| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,001,421 | 2 bp→GC | coding (381‑382/903 nt) | rarD ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,001,421 | 0 | C | G | 98.2% | 308.2 / ‑4.5 | 113 | G128R (GGA→CGA) | rarD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base G (48/63); total (50/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,001,422 | 0 | G | C | 99.1% | 309.3 / ‑4.6 | 113 | D127E (GAC→GAG) | rarD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (49/63); total (50/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑CGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCG > NC_000913/4001353‑4001487 || aGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAAtc‑gctcgcag > 2:1125437/1‑74 (MQ=255)aGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCg > 1:5521801/1‑76 (MQ=255) ccccACAGCGGGACTACCCCGCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGa < 1:2472397/74‑1 (MQ=255) tCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAg > 2:4641265/1‑76 (MQ=255) ccACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAgg < 1:1234362/76‑1 (MQ=255) ccACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAgg > 1:4161841/1‑76 (MQ=255) ccACAGCTGAACAAACACCCCACATACCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAgg < 2:793644/76‑1 (MQ=255) cacaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGa > 1:2657242/1‑76 (MQ=255) cacaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGa > 2:4267189/1‑76 (MQ=255) cacaGCGGGACTACCACGCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGa < 1:2467994/76‑1 (MQ=255) ccaGCTGGACAAACCCCCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGaa < 1:5317266/75‑1 (MQ=255) acaGCTGGACTAACACGCCACATATCGCCAGAACCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGaa > 2:3519567/1‑76 (MQ=255) cggCTGGACTAACACG‑CACATACCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGaaaa < 2:222186/74‑1 (MQ=255) aGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGaaaa > 2:4538035/1‑76 (MQ=255) gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAat > 1:5513778/1‑76 (MQ=255) cTGGACTAACACGCCACATATCGCCAGAAGCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAatc > 2:3423180/1‑76 (MQ=255) gcaCTACCCCGCCACATATCCCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAatcat < 1:2736534/74‑1 (MQ=255) taCTAACCCGCCACAGAACGCGAGAACCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAatcatc < 2:4129919/75‑1 (MQ=255) aCTAACCCCCCACATATCGCCGCATTCTCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCTCCGAGGACAATCATcc < 1:3832350/76‑1 (MQ=255) cTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATccc > 1:3646485/1‑76 (MQ=255) tAACACGCCATATATCGCCAGAATCACCGCCAGCCATTGCATACGGCGGAATC‑GCTCGCCGACGAAAATCATcccc > 1:1153113/1‑76 (MQ=255) tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATcccc > 1:810870/1‑76 (MQ=255) aaaCACCCCACATATCCCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATcccc < 2:347529/75‑1 (MQ=255) acGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGca > 2:4432094/1‑76 (MQ=255) ccccACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGcac < 2:3694183/74‑1 (MQ=255) gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGcaca > 1:3848543/1‑76 (MQ=255) ccACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACaa > 1:1659406/1‑76 (MQ=255) cacaTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAAt > 1:2723937/1‑76 (MQ=255) cacaGATCGCAAGAATCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAAt < 2:3942079/76‑1 (MQ=255) atatatCGCTAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATg < 1:2728149/74‑1 (MQ=255) acaTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATg > 2:3852857/1‑76 (MQ=255) acaTACCGCAAGATTCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATg < 1:4290613/76‑1 (MQ=255) caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGt < 2:4161840/76‑1 (MQ=255) caTATCGCCAGAATCACCACCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGt < 2:4054257/76‑1 (MQ=255) caTATCGCCAAGACCCCCGCCAGCCATTGCATCCTGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGt < 1:3921415/76‑1 (MQ=255) caTAGCTCCAGAACCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGt < 2:3513654/76‑1 (MQ=255) tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc < 1:1562227/76‑1 (MQ=255) tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc > 1:5547754/1‑76 (MQ=255) tatCGCAAGAATAACCGACAGCCAGTGCATCCGGCGGAATCGCG‑CGCAGAGGAAAATCATCCCCAGCACAATggac > 1:2351581/1‑73 (MQ=255) atCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa > 2:2144066/1‑76 (MQ=255) atCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa > 2:2916615/1‑76 (MQ=255) cGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAcc < 1:4531187/76‑1 (MQ=255) ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg > 2:4290477/1‑76 (MQ=255) ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg > 1:758935/1‑76 (MQ=255) cAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGtt > 2:491497/1‑69 (MQ=255) cAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGtt < 1:491497/69‑1 (MQ=255) cAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc > 2:2626226/1‑76 (MQ=255) cAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc < 1:1195027/76‑1 (MQ=255) cAGAACCCCCGCCAGCCATTGCATCCGGCGGGATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc < 2:2445111/76‑1 (MQ=255) cAGAACCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc < 2:2948720/76‑1 (MQ=255) cAGAACCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc < 1:1963660/76‑1 (MQ=255) aGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCg > 2:1939493/1‑76 (MQ=255) aGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCg > 1:2989938/1‑76 (MQ=255) aGAACCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCg < 1:4459784/76‑1 (MQ=255) aaccACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCggg < 1:3476187/73‑1 (MQ=255) aaTCCCCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCggg < 2:3718935/76‑1 (MQ=255) aaTCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCggg > 1:1084431/1‑76 (MQ=255) aaTCACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCggg > 1:4591997/1‑76 (MQ=255) aTCACCGGCAGCCATTGCAGCCGGCGGAAGC‑CCTCGCCGAGGAAAGTCAGACCCATCCCAATGTACACCAGCcggc > 2:4581146/1‑75 (MQ=255) ccACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGtt < 1:5751588/75‑1 (MQ=255) cgccGCCAGCGATTGCGTCCTGGTGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGTCAGCGGGTta < 2:3118687/74‑1 (MQ=255) cACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta < 2:2024763/76‑1 (MQ=255) cACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta < 1:2704920/76‑1 (MQ=255) cACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta > 2:1622259/1‑76 (MQ=255) cACCGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta < 1:3293485/76‑1 (MQ=255) cccGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaa < 1:331392/75‑1 (MQ=255) aCCGCCAGACATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaa < 2:1832977/76‑1 (MQ=255) ccGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaat > 1:1357452/1‑76 (MQ=255) cGCCAGCCATTGCATCCGGCGGAATC‑GCTCGCCCAGGAAAATCATCCCCAACACACTGACCACCAGCGGCCtaata > 2:5850548/1‑76 (MQ=255) gccagccaTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaataa < 2:2657242/76‑1 (MQ=255) cagccaTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg > 2:3916838/1‑76 (MQ=255) cagccaTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg > 2:4342367/1‑76 (MQ=255) agccaTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAga > 1:1090264/1‑75 (MQ=255) ccaTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTaa < 1:1875390/76‑1 (MQ=255) ccaTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTaa < 1:1900358/76‑1 (MQ=255) caTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAc < 2:5417732/76‑1 (MQ=255) caTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAc > 1:3422427/1‑76 (MQ=255) caTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAc > 1:4806352/1‑76 (MQ=255) caTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAc < 1:911035/76‑1 (MQ=255) aTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAcc > 1:4728723/1‑76 (MQ=255) aTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAcc > 1:259018/1‑76 (MQ=255) aTTGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAcc < 2:3646485/76‑1 (MQ=255) tttcATCCGGCGGAAGC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCa < 2:2501979/73‑1 (MQ=255) tGCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCaa < 1:5604919/76‑1 (MQ=255) gCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg < 1:5671972/76‑1 (MQ=255) gCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg < 1:4865043/76‑1 (MQ=255) gCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg < 2:2812660/76‑1 (MQ=255) gCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg > 1:4185720/1‑76 (MQ=255) gCATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg < 1:2076264/76‑1 (MQ=255) cATCTGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAgg < 1:4061072/76‑1 (MQ=255) cATCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAgg < 2:3320602/76‑1 (MQ=255) tCCGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCt > 2:2813677/1‑76 (MQ=255) ccGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 2:2448828/76‑1 (MQ=255) ccGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 2:5421021/76‑1 (MQ=255) ccGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 2:1352742/76‑1 (MQ=255) ccGGCGGAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 1:682893/76‑1 (MQ=255) cggcggAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcg > 1:3880702/1‑75 (MQ=255) cggcggAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcg < 2:5547752/76‑2 (MQ=255) ggcggAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgc < 1:2559980/76‑3 (MQ=255) ggcggAATC‑GCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgc < 2:4438428/76‑3 (MQ=255) || AGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATC‑CGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCG > NC_000913/4001353‑4001487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |