| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,429,527 | Δ1 bp | coding (40/510 nt) | yrdD ← | putative DNA topoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,429,526 | 0 | C | . | 98.1% | 461.4 / 0.5 | 104 | coding (41/510 nt) | yrdD | putative DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (42/60); minor base G (1/1); total (43/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC > NC_000913/3429451‑3429597 | gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc < 2:3091068/76‑1 (MQ=255) aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg < 2:2442536/76‑2 (MQ=255) aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg > 2:3989078/1‑75 (MQ=255) aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg > 2:4054335/1‑75 (MQ=255) aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg < 2:2522561/76‑2 (MQ=255) aGATTTCAGAGGGCGGGCGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga < 2:4366882/76‑3 (MQ=255) aGATTTCAGAGGGCGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga < 2:4442725/76‑3 (MQ=255) tttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATa < 2:3670684/76‑1 (MQ=255) cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAca < 2:1616513/76‑2 (MQ=255) tgagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa > 2:1656129/2‑76 (MQ=255) agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa > 2:1051565/1‑76 (MQ=255) agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa < 2:1275813/76‑1 (MQ=255) agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa < 1:2594833/76‑1 (MQ=255) agagGACGGACGTAGTCAAACGCCGGCTACTGTGAGCATCCAAGAAACGGACCGTGTTTACCGGAT‑CGCATAACCa > 1:1580767/1‑76 (MQ=255) ggggacggccGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg < 2:1865274/74‑1 (MQ=255) gagGACGGACGTAGTGACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg < 2:2540286/76‑1 (MQ=255) gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg < 2:2493499/76‑1 (MQ=255) gagGACGGACGTAGCCACCCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg < 1:434465/76‑1 (MQ=255) agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt > 1:4200859/1‑76 (MQ=255) gacggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc < 1:1439126/76‑1 (MQ=255) gcggacgTAGGCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa < 2:376132/75‑1 (MQ=255) acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa < 2:3623236/76‑1 (MQ=255) acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa < 1:657818/76‑1 (MQ=255) gcgTAGTCACGCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGccc < 2:494168/75‑1 (MQ=255) cgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc > 2:2070934/1‑76 (MQ=255) cgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc < 2:1341689/76‑1 (MQ=255) gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg > 1:3840691/1‑76 (MQ=255) gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg > 2:3693189/1‑76 (MQ=255) tCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACt < 1:2624687/76‑1 (MQ=255) cacaCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt > 2:2760846/1‑76 (MQ=255) acacGCCGGATACTGTGAGTATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt > 1:63755/1‑76 (MQ=255) cacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg < 1:2945837/76‑1 (MQ=255) ccGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg < 2:34328/75‑1 (MQ=255) acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg < 1:2550789/76‑1 (MQ=255) cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg < 1:2954135/76‑1 (MQ=255) cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg < 1:2945802/76‑1 (MQ=255) gCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGc > 2:3030847/1‑76 (MQ=255) gCCGGATACTGTGAGCATCAAAGAAACGGACAGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGc < 2:4001268/76‑1 (MQ=255) ccGGATACTGGGAGCATCCAAGAAACGGACCGTGTTTCCAGGA‑GCGAATAAACAGTCAAGCCACGCGCTTTGGGCa > 2:805649/1‑76 (MQ=255) cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg > 1:1350876/1‑76 (MQ=255) cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg > 1:3926102/1‑76 (MQ=255) cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg > 1:2118449/1‑76 (MQ=255) ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAgg < 1:2261137/76‑1 (MQ=255) ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAgg > 1:3939920/1‑76 (MQ=255) gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt > 2:4196189/1‑68 (MQ=255) gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa > 1:733210/1‑76 (MQ=255) gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa < 2:1622076/76‑1 (MQ=255) aTACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc < 1:2807879/76‑1 (MQ=255) acggtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTc < 2:4510899/73‑1 (MQ=255) aCTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTc < 1:3062110/76‑1 (MQ=255) aCTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTc > 1:4553890/1‑76 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat > 1:354237/1‑76 (MQ=255) gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt < 2:3260735/76‑1 (MQ=255) gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt < 1:4204256/76‑1 (MQ=255) gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattat > 2:3268436/1‑76 (MQ=255) gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattan < 1:2016530/76‑2 (MQ=255) aGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt < 2:396249/76‑1 (MQ=255) gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta > 1:2207864/1‑76 (MQ=255) gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta < 1:4054335/76‑1 (MQ=255) gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta < 1:1336589/76‑1 (MQ=255) cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc > 1:4764198/1‑76 (MQ=255) cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc < 1:4725190/76‑1 (MQ=255) cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc > 2:2479793/1‑76 (MQ=255) tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGc > 1:4756987/1‑76 (MQ=255) tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGc < 2:3486277/76‑1 (MQ=255) tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGc < 2:63755/76‑1 (MQ=255) ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCa < 1:1065215/76‑1 (MQ=255) ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCa > 1:3973421/1‑76 (MQ=255) cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc > 1:863831/1‑76 (MQ=255) cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc > 2:2320208/1‑76 (MQ=255) aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc < 1:3411780/76‑1 (MQ=255) aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc > 1:597528/1‑76 (MQ=255) aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg > 1:2519480/1‑76 (MQ=255) aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACGTTGGGCAGGACTCATTATTACGCACCg < 2:1394111/76‑1 (MQ=255) gAAAGGGACCGTGTTTCCCGGAT‑CGATTAACCAGTTCAGCCCCGCTCTTTGGGCAGGACTCATTATTACGCAcctt < 1:2287572/76‑3 (MQ=255) gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt > 2:801304/1‑76 (MQ=255) gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt < 1:1656130/76‑1 (MQ=255) gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt > 1:2472971/1‑76 (MQ=255) gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt > 1:441913/1‑76 (MQ=255) gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt < 2:3599516/76‑1 (MQ=255) agcGGACCGTGTTTCCCGTAT‑CGAATAACCAGTTCGGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTgc < 1:3771689/74‑2 (MQ=255) aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAACgcca > 2:731438/1‑73 (MQ=255) aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGaa < 2:1040533/76‑1 (MQ=255) ggACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa > 2:3368567/1‑76 (MQ=255) gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg < 1:3977346/76‑1 (MQ=255) gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATCATTACGCACCGCGAACAg > 2:3623375/1‑76 (MQ=255) gACCGTGTTGCCCGGAT‑CGAATAACCAGTGCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg < 1:3984217/76‑1 (MQ=255) gACCGTGATTCCCGGA‑GCGAATATCCAGTTCAGCCCCGCACTTTGGGCAGGGGTCATTATTACGCACCGTGAACAg < 2:2437023/76‑1 (MQ=255) aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGGCTCATTATTACGCACCGTGAACAGt > 1:4591120/1‑76 (MQ=255) aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAcggt > 1:1230760/1‑73 (MQ=255) ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTg < 1:3874481/76‑1 (MQ=255) ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTg < 1:1206201/76‑1 (MQ=255) ggtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc < 1:252025/75‑1 (MQ=255) cGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc < 1:3596924/76‑1 (MQ=255) gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCGCTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt < 1:2831368/76‑1 (MQ=255) tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTgct > 2:1578214/1‑74 (MQ=255) ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt > 2:1535025/1‑76 (MQ=255) tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt > 2:1138805/1‑76 (MQ=255) tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt < 2:1734861/76‑1 (MQ=255) cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGc < 2:3263163/76‑1 (MQ=255) | GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC > NC_000913/3429451‑3429597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |