| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,019,860 | Δ2 bp | intergenic (+59/‑24) | b3836 → / → b3838 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,019,860 | 0 | C | . | 97.2% | 301.3 / 0.8 | 71 | intergenic (+59/‑25) | b3836/b3838 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base . (35/34); total (36/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
| * | NC_000913 | 4,019,861 | 0 | C | . | 97.1% | 295.2 / 2.0 | 71 | intergenic (+60/‑24) | b3836/b3838 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (34/34); minor base G (2/0); total (36/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGC > NC_000913/4019793‑4019924 || cAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc < 1:3251861/76‑1 (MQ=255)cAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc > 1:3236161/1‑76 (MQ=255) gggTGTATGCCGTGTTTGATAGCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc < 2:584176/75‑1 (MQ=255) aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc < 2:555619/76‑1 (MQ=255) aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc < 1:196673/76‑1 (MQ=255) ggTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCg > 1:927193/1‑76 (MQ=255) ggTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCg > 1:982388/1‑76 (MQ=255) gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc > 1:1025061/1‑76 (MQ=255) gtAATCCTTGTTTTATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCaa < 1:668938/76‑1 (MQ=255) gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCCTCATCGGCCTCG‑‑GCGTTCTGGGGCCCCac > 2:3733720/1‑75 (MQ=255) gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCac > 2:578217/1‑75 (MQ=255) gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCaa < 1:1143430/76‑1 (MQ=255) gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCaa > 1:3990131/1‑76 (MQ=255) tAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAc > 2:589231/1‑76 (MQ=255) tAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAc < 1:53469/76‑1 (MQ=255) aTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGa < 1:1682004/76‑1 (MQ=255) aTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGa > 2:505123/1‑76 (MQ=255) tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc > 2:1032271/1‑76 (MQ=255) cGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTg > 2:1112718/1‑76 (MQ=255) gtgtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGc > 1:3155575/1‑76 (MQ=255) gtgtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGc > 2:772030/1‑76 (MQ=255) gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCt > 1:3273630/1‑76 (MQ=255) gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCt > 2:2762757/1‑76 (MQ=255) tttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtg > 1:3372135/1‑76 (MQ=255) ttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt > 2:3797424/1‑76 (MQ=255) ttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt < 2:766460/76‑1 (MQ=255) ttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGCCCTCGGCG‑GTT‑TGGGTCCTCAACTACTGCCtgg > 2:3834836/1‑75 (MQ=255) tGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgtg > 1:3181286/1‑76 (MQ=255) tGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgtg < 1:1112719/76‑1 (MQ=255) gATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGCCTGCCTGCgg > 2:716030/1‑76 (MQ=255) gATAGCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg < 2:3602957/76‑1 (MQ=255) atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGc < 1:1511002/76‑1 (MQ=255) atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGc > 2:2146428/1‑76 (MQ=255) atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGCCCGCAACGACTGCCTGTGGc > 2:3833822/1‑76 (MQ=255) tatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑g < 1:4221223/76‑1 (MQ=255) atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg > 2:890775/1‑76 (MQ=255) atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg > 1:3614128/1‑76 (MQ=255) atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg < 1:3237733/76‑1 (MQ=255) atCGGTTTGAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gg < 2:1157755/76‑1 (MQ=255) tgggTTTTAGCGAACGGCTATTGGTGTTCATCATCGGCCGCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGt < 2:172120/74‑1 (MQ=255) cGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gggg > 1:4818264/1‑74 (MQ=255) cGGTGTTAGCGAACGGCTATGGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTa < 2:4078494/76‑1 (MQ=255) ggTTTTAGCGAACTGCTTTTGGTGTTCATCATCGGCCGCGGCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTaa < 2:7369/76‑1 (MQ=255) ggTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑gggga > 1:2132906/1‑73 (MQ=255) ggTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTaa < 1:2638067/76‑1 (MQ=255) ggTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTaa < 1:3323659/76‑1 (MQ=255) ttttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGaaaa > 1:1776391/1‑76 (MQ=255) tttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTaaaaa < 2:3631461/76‑1 (MQ=255) tttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGaaaaa > 1:3570339/1‑76 (MQ=255) ttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGaaaaaa > 1:657038/1‑75 (MQ=255) tAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACg < 2:3129629/76‑1 (MQ=255) tAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGaaaaaag > 1:4116726/1‑74 (MQ=255) aGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACgg < 1:679393/76‑1 (MQ=255) aGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGAAAAAAgg > 1:3137405/1‑76 (MQ=255) ggAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCGGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTa < 2:4325069/75‑1 (MQ=255) gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGTAAAAACgcag > 2:3056376/1‑73 (MQ=255) gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGGAAAAAGGGAg > 1:1766899/1‑76 (MQ=255) aaCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGc < 1:2081295/76‑1 (MQ=255) aCTGCTATTGTTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCg < 1:1068401/76‑1 (MQ=255) aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCg < 1:4470530/76‑1 (MQ=255) gtATTGGTGTTCATCATCTGCCTCG‑‑TCTGTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCt < 2:3570339/75‑1 (MQ=255) cTATTGTTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCt < 1:2044249/76‑1 (MQ=255) tATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGGAAAAAGGTAGggggggg > 1:2687271/1‑73 (MQ=255) tATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGAAAAAAGGTAGCGGGGTg > 1:2682848/1‑76 (MQ=255) aTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGGAAAAAATGGAGCGGGGTgg > 1:469073/1‑76 (MQ=255) tgtggTCATCGGCGGCCTCGTCG‑‑TTCGGGGGCGGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGAtt < 2:1408586/75‑1 (MQ=255) gtgtTCGTCTGCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTc < 2:4596585/76‑1 (MQ=255) tcagcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCgcgtt < 2:4089667/76‑1 (MQ=255) catcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCgcgttg < 2:2094208/76‑1 (MQ=255) catcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCgcgttg < 1:818128/76‑1 (MQ=255) atcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCgcgttgc < 1:1319699/76‑1 (MQ=255) || CAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGC‑GGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGC > NC_000913/4019793‑4019924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |