| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,007,718 | 2 bp→CG | coding (943‑944/1023 nt) | pldB → | lysophospholipase L(2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,007,718 | 0 | G | C | 100.0% | 267.4 / NA | 96 | A315R (GCC→CGC) | pldB | lysophospholipase L(2) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (50/46); total (50/46) | |||||||||||
| * | NC_000913 | 4,007,719 | 0 | C | G | 99.0% | 259.1 / NA | 96 | A315R (GCC→CGC) | pldB | lysophospholipase L(2) |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (50/45); total (50/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGT > NC_000913/4007651‑4007794 || tgAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc > 2:3919248/2‑76 (MQ=255)cggaggaggTCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc < 2:5197996/73‑1 (MQ=255)cGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc < 1:661491/76‑1 (MQ=255)cGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc > 1:4392383/1‑76 (MQ=255) gAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTcg > 1:301898/1‑76 (MQ=255) aggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTc > 1:5410929/1‑76 (MQ=255) aggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTc > 1:371392/1‑76 (MQ=255) ggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTcc > 1:1257774/1‑76 (MQ=255) gcGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGACAAGGACGCAATGCGCTCAGTCGCGCTCCAc < 2:1680576/75‑1 (MQ=255) aCGGTCTTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAc < 1:1794393/76‑1 (MQ=255) aCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAc < 1:10018/76‑1 (MQ=255) aCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAc < 1:2264881/76‑1 (MQ=255) cGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTccccc > 2:703273/1‑73 (MQ=255) cGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACg < 2:1719764/76‑1 (MQ=255) ggCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGc > 2:596363/1‑76 (MQ=255) ggCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGc > 1:2260655/1‑76 (MQ=255) ggCCGTTGGGAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGGCGCGCTCCACGc > 2:648903/1‑76 (MQ=255) gCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCTCGcc < 1:378024/76‑1 (MQ=255) gCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGcc < 1:5481396/76‑1 (MQ=255) gCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGcc > 2:959315/1‑76 (MQ=255) gCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGcc > 2:2600940/1‑76 (MQ=255) ccGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCa > 2:1190628/1‑76 (MQ=255) cGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCAt < 2:2424693/76‑1 (MQ=255) cGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCAt < 2:5521197/76‑1 (MQ=255) gTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATc > 2:89918/1‑76 (MQ=255) ttGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCg > 2:4645499/1‑76 (MQ=255) tGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTGCACGCCATCGt > 1:1281978/1‑76 (MQ=255) tGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGt < 2:1137453/76‑1 (MQ=255) ggTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGtt > 2:1172553/1‑76 (MQ=255) ggTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGtt > 2:5108347/1‑76 (MQ=255) ggTAATTAAAGGTGATTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGtt < 1:658318/76‑1 (MQ=255) tAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc < 2:738922/56‑1 (MQ=255) tAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc > 1:738922/1‑56 (MQ=255) tAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGa > 1:5774644/1‑76 (MQ=255) aaTTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAt < 1:3163652/76‑1 (MQ=255) ttAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttt < 2:539075/76‑1 (MQ=255) ttAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttt > 1:668365/1‑76 (MQ=255) tAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGCtttt > 2:4691495/1‑76 (MQ=255) aaaGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttttt > 1:2553962/1‑76 (MQ=255) aaaGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttttt < 2:1106909/76‑1 (MQ=255) aaaGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttttt < 1:328551/76‑1 (MQ=255) aaaGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttttt > 2:3462193/1‑76 (MQ=255) aaGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAtttttt > 2:3904893/1‑76 (MQ=255) aaGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAtttttt < 2:5389705/76‑1 (MQ=255) tGCTCACCATGAGATACTTTTTGAAAAGGACGCAATGCGCTCAGGCGCGCTCCACGCCCTCGCTGATTTTTTCAAc > 2:3879512/1‑76 (MQ=255) gCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACa > 2:1672332/1‑76 (MQ=255) cTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAg > 2:2257741/1‑76 (MQ=255) tACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGc > 1:5257996/1‑76 (MQ=255) tACCAGGAGATGCTTTTTGAAAAGGACGCAATGCCGTCAGTCGCGATCCAGGCCATCGTTTATTTTTTCAACGGGc < 2:5508053/76‑1 (MQ=255) aCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCa > 2:2541457/1‑76 (MQ=255) ccATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCTCTCCACGCCATCGTTGATTTTTTCAACAGGCAt < 1:1146936/76‑1 (MQ=255) ccATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGCTTTTTTCAACAGGCAt > 2:1013165/1‑76 (MQ=255) ccATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGCTTTTTTCAACACGCAt > 2:77726/1‑76 (MQ=255) ccATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAt > 1:3587812/1‑76 (MQ=255) ccATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAt > 1:1531715/1‑76 (MQ=255) cATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGCTTTTTTCAACACGCaaa > 2:5616634/1‑74 (MQ=255) cATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATa > 2:5613864/1‑76 (MQ=255) aaTGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGACGCGCTCCACGCCATCGTTGCTTTTGGCCACAGGCATa > 2:784037/2‑76 (MQ=255) aTGAGATTCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATaa > 1:3105532/1‑76 (MQ=255) aTGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATaa < 2:1653888/76‑1 (MQ=255) aTGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATaa < 1:5781882/76‑1 (MQ=255) aTGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATaa > 1:4491959/1‑76 (MQ=255) tGAGCTCCTTTTTGGAAGTGACGCCATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAc < 2:5792168/76‑1 (MQ=255) tGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCGACAGGCATAAc < 1:5578277/76‑1 (MQ=255) agaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTc < 2:668365/76‑1 (MQ=255) gaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTGCACGCCATCGTTGATTTTTTCAACAGGCATAACTCa < 2:1281978/76‑1 (MQ=255) aTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAc > 1:964578/1‑76 (MQ=255) ccTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAccc < 2:5410928/76‑1 (MQ=255) ccTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAccc < 1:3030054/76‑1 (MQ=255) ccTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAccc < 2:505254/76‑1 (MQ=255) cTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCa > 2:3612559/1‑76 (MQ=255) cTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCa < 1:2291532/76‑1 (MQ=255) cTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCa < 1:3919249/76‑1 (MQ=255) cTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCa > 1:642227/1‑76 (MQ=255) cTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCa < 1:2584588/76‑1 (MQ=255) ttttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGc < 1:4645500/76‑1 (MQ=255) ttttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGc < 1:5222815/76‑1 (MQ=255) tggAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCgg < 1:821965/74‑1 (MQ=255) tGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGa < 2:5356669/76‑1 (MQ=255) gAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaa < 2:4918889/76‑1 (MQ=255) gAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaa < 1:1341296/76‑1 (MQ=255) aaaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaaa > 2:5025725/1‑76 (MQ=255) aaaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaaa > 1:4725291/1‑76 (MQ=255) aaaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaaa < 2:4392382/76‑1 (MQ=255) aaaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaaa < 1:690427/76‑1 (MQ=255) aaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATATGTCACCCAGCGGAAAc < 2:3830582/76‑1 (MQ=255) aaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAc > 1:4540477/1‑76 (MQ=255) aaaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAc > 2:3245886/1‑76 (MQ=255) aaGGACGCAATGCGCTGAGTCGCGCTCCACGCCGTCGTTGATTTTTTCACGAGGCATATGTCGCCCAGCGGaaccc < 2:4371146/76‑4 (MQ=255) aaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAcc > 1:4779935/1‑76 (MQ=255) aaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAca < 2:932229/76‑2 (MQ=255) aGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCg < 1:2541457/76‑1 (MQ=255) gACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGct > 2:4377811/1‑76 (MQ=255) aCGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGctc > 2:2985346/1‑76 (MQ=255) aCGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGctc < 1:3338912/76‑1 (MQ=255) cGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGctct > 1:5553057/1‑76 (MQ=255) ccTCAGTCGCGCTCCCCGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGt < 1:82120/76‑1 (MQ=255) || CGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGT > NC_000913/4007651‑4007794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |