| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,686,775 | +T | intergenic (‑23/+9) | b3530 ← / ← yhjM | putative oxidoreductase subunit/putative endoglucanase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,686,775 | 1 | . | T | 98.4% | 372.2 / ‑4.1 | 121 | intergenic (‑23/+9) | b3530/yhjM | putative oxidoreductase subunit/putative endoglucanase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base T (49/70); minor base C (2/0); total (51/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG > NC_000913/3686700‑3686844 | aCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCg < 2:3116858/76‑1 (MQ=255)aCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCg > 1:64769/1‑76 (MQ=255)aCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCg < 1:3240905/76‑1 (MQ=255) cTTTCTCCAGCAACTGTTGCGGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgt < 2:3741696/76‑2 (MQ=255) cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgt > 2:3254022/1‑75 (MQ=255) ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtg < 1:2946132/76‑3 (MQ=255) tGCTCCGGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtga < 2:578137/76‑4 (MQ=255) tGCGCCAGCAACTGTGGCTGAGGGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtga < 2:1265140/76‑4 (MQ=255) tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAata > 2:799461/1‑76 (MQ=255) cAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATg > 2:4159985/1‑76 (MQ=255) cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGttt > 1:4712250/1‑76 (MQ=255) aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTa < 2:4126894/76‑1 (MQ=255) aCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAg < 1:4179770/76‑1 (MQ=255) cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgt < 1:1567310/76‑1 (MQ=255) cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgt > 1:2164054/1‑76 (MQ=255) tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtg < 2:769684/76‑1 (MQ=255) tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtg > 2:2744530/1‑76 (MQ=255) ggtGCTGGGGGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtgt < 2:2217821/74‑1 (MQ=255) gTTGGTGAGGGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtgt < 1:10050/76‑1 (MQ=255) gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtgt < 1:2670796/76‑1 (MQ=255) gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtgt > 1:1369158/1‑76 (MQ=255) gtGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGtgtg < 2:1977885/75‑1 (MQ=255) gCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGCGAATATGTTTAGTGTGac > 2:2264203/1‑75 (MQ=255) ctaaGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAt < 1:3123911/73‑1 (MQ=255) cTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAt > 2:3650462/1‑76 (MQ=255) cTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAt > 1:3047312/1‑76 (MQ=255) cTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAt < 2:1450595/76‑1 (MQ=255) tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAtt > 2:4241054/1‑76 (MQ=255) tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAtt > 2:3378680/1‑76 (MQ=255) gAGCGGTTGGTGCTGCCTCGACCATCGGCATGAGGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAttt < 2:1180453/76‑1 (MQ=255) gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGATTAGTGTGAAttt > 1:1707507/1‑76 (MQ=255) aGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTg < 1:4122933/76‑1 (MQ=255) ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTgcgc > 2:1245435/1‑76 (MQ=255) ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAt < 2:1301462/76‑1 (MQ=255) ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAt > 2:4489379/1‑76 (MQ=255) tgggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAt < 1:3890672/74‑1 (MQ=255) tGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCACAtt > 2:3949809/1‑76 (MQ=255) gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTcc < 1:4489379/76‑1 (MQ=255) tgctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCt < 2:868985/76‑1 (MQ=255) tgctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCt > 1:1043654/1‑76 (MQ=255) tgctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCt > 1:470347/1‑76 (MQ=255) gctgcCTCGACCATCGGCACGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTg < 2:4644432/76‑1 (MQ=255) ctgcCTCGGCCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTgg < 2:3257349/76‑1 (MQ=255) ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTgg > 1:3923186/1‑76 (MQ=255) tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTTGc > 2:606711/1‑76 (MQ=255) tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc < 2:3525503/76‑1 (MQ=255) tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc > 1:2985141/1‑76 (MQ=255) gcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcc < 2:2527249/76‑1 (MQ=255) cgtCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAGTCCTGGccc > 1:1831491/3‑76 (MQ=255) tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa > 1:4365839/1‑76 (MQ=255) ccATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCa < 1:2066187/76‑1 (MQ=255) ccATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCa > 2:2914574/1‑76 (MQ=255) cATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg > 2:939604/1‑76 (MQ=255) cATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg < 1:2747418/76‑1 (MQ=255) cATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg < 2:2710499/76‑1 (MQ=255) cATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg > 1:4129508/1‑76 (MQ=255) cATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCACAGTccg > 1:4132259/1‑74 (MQ=255) aTCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg < 1:240410/76‑1 (MQ=255) aTCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg < 1:41912/76‑1 (MQ=255) cGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta < 1:799461/76‑1 (MQ=255) cGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta > 2:3847146/1‑76 (MQ=255) cGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta > 1:1976852/1‑76 (MQ=255) cGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta < 1:4773799/76‑1 (MQ=255) cGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCACAGTCAGGta > 1:1984096/1‑76 (MQ=255) cGGCAGGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta < 2:1496677/76‑1 (MQ=255) gCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaat > 1:3003752/1‑76 (MQ=255) gCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaat < 2:4144806/76‑1 (MQ=255) gCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaat < 2:1781804/76‑1 (MQ=255) cATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata < 1:3175746/76‑1 (MQ=255) aTGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa > 1:3504860/1‑76 (MQ=255) aTGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa < 2:4062499/76‑1 (MQ=255) tGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAc > 1:1204610/1‑76 (MQ=255) gACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt < 1:4456369/76‑1 (MQ=255) gACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt < 2:834126/76‑1 (MQ=255) gcGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTc < 2:781336/75‑1 (MQ=255) aCGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTc > 1:2479432/1‑76 (MQ=255) cGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCa < 2:531875/76‑1 (MQ=255) ggCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc < 1:3013761/76‑1 (MQ=255) ggCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc < 2:4603406/76‑1 (MQ=255) gCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAcc > 2:2535930/1‑76 (MQ=255) gCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAcc < 1:2177025/76‑1 (MQ=255) ccAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa < 1:3203775/69‑1 (MQ=255) ccAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa > 2:3203775/1‑69 (MQ=255) aGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt < 1:907621/76‑1 (MQ=255) caCCGAGGGAAAGCGCGAATATGCTTAGTGAGAATTTGCGGGTTCCTGGCCCCAGTCAGGTAATAACTCACCtttt > 2:3516480/2‑76 (MQ=255) aCCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg < 1:2361640/76‑1 (MQ=255) aCCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg > 2:1441806/1‑76 (MQ=255) ccGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTTGTCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt < 1:4491304/76‑1 (MQ=255) cGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTc < 1:2224534/76‑1 (MQ=255) gAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg < 1:4020785/76‑1 (MQ=255) gAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg < 2:3923186/76‑1 (MQ=255) gAGGGAAAGCGTGAATATGTTGAGTGGGAATTTGCGCAGTCCTGGCCCCAGGCAGGTGATAACTCACCTtgtggcg < 2:3539254/76‑4 (MQ=255) gggAAAGCGTGAATATGTCAAGTGTTAATTTGCGCATTCCTGGCCCCAGTCAGTTAATACCTCACCTTTTTTCgag < 2:2245424/76‑1 (MQ=255) ggAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgaga > 1:2758406/1‑76 (MQ=255) ggAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgaga < 2:2164053/76‑1 (MQ=255) gAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa > 1:2470675/1‑76 (MQ=255) gAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa < 2:1758485/76‑1 (MQ=255) gAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa > 2:4006120/1‑76 (MQ=255) gAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa < 1:4158603/76‑1 (MQ=255) aaaGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAg > 1:2876823/1‑76 (MQ=255) | ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG > NC_000913/3686700‑3686844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |