| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,221,097 | 2 bp→GC | coding (824‑825/3093 nt) | b3076 → | evolved beta‑D‑galactosidase alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,221,097 | 0 | C | G | 98.7% | 371.2 / NA | 151 | T275S (ACG→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/2); major base G (77/70); minor base T (0/2); total (77/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,221,098 | 0 | G | C | 98.7% | 385.5 / NA | 150 | T275S (ACG→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (77/70); minor base T (0/2); total (77/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 151 total aligned reads displayed.
TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACG‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG > NC_000913/3221029‑3221167 || tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCt > 2:4277411/1‑76 (MQ=255)tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCt < 2:1406648/76‑1 (MQ=255) gATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCtt < 2:4788546/76‑1 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCtttt < 2:4502840/76‑1 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCtttt < 2:4764863/76‑1 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCtttt < 1:5816548/76‑1 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATCTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCtttt < 1:5657014/76‑1 (MQ=255) gggCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCtttt < 2:5595854/75‑1 (MQ=255) gCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTAc < 2:2310896/76‑1 (MQ=255) cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACt > 2:2486996/1‑76 (MQ=255) cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACt > 2:361848/1‑76 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTgc > 2:2325108/1‑75 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGt < 2:4573054/76‑1 (MQ=255) aaCGCGTGATGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGt < 2:2187627/76‑1 (MQ=255) aCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTc > 1:4134725/1‑76 (MQ=255) aCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTc < 2:4443753/76‑1 (MQ=255) aCGCGTGGGGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTc < 2:843926/76‑1 (MQ=255) cgcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCg < 2:1019947/76‑1 (MQ=255) cgcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCg > 1:2543239/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGa > 1:1024983/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGa > 1:3197238/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGa < 1:515413/76‑1 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGa > 1:5178587/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGa > 1:20194/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGa > 1:1607807/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑CTTGCTTTTACTGTCGa > 2:5365217/1‑76 (MQ=255) gtggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAAc < 2:4005579/76‑1 (MQ=255) gggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACa < 2:5210138/75‑1 (MQ=255) tgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGcc < 1:1131483/76‑1 (MQ=255) gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTCTTACTGTCGAACAGCCg > 1:2861489/1‑76 (MQ=255) gCACAGAAGCGCAATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGccn < 1:4464482/76‑2 (MQ=255) cacaTCACCGCCATTGATCATTTGGCAATTGAAAACCGGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCgc < 2:1585440/76‑1 (MQ=255) cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCgc > 2:1532230/1‑76 (MQ=255) cacaGCAGCGCCATTGATCATTGGGCAATTGAAAAACGGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCgc < 2:758999/76‑1 (MQ=255) cacaGCAGCGCAATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCgc < 2:3248307/76‑1 (MQ=255) agcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCcgccgc > 2:5813365/1‑76 (MQ=255) cagcGCCATTGATCATTTGGCAATGGAAAAACTGACCAGCGCCGGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCaa < 2:2799837/76‑1 (MQ=255) agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGCACAGCCGCCGCAAt > 2:5798793/1‑76 (MQ=255) agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAAt > 1:5634792/1‑76 (MQ=255) gcgcCATGGATCATTTGGCAATTGAAAACCTGACCAGCGCCAGC‑TTTGTTTTTACTGTCGAACAGCCGCAGCAATg < 2:2875315/76‑1 (MQ=255) cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATgg > 1:3935147/1‑76 (MQ=255) gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATggc > 2:4472441/1‑75 (MQ=255) gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt > 2:4100853/1‑76 (MQ=255) gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt < 1:245048/76‑1 (MQ=255) ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc > 1:5154287/1‑76 (MQ=255) ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc < 2:439165/76‑1 (MQ=255) cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca < 1:986136/76‑1 (MQ=255) cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca > 2:3933060/1‑76 (MQ=255) aTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTccg > 2:703462/1‑74 (MQ=255) aTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag > 1:2812795/1‑76 (MQ=255) ttGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACCGCCGCAGCCATGGCcaga > 2:141283/1‑75 (MQ=255) ttGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc > 1:1819908/1‑76 (MQ=255) tGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca < 1:3983218/76‑1 (MQ=255) tGATCATTTGACAATTGAAAAACTGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca < 1:2704475/76‑1 (MQ=255) gATCATTTGGAAATTGAAAAACTGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagcag < 1:1531837/76‑1 (MQ=255) aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa > 1:1745263/1‑76 (MQ=255) aTCATTTGGAAAGTGAAAAACGGACCAGCGCCGGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa < 2:2037227/76‑1 (MQ=255) cATTTGGCAATTGAAAAACTGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt < 1:1032591/76‑1 (MQ=255) cATTGGGCAATTAAAAAACTGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt < 1:4897402/76‑1 (MQ=255) tttGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcc > 2:3823441/1‑76 (MQ=255) ttgggCAATGGAAAAACTGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcc < 2:5259906/73‑1 (MQ=255) ttGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATccc > 2:4390641/1‑76 (MQ=255) ttGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGAAATGGTCAGCAGAATcac > 2:1589393/1‑74 (MQ=255) tGGCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGCCAGCAGAATcccc > 2:1342796/1‑76 (MQ=255) ggCATTTGAAAACCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt < 1:1521777/76‑1 (MQ=255) ggCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt > 1:1194714/1‑76 (MQ=255) ggCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt > 1:2029531/1‑76 (MQ=255) ggCAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt < 1:3967508/76‑1 (MQ=255) ggCAATTGAAAAACTGACCAGCGCCAGC‑TGTGCTTTTACTCTCGAACAGCCGCAGCAATGGGCAGCAGAATcaccc > 1:2318359/1‑75 (MQ=255) ggCAATTAAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGCCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt < 2:1510038/76‑1 (MQ=255) cgCACTTGAAAAGCTGTCCATCGCCATC‑TTTGTTTTTTCTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt < 1:3803934/75‑1 (MQ=255) cAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcccccta < 1:3001055/76‑4 (MQ=255) cAATTGAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa < 1:1219725/76‑1 (MQ=255) cAAGTGAAAACCGGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa < 2:3207264/76‑1 (MQ=255) aaTTGAAACCCCGCCCCGCGCCGGC‑TTTGCTTTTCCGGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAt < 2:306219/76‑1 (MQ=255) tgaaaaacCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt < 1:5022482/73‑1 (MQ=255) tGAAAAACGGCCCAGCGCCAGC‑TTTGTTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt < 2:5059465/76‑1 (MQ=255) gAAAAACTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt < 2:121364/76‑1 (MQ=255) aaaacaCTGACCCGCG‑CACGTTTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt < 1:4489348/75‑1 (MQ=255) aaaaaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGGCCGCAGAATCCCCTTATCtttc > 2:5016638/1‑75 (MQ=255) aaacCTGTCCGGCGCCACC‑TTTGCTTTTGCTGCCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc < 1:1367469/76‑1 (MQ=255) aaaaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc > 2:3436059/1‑76 (MQ=255) aaaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc > 1:1256889/1‑76 (MQ=255) aaaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc > 1:4717790/1‑76 (MQ=255) aaaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc > 1:781521/1‑76 (MQ=255) aaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCa > 2:3564954/1‑76 (MQ=255) aaCTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCa > 1:5406448/1‑76 (MQ=255) aCTGCCCAGCGCCAGC‑TTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt < 1:3576063/76‑1 (MQ=255) cTGACCAGCGCCAGC‑TTTGCTTTTCCTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc > 1:2724818/1‑76 (MQ=255) cTGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTCCCATc > 2:403284/1‑76 (MQ=255) tGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCCGCAGAATCCCCTTATCTTTACCATCt > 2:3942523/1‑76 (MQ=255) tGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt > 1:4277015/1‑76 (MQ=255) tGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt > 1:2646425/1‑76 (MQ=255) tGACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt > 2:2424770/1‑76 (MQ=255) gACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACATCCGCAGCAATGGGCAGCAGAATCCCCTTATCTATCCCATCTg > 1:3193110/1‑76 (MQ=255) gACCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg > 1:4067525/1‑76 (MQ=255) cccAGCGCCAGC‑TTTGCTTTTACGGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg < 2:2853936/75‑1 (MQ=255) aCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg > 1:2216006/1‑76 (MQ=255) aCCAGCGCCAGC‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg < 1:5036963/76‑1 (MQ=255) cgcCAGC‑TTTGCTTTTACTGCCAAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg < 2:1281971/76‑1 (MQ=255) || TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACG‑TTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG > NC_000913/3221029‑3221167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |