| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,972,637 | 2 bp→GC | coding (430‑431/546 nt) | wecD → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,972,637 | 0 | C | G | 97.7% | 324.0 / NA | 130 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base G (64/61); total (66/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,972,638 | 0 | G | C | 99.2% | 338.1 / NA | 130 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (65/64); total (66/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 131 total aligned reads displayed.
GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC > NC_000913/3972567‑3972706 || gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTcgc > 1:5117582/1‑76 (MQ=255) gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCgg < 1:3130640/76‑1 (MQ=255) aTTGGCGTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTa < 2:256139/76‑1 (MQ=255) ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaa < 2:2977865/76‑1 (MQ=255) ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCCTATGCTCGCGGTaa < 2:4736266/76‑1 (MQ=255) tgggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaa < 1:696792/74‑1 (MQ=255) tGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaa < 1:808381/76‑1 (MQ=255) tGGCCTCCTGGCTGGACGAGGTGCAGGGGCTGAGCTGATGCAAACGGCGCTGAACTGCGTGTAGTGTCGAGGTata > 1:4454822/1‑74 (MQ=255) ggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaaa > 1:3040394/1‑76 (MQ=255) gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaac < 2:3532822/76‑1 (MQ=255) ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaaca > 1:4518316/1‑76 (MQ=255) ctgctgGCTGGACGCGGTGCAGGTGCTGCGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaa < 2:4829553/76‑1 (MQ=255) ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAcacaa > 1:5731044/1‑76 (MQ=255) tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaac > 1:4567501/1‑76 (MQ=255) gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACt < 2:2537708/76‑1 (MQ=255) ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGGAAAACAACtt > 2:4912518/1‑76 (MQ=255) tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACttt < 2:4742466/76‑1 (MQ=255) tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACttt < 1:5362438/76‑1 (MQ=255) gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGc < 1:4597212/76‑1 (MQ=255) gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGc < 1:2389794/76‑1 (MQ=255) ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCg < 1:883784/76‑1 (MQ=255) gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAGCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt < 2:4873534/76‑1 (MQ=255) gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt < 1:5469788/76‑1 (MQ=255) gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt < 1:1143251/76‑1 (MQ=255) gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt > 1:3216990/1‑76 (MQ=255) gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg < 2:3767620/75‑1 (MQ=255) gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg < 2:1211543/75‑1 (MQ=255) aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg > 2:5525582/1‑76 (MQ=255) aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg > 2:3486567/1‑76 (MQ=255) aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg < 2:1381414/76‑1 (MQ=255) cgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTgg < 2:78926/76‑1 (MQ=255) cgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTgg < 2:2805616/76‑1 (MQ=255) gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGc > 2:4259920/1‑76 (MQ=255) gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGc > 1:1145746/1‑76 (MQ=255) ggTGGAGGGGCGGAGCTGGTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa < 2:233962/76‑1 (MQ=255) ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAACACAACTTTGCGGGGGGCgc > 2:5819699/1‑75 (MQ=255) ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa > 2:2837023/1‑76 (MQ=255) ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa > 1:3201533/1‑76 (MQ=255) ggTGCAGGGGCGGAGCTGAGGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa < 2:5443874/76‑1 (MQ=255) gTGCAGGTGCTGCGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAc < 1:5127167/76‑1 (MQ=255) tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAcc > 2:1858779/1‑76 (MQ=255) tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGCGGAGAcc > 1:2300007/1‑76 (MQ=255) tGCAGGTGCGGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAca < 2:4889208/76‑2 (MQ=255) gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGGGGCGAccc > 2:142773/1‑76 (MQ=255) cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCa > 2:2385138/1‑76 (MQ=255) aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAGCTTTGCGGGTGGCGTCCCaa < 2:3420676/76‑1 (MQ=255) aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 2:2277872/1‑76 (MQ=255) aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 1:4730019/1‑76 (MQ=255) aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 2:4257131/1‑76 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaca > 1:2618272/1‑74 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa > 1:2792163/1‑76 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa > 2:4435956/1‑76 (MQ=255) gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt < 1:5648692/76‑1 (MQ=255) gctgagctgaTGCAAACGGCGCTAAACTGGGCGCATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAACgg > 1:4022595/1‑76 (MQ=255) tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc < 1:1017148/76‑1 (MQ=255) gagctgaTGCAAACGTCGCTAAACGAGTCTTATGCTCGCGGTAAACCAACGTTGCGGGTTGCGACCCAACTGTGCa < 1:2664159/76‑1 (MQ=255) gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa < 2:927955/76‑1 (MQ=255) ggctaaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa < 2:5731042/75‑1 (MQ=255) gctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAac < 1:5002378/76‑1 (MQ=255) ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca > 2:3874461/1‑76 (MQ=255) ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca > 1:5736975/1‑76 (MQ=255) gtGCAGACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg < 2:215507/75‑1 (MQ=255) aTGCAAACGGCGCTAACCTGGGCTTATTCTCGCGTTAAAACAACTTTGCGGGGGTAGACCACAATGTGCACGAccg > 2:2727275/1‑76 (MQ=255) aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg > 1:608573/1‑76 (MQ=255) aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg > 2:4927557/1‑76 (MQ=255) aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAACGGGCAACAccg > 2:2699638/1‑76 (MQ=255) gCAACCGGCGCGAGACTGGGCGTATGCTCGCGGTAAAACCGCTTTGCGGGTGGCGGCCCAAATGGGCAACAgcgcc < 1:267100/76‑1 (MQ=255) gCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 1:5525584/76‑1 (MQ=255) gCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:2956394/76‑1 (MQ=255) aaaaCGGCGCGAAGCGGGGCGTAGGCTCGCGGTAAAACACCTTGGGGGGTGGCGCCCAAAAGGGGCAACAccgccg < 2:5117581/75‑1 (MQ=255) aaaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc > 1:3780863/1‑76 (MQ=255) aggggCGCTAAGGGGGGCGTATGCTGGGGGTAAAACAACTTGGGGGGGGGCGACCCAAAGGGGCAACACCGCcgcg < 2:4287551/73‑1 (MQ=255) accGGCGCAAGACTGGGCGGAGGCTGGCGGTAAAACAATTTGGCGGGTGGCGCCCAAAAGGGGCAACACCGCcgcg < 2:3123634/74‑1 (MQ=255) aaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg > 2:3985863/1‑76 (MQ=255) aCGGCGCTAAACTGGGCGTATGCTAGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc > 2:3326321/1‑76 (MQ=255) cGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt > 2:823452/1‑76 (MQ=255) cGGCGCTAAACGGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt < 1:1691591/76‑1 (MQ=255) ggCGCTAACGTGGGCGTATGCTCGCGGTAAAACAACGTTGCGGTGGGCGACCCAAATGGGCAACACCGCCGCGCtt < 1:3626562/76‑1 (MQ=255) ggCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt < 1:4883464/76‑1 (MQ=255) ggCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt > 1:1734646/1‑76 (MQ=255) ggCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt > 2:5304131/1‑76 (MQ=255) gccgcAAAACGGGGCGTATCCCCGCGGAAAAACACCTTGGCGGGGGGCGACCCAAAGGGGCAACACCGCCGCGCtt < 1:1989849/74‑1 (MQ=255) gcgcTAAACTGGGCGTATGCTCGCGGTACAACAACTTTGCGGGTGGATACCCAAATTTGCAACACCGCCGAGCCTa > 1:1485666/1‑76 (MQ=255) gcgcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa > 1:1441239/1‑76 (MQ=255) gcgcGAAACGGGGGGTAGCCGCGCGGTAAAACAACTTTGCGGGTGGCGACCAAAAGGGGCAACACCGCCGCGCTTa < 2:1145746/76‑1 (MQ=255) ggcTAAACGGGGCGTAGGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa < 2:3190285/75‑1 (MQ=255) ggcTAAACGGGGCGTAGGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa < 2:3159511/75‑1 (MQ=255) cgcTAACCGGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGCCCCAAATGGGCAACACCGCCGCGCTTaa < 1:3983023/76‑1 (MQ=255) gcTAAACTGGGCGTATGCTTGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa > 1:4072583/1‑76 (MQ=255) cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 2:1209590/1‑76 (MQ=255) tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAcn < 1:3591325/76‑2 (MQ=255) tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACg > 1:3062561/1‑76 (MQ=255) tAAACGGGGCGTAGCCTCGGGGAAAACCAACTTGGGGGGTGGCGACCAAAGTGGGCAACCCCGCCGCGCTTAAACg < 2:1610880/76‑1 (MQ=255) aaaCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa > 1:1855574/1‑76 (MQ=255) aCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata > 1:1750037/1‑76 (MQ=255) cTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac > 1:354706/1‑76 (MQ=255) gggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat > 1:4078167/1‑76 (MQ=255) gggCGTAGGGTGGCGGTAAAACAACTTTGCGGGGGGCGACCAAAAGGGGAAACACCGCCGCGCTTAAACGatacat < 2:5404472/76‑1 (MQ=255) ggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata > 2:4839664/1‑76 (MQ=255) gCGTATGCTCGCGGAAAAACAACTTGGCGGGTGGCGCCCAAAATGGGCAACACCGCCGCGCTTAAACGatacatac < 1:2450955/76‑1 (MQ=255) || GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC > NC_000913/3972567‑3972706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |