Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,573,700 +C intergenic (+403/+997) yhgN → / ← gntK hypothetical protein/gluconokinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,573,7001.C100.0% 335.4 / NA 135intergenic (+403/+997)yhgN/gntKhypothetical protein/gluconokinase 2
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (74/61);  total (74/61)

Only 100 of 140 total aligned reads displayed.

GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAC  >  NC_000913/3573625‑3573769
                                                                            |                                                                     
gAACCCTGAATGATGCGCACTGCAGCCGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                        >  2:1080923/1‑76 (MQ=255)
 aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgc                                                                       >  2:2120541/1‑75 (MQ=255)
  aCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgct                                                                      <  1:3467824/76‑3 (MQ=255)
      tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGc                                                                  <  2:1205286/76‑1 (MQ=255)
       gAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGcc                                                                 >  2:2846782/1‑76 (MQ=255)
        aaTGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACGCTTCGccc                                                                >  1:20898/1‑76 (MQ=255)
         atgaggCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCa                                                               <  2:870482/76‑1 (MQ=255)
           gatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGCTCACGCCTCGCCCAGt                                                             >  2:2104314/1‑76 (MQ=255)
            atgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTg                                                            <  1:4029849/76‑1 (MQ=255)
              gcgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCCTGCCGGTTAACGCTTCGCCCAGTGcc                                                          >  2:3221534/1‑76 (MQ=255)
              gcgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGcc                                                          >  2:3962243/1‑76 (MQ=255)
               gcgaCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCg                                                         <  2:3855896/73‑1 (MQ=255)
               cgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCg                                                         <  1:2314871/76‑1 (MQ=255)
                gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGGCgg                                                        >  2:3222777/1‑76 (MQ=255)
                gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCgg                                                        <  1:866209/76‑1 (MQ=255)
                 cACTGCAGCTGCCAGCACGAAGCAGGTGATAGCACTCGGCAGGCCTGTGTCGGTTCCCGCTTCGCCCAGTGCCGGa                                                       <  1:1921800/76‑1 (MQ=255)
                 cACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGa                                                       >  1:2021739/1‑76 (MQ=255)
                  aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAc                                                      >  1:2912479/1‑76 (MQ=255)
                   cTGCGGCGGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAcc                                                     <  2:4632692/76‑1 (MQ=255)
                   cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAcc                                                     >  1:4768111/1‑76 (MQ=255)
                    tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCt                                                    <  1:2398414/76‑1 (MQ=255)
                     gccgCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTa                                                   <  1:2152488/73‑1 (MQ=255)
                     ccAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTa                                                   <  2:659129/75‑1 (MQ=255)
                      cAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGTCCTAc                                                  <  1:3741664/76‑1 (MQ=255)
                      cAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                  <  1:2282138/76‑1 (MQ=255)
                       aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAcc                                                 >  2:915526/1‑75 (MQ=255)
                       aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                 <  2:3431303/76‑1 (MQ=255)
                       aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                 >  2:2465312/1‑76 (MQ=255)
                       aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                 >  1:1916646/1‑76 (MQ=255)
                       aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                 <  1:2645446/76‑1 (MQ=255)
                        gcggCCAGCACAAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACGCTTCGCCCAGTGCCGGACCTACGc                                                <  2:4749154/73‑1 (MQ=255)
                        gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGc                                                <  2:3094903/76‑1 (MQ=255)
                        gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGc                                                >  1:4689519/1‑76 (MQ=255)
                         cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGcc                                               <  1:783798/76‑1 (MQ=255)
                         cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGcc                                               <  1:150350/76‑1 (MQ=255)
                           gcgaGCGCGAAGGAGGTGATAGCAATCGGCGGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCag                                             <  2:4623003/73‑1 (MQ=255)
                           gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCag                                             >  1:2865357/1‑76 (MQ=255)
                             cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCagag                                           >  1:3486359/1‑76 (MQ=255)
                             cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCagac                                           >  2:2466865/1‑75 (MQ=255)
                              aGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                          <  1:4849667/76‑1 (MQ=255)
                                cACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCa                                        >  1:3145615/1‑76 (MQ=255)
                                 aCGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                       <  1:1194132/76‑1 (MQ=255)
                                  cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAc                                      >  1:3676307/1‑76 (MQ=255)
                                   gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                     >  1:2133833/1‑76 (MQ=255)
                                   gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                     >  1:1179641/1‑76 (MQ=255)
                                   gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                     >  2:4049400/1‑76 (MQ=255)
                                    aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGCCCGACCTAAGCCAGAGTCAAccc                                    >  2:4022465/1‑75 (MQ=255)
                                    aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                    >  1:4736697/1‑76 (MQ=255)
                                     aGCAGGGGATAGCAATCGGCAGGCCCATGCCGTTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAg                                   <  1:3297019/76‑1 (MQ=255)
                                     aGCAGGGGATAGCAATCGGCAGGCCCAGGCCGGTTAACGCTTCGCCCAGTGCCCGACCCACGCCATAGTCAACCac                                   >  2:823805/1‑75 (MQ=255)
                                      gCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGc                                  >  1:598688/1‑76 (MQ=255)
                                      gCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGc                                  >  1:2340555/1‑76 (MQ=255)
                                        aGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAc                                >  2:1845325/1‑76 (MQ=255)
                                         ggTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAcc                               >  1:2037575/1‑76 (MQ=255)
                                          gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCCCCt                              >  2:2718256/1‑76 (MQ=255)
                                          gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                              >  1:3943721/1‑76 (MQ=255)
                                          gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                              >  1:1083621/1‑76 (MQ=255)
                                           tGATAGCAATCTGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                             <  1:1067208/76‑1 (MQ=255)
                                           tGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCCGCCCCTg                             >  2:761838/1‑76 (MQ=255)
                                               aGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTg                         <  1:915527/76‑1 (MQ=255)
                                               aGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTg                         <  1:2948380/76‑1 (MQ=255)
                                                gCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                        <  1:2742590/76‑1 (MQ=255)
                                                 cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGaa                       <  1:1527082/76‑1 (MQ=255)
                                                 cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGaa                       <  2:823959/76‑1 (MQ=255)
                                                 cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGaa                       >  1:4177188/1‑76 (MQ=255)
                                                 cAAGCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGaa                       <  2:378512/76‑1 (MQ=255)
                                                  aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                      >  2:1257332/1‑76 (MQ=255)
                                                  aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                      >  1:4448894/1‑76 (MQ=255)
                                                   aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGCCCGACCCACCCCAGAGTCAACCAGCACCCGTTTGAaca                     >  2:934873/1‑76 (MQ=255)
                                                   aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAaca                     <  2:3160982/76‑1 (MQ=255)
                                                    tCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                    <  1:1026975/76‑1 (MQ=255)
                                                      ggCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgc                  >  2:4347856/1‑76 (MQ=255)
                                                       gCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCCCCTGTTTGAACAcgcc                 <  1:934763/76‑1 (MQ=255)
                                                       gCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgcc                 <  1:2230463/76‑1 (MQ=255)
                                                         aGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc               >  2:3131048/1‑76 (MQ=255)
                                                         aGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc               >  1:850807/1‑76 (MQ=255)
                                                          ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc              <  2:4259368/76‑1 (MQ=255)
                                                          ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc              <  1:1321266/76‑1 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCGCCTGTTTGAACACGccgccc             <  1:2928981/76‑1 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc             <  2:2859244/76‑1 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc             >  1:3306347/1‑76 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc             >  1:2224042/1‑76 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc             <  1:2866519/76‑1 (MQ=255)
                                                             cgaTGCGGGGTAACGCTGCGCCCGGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc           <  2:108374/74‑1 (MQ=255)
                                                             ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc           >  2:3714518/1‑76 (MQ=255)
                                                             ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc           >  1:2604446/1‑76 (MQ=255)
                                                             ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc           >  1:3488934/1‑76 (MQ=255)
                                                             ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc           >  2:2618173/1‑76 (MQ=255)
                                                               aTGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc         >  2:1630009/1‑76 (MQ=255)
                                                                 gcgggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCa       <  2:2186054/73‑1 (MQ=255)
                                                                 gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCa       <  1:3037335/76‑1 (MQ=255)
                                                                  ccGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCaa      >  2:3517906/1‑76 (MQ=255)
                                                                  ccGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCaa      >  2:3536915/1‑76 (MQ=255)
                                                                    ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc    >  2:3868350/1‑76 (MQ=255)
                                                                    ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc    <  1:4085060/76‑1 (MQ=255)
                                                                    ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc    >  2:1268878/1‑76 (MQ=255)
                                                                     gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa   >  1:2785081/1‑76 (MQ=255)
                                                                     gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa   <  1:2198152/76‑1 (MQ=255)
                                                                     gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa   >  2:2725207/1‑76 (MQ=255)
                                                                      ttAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAc  <  2:1468857/76‑1 (MQ=255)
                                                                            |                                                                     
GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAC  >  NC_000913/3573625‑3573769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑