| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,026,252 | 2 bp→GC | coding (110‑111/1164 nt) | fadA ← | thiolase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,026,252 | 0 | C | G | 99.2% | 331.7 / ‑6.0 | 126 | T37S (ACG→AGC) | fadA | thiolase I |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (59/66); total (60/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
| * | NC_000913 | 4,026,253 | 0 | G | C | 99.2% | 326.7 / NA | 129 | T37S (ACG→AGC) | fadA | thiolase I |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (58/69); minor base A (0/1); total (59/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 130 total aligned reads displayed.
GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA > NC_000913/4026181‑4026322 || gTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCCGCGCCGGGGTACGCCCCCGCAGGGtgc > 2:731168/1‑74 (MQ=255) ctgctgCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGc > 1:3130748/1‑76 (MQ=255) tgctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCa < 1:5369958/76‑1 (MQ=255) ggtgcCCACAACCCCAGTAAATGTCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCAt < 2:4966665/74‑1 (MQ=255) gctgcACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCAt < 1:1323156/76‑1 (MQ=255) gctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTGCGCAt > 2:4656917/1‑76 (MQ=255) ctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCAtt < 1:2039934/76‑1 (MQ=255) cggcACACACCCCCAGGAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCAtt < 2:2640678/74‑1 (MQ=255) ccccacaCCCCCAGTAAATACCGTCGGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaa < 2:653041/73‑1 (MQ=255) cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCCGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa > 2:1847275/1‑76 (MQ=255) ccacaCCCCCAGAAAATATCGGCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAt < 2:5714109/75‑1 (MQ=255) acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCCGCAGGCTACGCATTAAAt > 2:2523242/1‑76 (MQ=255) acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAt > 1:2797388/1‑76 (MQ=255) cacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATg > 1:3476249/1‑76 (MQ=255) caACCCCAGTAAATATCGTCGAGGGCCGCCGCTGCCAGCGCCGGGGTACGCGCCAGCAGGCCACGCATTCAATGac > 2:5687047/1‑75 (MQ=255) accccgAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTACATGAGc < 2:4462284/74‑1 (MQ=255) acccccAGTAAATATCGTCGGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGc < 1:1384545/74‑1 (MQ=255) aCCCCAGTAAATACCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg < 1:4807949/76‑1 (MQ=255) ccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCgg < 2:2542718/76‑1 (MQ=255) cccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGga < 2:5176650/76‑1 (MQ=255) ccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag > 1:411637/1‑76 (MQ=255) cAGTAACTATCGGCTGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgaga < 2:4438167/76‑1 (MQ=255) cAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgaga < 1:4853526/76‑1 (MQ=255) cAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCATTAAATGAGCGgaga > 1:3827009/1‑76 (MQ=255) cAGTAAATACCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgaga < 2:3504358/76‑1 (MQ=255) aGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagag > 1:740792/1‑76 (MQ=255) gaaaaTAGCGTCGGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagaga < 2:3524671/74‑1 (MQ=255) gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagaga > 2:4649198/1‑76 (MQ=255) tcaaTATCGTCGGGGGCCGCCTCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGatat < 2:4045803/74‑1 (MQ=255) tAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAt < 1:2041969/76‑1 (MQ=255) aaaTATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtc < 1:5169541/76‑1 (MQ=255) aaaTATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtc < 1:5578216/76‑1 (MQ=255) aaaTATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtc > 2:2269274/1‑76 (MQ=255) atgtcggcgGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtctt < 2:253045/73‑1 (MQ=255) atatCGTGGAGGGCCGCCGCTTCCCGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtctt < 2:542494/76‑1 (MQ=255) atatCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtctt > 1:1257104/1‑76 (MQ=255) tatCGCCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttc < 1:3257192/76‑1 (MQ=255) atCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttct > 1:5363047/1‑76 (MQ=255) tcgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg < 1:821841/76‑1 (MQ=255) cggcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgc < 2:4032123/73‑1 (MQ=255) gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCCGCAGGCTACGCGTTAAATGAGCGGAGAGCTCCTCCgcc > 2:385122/1‑75 (MQ=255) gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgca > 1:4822639/1‑76 (MQ=255) tcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGCTCTTCTgcac > 2:5411519/1‑76 (MQ=255) cgggggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg < 2:345791/73‑1 (MQ=255) cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg < 2:4248331/76‑1 (MQ=255) cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg < 1:1231289/76‑1 (MQ=255) gggggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc < 2:4107531/74‑1 (MQ=255) gacggCCGCCGCTTCCATCGCCGGGTTACGCGCCAGCAGGCTACGCATCAACCGAGCGGAGCGCACCTCTgtacgc > 2:1039282/4‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc < 2:2931493/76‑1 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 2:743084/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 1:2272263/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCAGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 1:1911156/1‑76 (MQ=255) gccgccGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGt < 2:219889/76‑1 (MQ=255) gccgccGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGt > 1:1547983/1‑76 (MQ=255) gccgccGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGt < 1:3682618/76‑1 (MQ=255) ccgccgCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt < 2:2880332/76‑1 (MQ=255) gccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc < 2:1346186/76‑1 (MQ=255) ccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg > 1:4221906/1‑76 (MQ=255) ccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg < 2:1883438/76‑1 (MQ=255) ccgcTGCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg < 2:5701819/76‑1 (MQ=255) cgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGa > 2:1013545/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAGATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa > 1:2158426/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa > 1:447073/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa > 1:4540753/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGCTACGaa > 1:1341801/1‑76 (MQ=255) gcTTCCAGAGCCGGGTTACGCGAAAGCAGGCGACGCATTAAATGAGCGGAGAGATCTGCTCCACGCACGTCACGaa > 2:5390035/1‑76 (MQ=255) ttCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaa > 1:272425/1‑76 (MQ=255) ccAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGc < 2:4117178/76‑1 (MQ=255) ccAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGc < 2:3060861/76‑1 (MQ=255) aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc < 2:1745474/76‑1 (MQ=255) gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc > 2:4940102/1‑76 (MQ=255) gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc < 1:180413/76‑1 (MQ=255) gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc < 1:4592726/76‑1 (MQ=255) gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc > 1:3325880/1‑76 (MQ=255) gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc > 1:3785358/1‑76 (MQ=255) cgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCg < 1:940522/76‑1 (MQ=255) cgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCg < 2:5410116/76‑1 (MQ=255) gggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt > 1:3433401/1‑76 (MQ=255) gggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt > 2:2889005/1‑76 (MQ=255) gggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTGCTGCACGCACGTTAAGAAAAGCAACGCCCt > 1:5751933/1‑76 (MQ=255) gggTTACGCGCCAGCAGGCTACGCATTAAATGAGAGGAGAGATCTGCTGCACGCACGTTACGAAAAGCACCGCCCt > 2:5173733/1‑76 (MQ=255) gggTTACGCGCCAGCAGGCTAAGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTGACGAAAAGCACCGCCCt > 2:4015339/1‑76 (MQ=255) gTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc < 1:1846121/76‑1 (MQ=255) gTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc > 2:2983201/1‑76 (MQ=255) ttACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCGCCGCCCTTCg > 2:2930715/1‑76 (MQ=255) ttACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg > 1:3232397/1‑76 (MQ=255) ttACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg < 2:4448612/76‑1 (MQ=255) tACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGCAAAGCACCGCCCTTCGa > 1:4322196/1‑76 (MQ=255) tACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGa < 1:1828883/76‑1 (MQ=255) aCGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGaa > 2:4771006/1‑76 (MQ=255) aCGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGATCTTCGaa > 1:2931724/1‑76 (MQ=255) aCGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATATTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGaa > 1:1520295/1‑76 (MQ=255) gcgcCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACg < 2:5034228/76‑1 (MQ=255) gcgcCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACATTACGAAAAGCACCGCCCTTCGAACg < 2:5230571/76‑1 (MQ=255) cgcCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTCCGACCga > 2:3362028/1‑75 (MQ=255) ccAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGcc < 1:1984462/76‑1 (MQ=255) agcagtATACGCGTGAAATGAGCGGAGAAGTCTTCTGCACGCACGTTACGAAAAGCGCCGCCCGTCGAACGGCCCa < 1:296215/76‑1 (MQ=255) agcaggCTACGCATTAAATGAGCTGAGCGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa < 1:42564/76‑1 (MQ=255) agcaggCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa < 1:279145/76‑1 (MQ=255) agcaggCTACGCATGAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa < 2:4737081/76‑1 (MQ=255) || GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA > NC_000913/4026181‑4026322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |