| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,166,935 | 2 bp→CG | coding (312‑313/483 nt) | b3023 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,166,935 | 0 | G | C | 100.0% | 369.1 / NA | 139 | L105V (CTT→GTT) | b3023 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (57/82); total (57/82) | |||||||||||
| * | NC_000913 | 3,166,936 | 0 | C | G | 99.3% | 373.7 / NA | 139 | Q104H (CAG→CAC) | b3023 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (56/82); total (57/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
GCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAGCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC > NC_000913/3166867‑3167005 || gccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCgg > 1:2862217/1‑76 (MQ=255)gccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCgg < 2:126449/76‑1 (MQ=255)gccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCgg > 1:3144941/1‑76 (MQ=255)gccgcTTGCAGGCAGCCAGTGGCGAATGAGGCCCCACAACCTGTGTGTAATATCGTCCAGCTCCTCAACCTGCCgg > 2:696128/1‑76 (MQ=255) ccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCggg < 2:1398164/76‑1 (MQ=255) cgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGc > 2:1759720/1‑76 (MQ=255) gcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGcc > 2:4889714/1‑76 (MQ=255) ctggcaggcagCCAGTGGCGAATGATGCCGCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCa < 2:3991426/73‑1 (MQ=255) cTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCa < 2:3031590/76‑1 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc < 1:362962/76‑1 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc < 2:4150650/76‑1 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGGGCCGGGCCAc > 2:2061274/1‑76 (MQ=255) tGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACg < 2:1863995/76‑1 (MQ=255) tGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACg < 1:518595/76‑1 (MQ=255) tGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACg > 1:5203892/1‑76 (MQ=255) gcaggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACgg < 1:3357049/76‑1 (MQ=255) cgggcggcCAGTGTCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGc < 1:5403751/74‑1 (MQ=255) aggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCCACGTGGCGGGCCACCGcc > 2:1440096/1‑75 (MQ=255) gtagCCAGTGGCGAATGAGGCTCCATCCCGTGTGTGAAATATCGTGCAGCTCGCCAACGTGGCGGGCCACGGCATa < 2:3116158/74‑1 (MQ=255) gcagCCAGTCGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATa > 1:5812056/1‑76 (MQ=255) cagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATaa < 1:5143339/76‑1 (MQ=255) cagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATaa > 2:2725195/1‑76 (MQ=255) agCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAAc < 1:3258802/76‑1 (MQ=255) agCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAAc < 1:5456243/76‑1 (MQ=255) agCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAAc > 1:2326044/1‑76 (MQ=255) agCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAAc > 1:4140447/1‑76 (MQ=255) gCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCCCGGCATAACg > 2:530482/1‑76 (MQ=255) gCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACg < 1:1600674/76‑1 (MQ=255) ccAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGc < 1:5397597/63‑1 (MQ=255) ccAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGc > 2:5397596/1‑63 (MQ=255) ccAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACgc > 2:3397775/1‑75 (MQ=255) ccAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGt > 1:3991009/1‑76 (MQ=255) cAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATCACGTc > 2:5577950/1‑76 (MQ=255) cAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTc > 1:2308010/1‑76 (MQ=255) cAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTc < 2:3983630/76‑1 (MQ=255) cAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGAACAACGTc > 2:5181815/1‑76 (MQ=255) aGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTcc < 2:405461/76‑1 (MQ=255) aGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTcc > 2:3615756/1‑76 (MQ=255) gTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCa > 2:4146870/1‑76 (MQ=255) gTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCa > 1:1251962/1‑76 (MQ=255) tGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAc > 1:3403466/1‑76 (MQ=255) tGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAc < 1:2145343/76‑1 (MQ=255) gggCGAATGATGCCCCATACAGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAc < 1:4013561/75‑1 (MQ=255) gCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCg < 2:3276069/76‑1 (MQ=255) cGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAcccg > 1:529020/1‑74 (MQ=255) gAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGt > 2:3985464/1‑76 (MQ=255) aaTGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTa < 1:4678034/76‑1 (MQ=255) aaTGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTa < 2:3189479/76‑1 (MQ=255) atgatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGAATAACGCCCCCCgggac > 2:2205785/1‑73 (MQ=255) tgatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAg > 1:5144093/1‑76 (MQ=255) gatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCCGGCCACGGGATGAAGTACACCGGTaatg > 1:605243/1‑74 (MQ=255) atgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGtt < 1:1440096/76‑1 (MQ=255) tgCCCGATACCGTGTTTGGACTATCGTCCAGCACTCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTc < 1:2496357/76‑1 (MQ=255) tgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTc < 1:2966020/76‑1 (MQ=255) ccccATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAc > 1:2249480/1‑76 (MQ=255) ccccATACCGTGTGTGAAATAGAGTCCAGCGAGCCAACGGGGAGGGACACGGGAAAACATAGACCGGGAAGTTCAc > 1:698645/1‑76 (MQ=255) cATACCGTTTGTGAAATATCGTGCAGATCGCCAACGGGTCGGTCCACGGCACGACGTCCACCGGGAAGTACAACAt < 2:2018538/76‑1 (MQ=255) cATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAt < 1:3399046/76‑1 (MQ=255) cATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAt < 2:1962273/76‑1 (MQ=255) cATACCGTGTGTGAAATATCGCCCAGCCCCCCAACGTGGCGGGCCACGGCATAACGCCCCCCGGTAAGTTCACCAt < 1:4949312/76‑1 (MQ=255) aTACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAtt > 2:3607976/1‑76 (MQ=255) aTACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAtt < 1:1194532/76‑1 (MQ=255) tACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTg < 2:4786949/76‑1 (MQ=255) tACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTg < 2:2994915/76‑1 (MQ=255) tACCGTGTGTGAAAGATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTg < 2:2734090/76‑1 (MQ=255) cGTGTGTGGAATATCGTCCAGCCCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTc > 1:5214020/1‑76 (MQ=255) cGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTc < 1:1844622/76‑1 (MQ=255) cGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTc < 1:3431569/76‑1 (MQ=255) gtgtgtGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTca < 2:2397567/76‑1 (MQ=255) tgtgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTcac > 2:549681/1‑76 (MQ=255) tgtgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGCAAGTTCAACATGTCTcac > 1:2522502/1‑76 (MQ=255) gtgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTcaca < 1:4392116/76‑1 (MQ=255) tgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTcaccc > 2:3131360/1‑74 (MQ=255) gtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCCCGGCATAACGCTCCCCCGTAAGGTCCACATTGCTTCCAcc > 1:2758127/1‑76 (MQ=255) gtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACAcc > 2:5281517/1‑76 (MQ=255) tgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCa > 1:1097052/1‑76 (MQ=255) aaaTATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAATGCCCACACCCTa > 2:2178483/1‑76 (MQ=255) aaTATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATaa < 2:716158/76‑1 (MQ=255) atatAGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAc < 1:3080187/76‑1 (MQ=255) atCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGa < 1:3467964/76‑1 (MQ=255) ccgccAGCGCGCCAACGGGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAtt < 2:129520/73‑1 (MQ=255) cGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCGCGCCATAACGAtt < 2:2224638/76‑1 (MQ=255) cGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAtt < 1:320179/76‑1 (MQ=255) gTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTa < 2:3823882/76‑1 (MQ=255) tCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCCCACCATAACGAttct > 2:5173933/1‑74 (MQ=255) tCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAt < 1:3225031/76‑1 (MQ=255) tCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAt < 1:737905/76‑1 (MQ=255) tCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAt < 1:4608158/76‑1 (MQ=255) ccAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCGCCGGTAAGTTCACCATTGCTCACACCATAACGATTATc < 1:2151257/76‑1 (MQ=255) ccAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATc > 1:4109049/1‑76 (MQ=255) aGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCgg < 1:4870242/76‑1 (MQ=255) gCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCCTAACGATTATCggg < 2:3165596/76‑1 (MQ=255) cTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGa < 2:2668876/76‑1 (MQ=255) tCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGaa > 1:981195/1‑76 (MQ=255) tCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGaa < 2:2326044/76‑1 (MQ=255) cGCCAACGTGTCGTGCCGCGGCATCTCGTCCACCTGTCAGTTCACCATTGCTCACACCATAACGATTATCgggcat < 1:4482154/76‑4 (MQ=255) cGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAAt < 1:5462920/76‑1 (MQ=255) cGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAAt < 2:4483803/76‑1 (MQ=255) gCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATc < 2:5511040/76‑1 (MQ=255) gCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATc < 2:1240421/76‑1 (MQ=255) || GCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAGCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC > NC_000913/3166867‑3167005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |