| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,477,041 | Δ1 bp | intergenic (‑151/‑269) | b4256 ← / → yjgN | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,477,041 | 0 | G | . | 98.8% | 361.9 / ‑3.1 | 81 | intergenic (‑151/‑269) | b4256/yjgN | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (33/47); total (33/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC > NC_000913/4476972‑4477110 | gACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTa > 1:1651177/1‑76 (MQ=255) gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCt < 1:702440/76‑1 (MQ=255) tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGGCGCGGGCGATAGC‑AGGATTATCCTg > 2:2464436/1‑76 (MQ=255) tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTg < 1:3636107/76‑1 (MQ=255) tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTg < 1:1590341/76‑1 (MQ=255) gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑CGGATTATCCTGc > 2:4321434/1‑76 (MQ=255) gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGc < 1:1847279/76‑1 (MQ=255) tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCa < 2:1972087/76‑1 (MQ=255) tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCa < 2:4601004/76‑1 (MQ=255) gCCTTTATCAGCGGTAAGCCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGc < 2:2368793/76‑1 (MQ=255) gCCGTTGTCAGCGGTGAGACCGTATTCGGCGGATGCCTGACGCATGACGCGGGCGATAGC‑AGGCTTATCCTGCAGc < 2:808659/76‑1 (MQ=255) cTTTATCAGCGGTAAGACCGTATGCGGCGGATACCTGACGAATGCCGCGGGCGATCTC‑AGGCTTATCCTGCAGCGt < 1:556254/76‑1 (MQ=255) tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAg < 1:4321434/76‑1 (MQ=255) tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAg < 2:2382660/76‑1 (MQ=255) aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAgg < 1:2504283/76‑1 (MQ=255) tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc > 2:3837435/1‑76 (MQ=255) tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc > 2:3108474/1‑76 (MQ=255) tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc > 1:1057778/1‑76 (MQ=255) tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc < 2:4233097/76‑1 (MQ=255) tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc > 1:1533975/1‑76 (MQ=255) cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcg < 1:2353991/76‑1 (MQ=255) gCGGTGAGGCCGTGTGCGGCGGATGCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCGGCGTCAGGcgac < 2:2653779/76‑1 (MQ=255) gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac < 1:1556703/76‑1 (MQ=255) gCGGGAAGGCCGTGTTCGGGGGGTGCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac < 2:332780/76‑1 (MQ=255) cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑g > 1:3437306/1‑76 (MQ=255) ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gc > 1:3004857/1‑75 (MQ=255) gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTAc > 1:1564502/1‑76 (MQ=255) aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa < 1:2257034/76‑1 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg < 1:3481326/76‑1 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg < 2:1761506/76‑1 (MQ=255) cggaTTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAgg < 2:3704070/73‑1 (MQ=255) cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGTGAC‑GCATTACAgg < 2:4023304/76‑1 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAggg > 1:634606/1‑75 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGt > 2:2972790/1‑76 (MQ=255) aTTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGa > 2:3960893/1‑76 (MQ=255) tCGGCGGATACCTGGCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAtt < 2:446383/76‑1 (MQ=255) tCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAtt < 1:3837435/76‑1 (MQ=255) cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTg < 2:3024876/76‑1 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc > 2:2343336/1‑76 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc < 1:3298575/76‑1 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc < 2:1478775/76‑1 (MQ=255) gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcg > 1:1747763/1‑76 (MQ=255) cggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcgg < 1:1159088/76‑1 (MQ=255) cggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcgg > 1:875595/1‑76 (MQ=255) ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcggc < 2:4809603/76‑1 (MQ=255) ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcggc < 2:3605509/76‑1 (MQ=255) ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTccggc > 2:176809/1‑76 (MQ=255) aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGgcgc < 1:2685434/76‑1 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa > 1:393854/1‑76 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa > 1:2972853/1‑76 (MQ=255) ccTGACGAGTCAGGCGGGCGAGAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGATGATTGCGGCGCAAt < 1:3320466/76‑1 (MQ=255) cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg > 2:2731245/1‑76 (MQ=255) tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGTGAC‑GCATTACAGGTGATTGCGGCGCAATGt > 2:3424175/1‑76 (MQ=255) tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGt < 2:2725327/76‑1 (MQ=255) gactGACGCGGGCGATAG‑GTGGATTATCCTGCAGCGTCAGGCGAC‑GCATTCCAGGAGATTGCGGCGCAATGTTCtt < 2:3000782/73‑1 (MQ=255) gAATGGCGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt < 2:4700800/76‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt < 2:1636352/76‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt < 1:2963121/76‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt > 1:1819241/1‑76 (MQ=255) gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt > 1:4534887/1‑76 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc < 1:2576690/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc > 2:2520471/1‑76 (MQ=255) aTGACGCGGGCGACAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa > 2:1396884/1‑76 (MQ=255) tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAt > 2:829264/1‑76 (MQ=255) aCGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAg < 2:2572941/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 2:3088664/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:3656851/76‑1 (MQ=255) gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt > 2:3755647/1‑76 (MQ=255) gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt > 1:3697519/1‑76 (MQ=255) gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt > 1:1711655/1‑76 (MQ=255) cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt > 2:706424/1‑76 (MQ=255) cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt < 1:3367764/76‑1 (MQ=255) catgCGATAGC‑AGGATTATCCGGCAGCGTCAGGCGAC‑GCATGACAGGTGATTGCGGCGCAAGGTTATTCATAGttt < 1:3392416/73‑1 (MQ=255) gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa > 1:1845650/1‑76 (MQ=255) gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa > 1:2323139/1‑76 (MQ=255) gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa < 2:3517267/76‑1 (MQ=255) ggCGATAGC‑AGGGTTATCCTGCAGCGTCAGGCGACGG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 2:4580832/76‑1 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa > 2:248973/1‑76 (MQ=255) gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc < 1:1564600/76‑1 (MQ=255) cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTGCAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc < 1:3843536/76‑1 (MQ=255) gATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc < 1:537851/76‑1 (MQ=255) | GACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC > NC_000913/4476972‑4477110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |