGCCCGTGAACTTGGCTGGCTGACGCAGGAAACCTTTGAAAGTGGNATGCGTAAAACGGTTCAGTgg < 1:347662/76‑1 (MQ=255) tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt < 1:76477/76‑1 (MQ=255) gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg > 2:352101/1‑35 (MQ=25) gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAATCCTTTGAAAGTGGAATTCGTCAAACGGTTCAGTGGTAt < 2:2281978/76‑1 (MQ=255) gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAt > 2:4830268/1‑76 (MQ=255) gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACATTTGGAAGTGGGATGCGTAAAACGGCTCAGTGGTAt > 1:4953616/1‑76 (MQ=255) aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt < 2:417825/76‑1 (MQ=255) aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt < 2:135578/76‑1 (MQ=255) aagtGCCCGTGAGCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg < 2:347339/73‑1 (MQ=255) aaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg > 1:17966/1‑76 (MQ=255) tGCGCGTGTGCTTGGTTGTGTGCGGGAGGAACTGTATGACAGTGTAGTGGGTAACACGGTTCAGTGGTGTCTGGCt < 2:1470580/76‑1 (MQ=255) tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt < 2:4040339/76‑1 (MQ=255) tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt < 2:5056504/76‑1 (MQ=255) gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa > 2:727757/1‑76 (MQ=255) gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa < 2:1365943/76‑1 (MQ=255) gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa < 2:1304960/76‑1 (MQ=255) cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa > 2:107726/1‑76 (MQ=255) cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa > 1:3932500/1‑76 (MQ=255) cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTGCAGTGGTAGCTGGCTaa > 1:4291045/1‑76 (MQ=255) gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa > 1:3368769/1‑76 (MQ=255) gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa > 1:4414695/1‑76 (MQ=255) tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaa < 2:3753375/76‑1 (MQ=255) gAACTTGGCTGGCTGCCGCAGGGAACCTTTGATAGTGGAATGCGTAAAACGTTTCCAGGGTAGATGGCGAATGaaa > 1:1235855/1‑76 (MQ=255) gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa < 1:4615780/76‑1 (MQ=255) gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGGTCAGTGGTATCTGGCTAATGaaa > 1:1198305/1‑76 (MQ=255) aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAg > 1:5538114/1‑76 (MQ=255) aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAg < 2:5167468/76‑1 (MQ=255) aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc > 1:1868142/1‑76 (MQ=255) aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCCGGCTAATGAAAGc > 2:4432443/1‑76 (MQ=255) cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt > 2:3522043/1‑76 (MQ=255) ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg < 1:4393043/76‑1 (MQ=255) ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg < 2:3648980/76‑1 (MQ=255) tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg < 2:4857166/76‑1 (MQ=255) || CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGG > NC_000913/3971006‑3971144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |