| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,194,577 | 2 bp→GC | coding (97‑98/774 nt) | yjaD → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,194,577 | 0 | C | G | 99.2% | 364.9 / NA | 133 | R33A (CGG→GCG) | yjaD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (68/64); total (69/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,194,578 | 0 | G | C | 99.2% | 339.5 / ‑4.9 | 131 | R33A (CGG→GCG) | yjaD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (67/63); minor base A (0/1); total (67/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAACGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG > NC_000913/4194505‑4194645 || gATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTCGCCGGCGGGTGAAATGCCACATGGCGAACcgg > 2:3667092/1‑76 (MQ=255) aCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa > 1:3617223/1‑76 (MQ=255) aCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa > 1:5294342/1‑76 (MQ=255) aCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa > 1:5171685/1‑76 (MQ=255) aCGGCTGGTGGGGCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGGGGCaaa > 1:912322/1‑76 (MQ=255) ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt > 1:3663646/1‑76 (MQ=255) ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt > 2:3048480/1‑76 (MQ=255) gCTGGGGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAttt < 1:2051209/76‑1 (MQ=255) cTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTc < 2:4517282/76‑1 (MQ=255) cTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGAGGCAAATTTc < 1:5409648/76‑1 (MQ=255) tggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg > 1:1103242/1‑76 (MQ=255) ggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGa < 1:1523969/76‑1 (MQ=255) ggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGa > 2:2554474/1‑76 (MQ=255) ggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGa > 2:4620677/1‑76 (MQ=255) gtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGAt > 1:3433925/1‑76 (MQ=255) ggggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGTCGAAGCGGCAAATTTCTATc < 2:4916699/75‑1 (MQ=255) ggggTCGCCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAAGTGCCATATGGCGTAGCGGCAAAGTTCGATc < 2:4909684/75‑1 (MQ=255) ggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCtt > 1:378065/1‑76 (MQ=255) ggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCtt < 2:4759026/76‑1 (MQ=255) gtcgtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAATTCGATCTtg > 2:5106911/1‑76 (MQ=255) tcgtcgGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgt < 2:3762541/76‑1 (MQ=255) cgtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgtg < 2:1589822/76‑1 (MQ=255) cgtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATATtgtg > 1:4806036/1‑76 (MQ=255) gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTgg < 2:3617223/76‑1 (MQ=255) gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTgg > 1:5744129/1‑76 (MQ=255) gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATGTCGATCTTGTgg > 1:733683/1‑76 (MQ=255) tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTggg < 1:576991/76‑1 (MQ=255) tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTggg > 1:5490069/1‑76 (MQ=255) cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa < 2:1359274/60‑1 (MQ=255) cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa > 1:1359274/1‑60 (MQ=255) cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGt > 1:233437/1‑76 (MQ=255) cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGt < 1:1564999/76‑1 (MQ=255) aGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTc > 2:4422902/1‑76 (MQ=255) aGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTc > 1:2108862/1‑76 (MQ=255) aGCCATGAACAAAAATGATGGATGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTc < 1:2800169/76‑1 (MQ=255) ccATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGAAAATTTCGATCTTGTGGGTCAg > 2:3725268/1‑76 (MQ=255) cATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgc < 2:1853327/76‑1 (MQ=255) cATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgc < 1:4027514/76‑1 (MQ=255) cATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgc > 2:3582480/1‑76 (MQ=255) aTGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcg > 2:2065491/1‑76 (MQ=255) aTGAACAAAAATTATGGTTGCCGAAGGGAGAAGTGCCATATGGCGAAGCGGCAACTTTAGATCTTGTCGGTCAgcg > 2:5219316/1‑76 (MQ=255) tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc > 2:3148745/1‑76 (MQ=255) tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc < 1:3148745/76‑1 (MQ=255) gAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcg > 1:240571/1‑76 (MQ=255) gAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcg < 1:5377791/76‑1 (MQ=255) aaCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcgc > 2:2938012/1‑76 (MQ=255) aaCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcgc > 1:2814592/1‑76 (MQ=255) aaCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcgc < 1:5127293/76‑1 (MQ=255) aaCAAAAATTATGGCTGCCACAGATAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGTGTCAgctcgc < 1:3667092/76‑1 (MQ=255) aaaaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACt > 1:1740122/1‑76 (MQ=255) aaaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTa > 1:3792462/1‑76 (MQ=255) aaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTAc > 1:3392197/1‑76 (MQ=255) aaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACa > 2:4808000/1‑76 (MQ=255) tATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAt < 2:5171684/76‑1 (MQ=255) tATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAt > 1:3113291/1‑76 (MQ=255) tATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAt > 1:271323/1‑76 (MQ=255) agggTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATc < 2:22208/74‑1 (MQ=255) tggtggCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg < 2:1702866/76‑1 (MQ=255) tGGTCGCCGATGGGCGAATTGCCATATGGAGAAGCGGTAAATGTCGATCTTGGGGGTCATCGCTCACTATAAATCg < 1:5795368/76‑1 (MQ=255) gCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGaa > 1:4659631/1‑76 (MQ=255) ccGAAGGGAGAATTGCCATATNGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt > 1:1300101/1‑76 (MQ=255) ccGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt < 2:3716541/76‑1 (MQ=255) cGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATg > 2:1573813/1‑76 (MQ=255) cGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATg > 2:2373671/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 2:4532792/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg < 2:2814592/76‑1 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 1:3432426/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 2:4987822/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 1:4584095/1‑76 (MQ=255) aaGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGc > 2:2210830/1‑76 (MQ=255) aaGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGc < 1:181142/76‑1 (MQ=255) aaGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGc > 2:3242533/1‑76 (MQ=255) aGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCa < 2:5804046/76‑1 (MQ=255) aGGGAGAATTGCCATATGGCGAAGCGGCAAATGTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCa > 1:2291288/1‑76 (MQ=255) gggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGGGCGCACTACAAATCGGCGAATGGCAg < 2:3323085/76‑1 (MQ=255) ggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAgg > 1:1446277/1‑76 (MQ=255) ggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAgg < 1:1768536/76‑1 (MQ=255) ggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAgg < 2:5664746/76‑1 (MQ=255) gagaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggg < 1:5211542/76‑1 (MQ=255) agaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAgggg < 1:4172924/76‑1 (MQ=255) gaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggggg > 2:5471227/1‑76 (MQ=255) gaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggggg < 1:2065491/76‑1 (MQ=255) aaTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGa > 1:4610323/1‑76 (MQ=255) aaTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGa < 1:161288/76‑1 (MQ=255) aaTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGa < 2:1480151/76‑1 (MQ=255) atggCCATATGGCGAAGCGGCAAATTTCGACCTTCGGGGCCAGCGCGCTCTACAAAGCGGCGCACGACAGGGGCaa > 2:597039/4‑76 (MQ=255) aTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGaa > 1:3276910/1‑76 (MQ=255) ttGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAc > 1:4155790/1‑76 (MQ=255) ttGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAc > 1:4913707/1‑76 (MQ=255) tGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAcc < 1:1432154/76‑1 (MQ=255) gCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCt > 2:4111480/1‑76 (MQ=255) gCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCt > 1:4528261/1‑76 (MQ=255) ccATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTg < 2:480561/76‑1 (MQ=255) ccATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTg < 1:1413878/76‑1 (MQ=255) ccATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTg > 2:5445155/1‑76 (MQ=255) ccATATGGCGAAGCGGAAAATTTCGATCTTGTGGGTCAGCGCGCACGACAAATCGGCGAATGGCAGCGGGAAACta > 2:2984878/1‑75 (MQ=255) cATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGt > 2:3952482/1‑76 (MQ=255) tatGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGttt > 2:1510838/1‑76 (MQ=255) atGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTg < 2:1300101/76‑1 (MQ=255) tGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTgg < 2:2878554/76‑1 (MQ=255) || GATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAACGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG > NC_000913/4194505‑4194645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |