BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,896,324	+G	coding (698/711 nt)	b3718 ←	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,896,324	1	.	G	100.0%	253.0 / NA	97	V233A (GTG→GCG)	b3718	putative isomerase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (59/38); total (59/38)

Only 100 of 102 total aligned reads displayed.

ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTA  >  NC_000913/3896250‑3896392

atGAGAACAGGCCGGAGCGTCACTAACACATCCCGCCTTATTTCGTAAGCTGAAGCGAGTGCTAATTCTGCGGCA‑c                                                                     >  2:3459164/1‑76 (MQ=255)

atGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCag                                                                      >  2:4129926/1‑75 (MQ=255)

 tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                     >  1:1292563/1‑74 (MQ=255)

 tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGATAATTCTGCGGCagg                                                                     >  1:2015743/1‑74 (MQ=255)

 agagaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                     <  1:4089/75‑3 (MQ=255)

     aaCAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTACTTCTGCGGCAGCGGCt                                                                 >  1:1185035/1‑76 (MQ=255)

        aGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCtttt                                                              >  1:1666144/1‑75 (MQ=255)

        aGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCtttt                                                              >  1:4588825/1‑75 (MQ=255)

        aGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTa                                                              >  1:4203309/1‑76 (MQ=255)

         ggCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                                                             <  1:364588/76‑1 (MQ=255)

          gCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATc                                                            >  1:1291236/1‑76 (MQ=255)

           ccGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                                                           <  1:2115106/76‑1 (MQ=255)

           ccGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                                                           <  2:168596/76‑1 (MQ=255)

            cGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg                                                          >  1:717548/1‑76 (MQ=255)

            cGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg                                                          >  1:2603790/1‑76 (MQ=255)

             ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                         <  1:3688032/76‑1 (MQ=255)

             ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                         >  2:199992/1‑76 (MQ=255)

             ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                         >  2:1801231/1‑76 (MQ=255)

               aGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGa                                                       >  1:3511857/1‑76 (MQ=255)

                 cGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAta                                                     <  2:1593485/76‑2 (MQ=255)

                  gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTTTCAGCGATTa                                                    >  2:4830540/1‑76 (MQ=255)

                  gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                    >  1:2963346/1‑76 (MQ=255)

                  gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                    >  1:3891944/1‑76 (MQ=255)

                   tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAc                                                   >  2:3395148/1‑76 (MQ=255)

                   tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAc                                                   <  1:2958114/76‑1 (MQ=255)

                    aaTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                  >  1:650334/1‑76 (MQ=255)

                    aaTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                  <  1:1363993/76‑1 (MQ=255)

                    aaTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                  >  2:2969578/1‑76 (MQ=255)

                      ttCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCAt                                                <  2:4670263/76‑1 (MQ=255)

                      ttCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCAt                                                <  1:10995/76‑1 (MQ=255)

                        cacacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCCTcc                                              >  2:1322545/1‑75 (MQ=255)

                         acacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAg                                             <  2:3701958/76‑1 (MQ=255)

                          cacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGc                                            <  1:1748050/76‑1 (MQ=255)

                          cacaTCCGGCCTTATTGCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGc                                            <  2:2151753/76‑1 (MQ=255)

                           acaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCg                                           <  2:870112/76‑1 (MQ=255)

                            caTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGa                                          <  1:26123/76‑1 (MQ=255)

                             aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGaa                                         <  1:2838452/76‑1 (MQ=255)

                             aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGaa                                         <  1:615662/76‑1 (MQ=255)

                             aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGaa                                         >  1:1336736/1‑76 (MQ=255)

                             aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGaa                                         >  1:984970/1‑76 (MQ=255)

                              tCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                        <  2:652031/76‑1 (MQ=255)

                              tCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                        >  2:1516263/1‑76 (MQ=255)

                               ccGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCCGCGCAgg                                       >  2:3577345/1‑76 (MQ=255)

                                cGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGa                                      >  1:3194871/1‑76 (MQ=255)

                                 ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCCTCCGCGCAGGAt                                     >  2:4694744/1‑76 (MQ=255)

                                 ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAt                                     >  1:1796685/1‑76 (MQ=255)

                                  gCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATg                                    >  2:3703187/1‑76 (MQ=255)

                                   ccTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGAAGAGGCTTTATAAGCGATTACCATCAGCGAAGGATGc                                   >  2:4101822/1‑76 (MQ=255)

                                    cTTATTTCTTAAGCTTACCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCa                                  <  1:2311719/76‑1 (MQ=255)

                                    cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCa                                  >  1:3142221/1‑76 (MQ=255)

                                    cTTATTTCTTAAGATTAACCGAGAGCTAATTCTGCGGCAGAGGCTTTATCAGCGAATAACATCAGCCAAGGAGGCa                                  >  1:1068158/1‑76 (MQ=255)

                                     ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAg                                 >  2:2354043/1‑76 (MQ=255)

                                     ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATGACCATCAGCGAAGGCTCCAg                                 >  2:1501480/1‑76 (MQ=255)

                                      tATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc                                <  2:444700/76‑1 (MQ=255)

                                      tATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc                                >  2:1942678/1‑76 (MQ=255)

                                      tATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACACTCAACGAAGGACGCacc                                >  2:190482/1‑74 (MQ=255)

                                        tttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTg                              <  1:768999/76‑1 (MQ=255)

                                        tttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTg                              <  2:2895104/76‑1 (MQ=255)

                                          tCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCa                            >  2:1519282/1‑76 (MQ=255)

                                          tCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCa                            <  2:3300893/76‑1 (MQ=255)

                                            ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAac                          <  1:1757609/76‑1 (MQ=255)

                                            ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAac                          <  1:361858/76‑1 (MQ=255)

                                             tAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTCACAGCAGCGGAGGATGCAGCTGCaaaa                         >  1:1054053/1‑74 (MQ=255)

                                             tAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCcaca                         >  2:4186016/1‑76 (MQ=255)

                                             tAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca                         >  2:2877983/1‑76 (MQ=255)

                                              aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                        <  1:846948/76‑1 (MQ=255)

                                              aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                        <  1:199992/76‑1 (MQ=255)

                                              aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                        >  1:3756412/1‑76 (MQ=255)

                                               aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg                       >  2:3332248/1‑76 (MQ=255)

                                               aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg                       <  1:3048393/76‑1 (MQ=255)

                                                gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCCTCAGCGAAGGATGCAGCTGCAACACgg                      >  2:3670596/1‑76 (MQ=255)

                                                gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACgg                      <  2:2928696/76‑1 (MQ=255)

                                                gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACgg                      >  2:2718544/1‑76 (MQ=255)

                                                gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACgg                      >  2:3487540/1‑76 (MQ=255)

                                                gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCCGCAacaccg                      >  2:4260759/1‑74 (MQ=255)

                                                  ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                    >  2:3984917/1‑76 (MQ=255)

                                                  ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                    <  1:3084634/76‑1 (MQ=255)

                                                   tAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAg                   >  2:4365340/1‑76 (MQ=255)

                                                   tAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCCGCAACACGCAAg                   >  2:2162682/1‑76 (MQ=255)

                                                     aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCt                 >  2:2574552/1‑76 (MQ=255)

                                                      ccGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCCGCGAAGGATGCAGCTGCAACACGGAAGCTg                >  2:3634440/1‑76 (MQ=255)

                                                      ccGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGCAGGATGCAGCTGCAACACGGAAGCTg                >  1:3532975/1‑76 (MQ=255)

                                                      ccGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTg                >  2:3637798/1‑76 (MQ=255)

                                                       cGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTgg               >  1:1740072/1‑76 (MQ=255)

                                                       cGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTgg               <  2:2850216/76‑1 (MQ=255)

                                                       cGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCCgg               >  2:654280/1‑76 (MQ=255)

                                                        gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa              >  1:3205289/1‑76 (MQ=255)

                                                        gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa              >  1:3453159/1‑76 (MQ=255)

                                                        gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa              <  2:2906421/76‑1 (MQ=255)

                                                        gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa              >  1:1986782/1‑76 (MQ=255)

                                                         aGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGaa             >  1:204757/1‑76 (MQ=255)

                                                         aGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCCGGaa             >  2:868747/1‑76 (MQ=255)

                                                          gTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAAc            >  1:588535/1‑76 (MQ=255)

                                                            gCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACAt          <  2:1191821/76‑1 (MQ=255)

                                                             cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc         <  1:3648873/76‑1 (MQ=255)

                                                             cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc         <  1:3985953/76‑1 (MQ=255)

                                                              tAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCt        <  2:3033528/76‑1 (MQ=255)

                                                               aaTTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCtt       >  1:3122163/1‑76 (MQ=255)

                                                                   cTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGt   <  2:3695804/76‑1 (MQ=255)

                                                                    tGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGACGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTa  >  2:3274957/1‑76 (MQ=255)

ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTA  >  NC_000913/3896250‑3896392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`