| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,051,144 | Δ1 bp | intergenic (+146/+305) | hemN → / ← glnG | O2‑independent coproporphyrinogen III oxidase/GlnG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,051,143 | 0 | C | . | 98.8% | 257.0 / ‑3.1 | 84 | intergenic (+145/+306) | hemN/glnG | O2‑independent coproporphyrinogen III oxidase/GlnG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (43/40); minor base T (0/1); total (43/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC > NC_000913/4051069‑4051212 | tGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGca < 2:3877512/76‑2 (MQ=255) aTGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGcatc > 1:1715395/1‑73 (MQ=255) aTGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGcatc < 1:2619220/76‑4 (MQ=255) gcgcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTg < 1:2654158/76‑1 (MQ=255) cgcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGAGTTGACGCCGCATCAGG‑CATCTGa > 1:582541/1‑76 (MQ=25) gcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg > 1:3405857/1‑76 (MQ=255) gcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg < 2:2271789/76‑1 (MQ=255) gcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg < 1:965992/76‑1 (MQ=255) gcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg < 1:3285434/76‑1 (MQ=255) cTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGc > 1:2699710/1‑76 (MQ=255) gggCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCat < 1:1437702/76‑1 (MQ=255) gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatga < 2:723146/76‑1 (MQ=255) gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatga < 2:3989893/76‑1 (MQ=255) aacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCCTCAGG‑CATCTGACCatgatc > 2:168289/1‑75 (MQ=21) acaaAACCTGTTGAACTTATTGCTTTTGTAGGCCGGATAATGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGc < 2:3636233/76‑1 (MQ=25) acaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGc > 2:3997036/1‑76 (MQ=255) aaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTg < 2:4831980/76‑1 (MQ=255) aaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGa > 1:4052777/1‑76 (MQ=255) aaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGa < 1:1340798/76‑1 (MQ=255) agcTGTTGAGATTATTGCTTTTGTATGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGTGCATGATGCCTGAt < 2:4242455/74‑1 (MQ=21) aCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGAt > 1:3576076/1‑76 (MQ=255) ccTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATg < 2:2677339/76‑1 (MQ=255) cTGTTGCAATTATTGCTTTTGTAGTGCGGGTAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgc < 2:3416694/76‑1 (MQ=21) cTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgc < 1:1892130/76‑1 (MQ=255) tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGG‑CATCTGACCCTGCTGCATGATgcg > 2:681713/1‑76 (MQ=17) tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcg > 2:2371839/1‑76 (MQ=255) tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcg > 2:654059/1‑76 (MQ=255) tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcg < 2:395081/76‑1 (MQ=255) gTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcgg < 1:4415664/76‑1 (MQ=255) gTTGAAATTATTGCTTGTGTAGGCCGGATAAGGCGGTTACGCCGCATCAGG‑CATCTGAGCATGAGGCCTGATgcgg < 1:4429157/76‑1 (MQ=25) ttGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggc < 2:4296059/76‑1 (MQ=255) gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc < 1:2178364/76‑2 (MQ=255) gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc < 2:1655209/76‑2 (MQ=255) aaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgctg < 2:2082580/76‑4 (MQ=21) attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg < 2:1749076/76‑1 (MQ=18) attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg < 2:2723264/76‑1 (MQ=21) tggttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgcg < 2:1253639/73‑1 (MQ=18) gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGt > 1:2275095/1‑76 (MQ=21) cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc < 1:1701747/76‑1 (MQ=21) ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGGCt > 1:2443486/1‑76 (MQ=18) ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCCGCGCTCGCCGCGTCt > 2:410167/1‑76 (MQ=17) tttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCtt > 1:3874497/1‑76 (MQ=18) tttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCtt > 1:3966757/1‑76 (MQ=18) ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTa > 1:3672564/1‑76 (MQ=18) tGTAGGTCGGCTAATGCGTTTACTCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAt < 1:1146967/76‑1 (MQ=17) tGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAt < 1:1169290/76‑1 (MQ=18) tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCa < 2:2961998/76‑1 (MQ=18) aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg > 1:3455993/1‑76 (MQ=18) aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg > 1:1998249/1‑76 (MQ=18) aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg > 2:2907271/1‑76 (MQ=17) ggCGGGATGGGGCGTTGACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAgg < 2:257473/76‑1 (MQ=14) ggCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAgg < 1:4817138/76‑1 (MQ=17) ggCCGAATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGTGGCGCTGGTCGCGTCTTATCAgg < 1:4821296/76‑1 (MQ=17) gCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGc < 2:3917072/76‑1 (MQ=17) cGGATAAGGCGTTTACGCCGCATCA‑GTCATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCt < 2:1888872/76‑1 (MQ=17) gATAGGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc < 2:426568/76‑1 (MQ=14) aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc < 2:3029404/76‑1 (MQ=17) aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt > 2:2724954/1‑76 (MQ=18) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct > 1:2860202/1‑76 (MQ=18) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct > 2:2968455/1‑76 (MQ=18) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct > 2:3002451/1‑76 (MQ=18) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGGCGCGCCTTCTCCGCGCCACGACtttgc > 2:1746167/1‑74 (MQ=9) tttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGCCTTtctc > 2:444669/1‑76 (MQ=17) tttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtctc < 2:2496165/76‑1 (MQ=18) tttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtctc < 2:3650588/76‑1 (MQ=18) tttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCCACGACTTtctc > 1:4753504/1‑76 (MQ=18) tttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGAGCTGGTCGCGTCTTATCAGGCCTACGACCTtctc > 2:566171/1‑76 (MQ=18) tttACGCCGCATCAGG‑CAGCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtctc < 2:2760819/76‑1 (MQ=18) ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCCGGGCTACGACTTTCTcc > 2:878129/1‑76 (MQ=18) ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTcc > 1:1396638/1‑76 (MQ=21) tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg > 2:1792341/1‑76 (MQ=21) tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg > 2:1757836/1‑76 (MQ=21) aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc > 2:2821285/1‑76 (MQ=21) aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc < 2:1594107/76‑1 (MQ=18) cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa > 2:3980126/1‑76 (MQ=21) cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa < 1:673766/76‑1 (MQ=21) gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt > 1:64683/1‑76 (MQ=21) gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt > 1:4782650/1‑76 (MQ=21) gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt > 2:76887/1‑76 (MQ=21) cgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc < 1:3355365/76‑1 (MQ=21) gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCg > 1:647711/1‑76 (MQ=25) cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCCTcccg > 2:284885/1‑74 (MQ=21) cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg > 2:277183/1‑76 (MQ=25) aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc > 2:3559681/1‑76 (MQ=25) aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc > 2:3289364/1‑76 (MQ=25) aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc > 2:1436396/1‑76 (MQ=25) aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc > 1:1465514/1‑76 (MQ=25) | TGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC > NC_000913/4051069‑4051212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |